Multivariate analysis of the salinity-induced alterations in morphology, physiology, nodulation, and yield in two contrasting mungbean varieties

Ganesh D. MANKAR; Uttam R. WAYASE; Deepak B. SHELKE; Kiran B. RASKAR; Pankaj S. MUNDADA; Mahendra L. AHIRE; Tukaram D. NIKAM; Rajkumar B. BARMUKH

doi:10.55779/nsb15211365

Authors

Ganesh D. MANKAR Post Graduate Research Centre, Modern College of Arts, Science and Commerce (Autonomous), Shivajinagar, Pune - 5, affiliated to Savitribai Phule Pune University, Pune - 7, MS (IN)
Uttam R. WAYASE Post Graduate Research Centre, Modern College of Arts, Science and Commerce (Autonomous), Shivajinagar, Pune - 5, affiliated to Savitribai Phule Pune University, Pune - 7, MS (IN)
Deepak B. SHELKE Department of Botany, Amruteshwar Arts, Commerce and Science College, Vinzar, Velhe, Pune - 4112213, MS (IN)
Kiran B. RASKAR Post Graduate Research Centre, Modern College of Arts, Science and Commerce (Autonomous), Shivajinagar, Pune - 5, affiliated to Savitribai Phule Pune University, Pune - 7, MS (IN)
Pankaj S. MUNDADA Yashavantrao Chavan Institute of Science, Department of Biotechnology, Satara 415001, MS (IN)
Mahendra L. AHIRE Yashavantrao Chavan Institute of Science, Department of Botany, Satara 415001, MS (IN)
Tukaram D. NIKAM Savitribai Phule Pune University, Department of Botany, Pune 411007, MS (IN)
Rajkumar B. BARMUKH Post Graduate Research Centre, Modern College of Arts, Science and Commerce (Autonomous), Shivajinagar, Pune - 5, affiliated to Savitribai Phule Pune University, Pune - 7, MS (IN)

DOI:

https://doi.org/10.55779/nsb15211365

Keywords:

mungbean, nodulation, physiological, salt stress, yield

Abstract

Changes were estimated in the morphology, physiology, photosynthesis, nodulation, and yield in two mungbean varieties ‘PKV AKM 12-28’ and ‘VBN (Gg)3’ under salt stress (0, 75, 100, and 125 mM NaCl) for 15, 30, and 45 days. Multivariate modelling was used to analyse results to explore complex data and to visualize time and concentration-dependent modulations. Principal component analysis showed modulations in morpho-physiological attributes such as shoot length, root length, the number of secondary branches, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight and leaf area; photosynthetic attributes such as chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll, total carotene and total anthocyanine content; nodulation attributes such as nodules per plant, size of the nodule, and fresh weight per nodule, and yield attributes such as number of pods per plant, fresh weight per pod, and seed characteristics such as the number of seeds per pod and fresh weight per 1000 seeds are key traits affected by salt stress and can be used as indicators. Discriminant analysis identiﬁed modulations in morpho-physiological attributes such as root length, leaf area, root fresh weight, shoot fresh weight, shoot dry weight, shoot length and photosynthetic attributes such as chlorophyll-a content, and mean nodule weight as discriminating variables at different salt concentrations. Besides, it identified modulations in morpho-physiological attributes such as root length, root fresh weight, photosynthetic attributes such as total anthocyanin content and total chlorophyll content, nodulation attribute such nodule size and nodule weight, and yield attributes such as pod number and number of seeds per pod are discriminating variables at various durations of salt stress. Principal component analysis and discriminant analysis identiﬁed ‘PKV-AKM 12-28’ as salt-tolerant and ‘VBN (Gg)3’ as salt-susceptible varieties. Multiple correlation analysis identified significant correlations among morphological, physiological, photosynthetic, nodulation and yield parameters.

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References

Abd-Alla MH, Vuong TD, Harper JE (1998). Genotypic differences in dinitrogen fixation response to NaCl stress in intact and grafted soybean. Crop Science 38:72-77. https://doi.org/10.2135/cropsci1998.0011183X003800010013x.

Ahmed S (2009). Effect of soil salinity on the yield and yield components of mungbean. Pakistan Journal of Botany 41:263-268.

Ali M, Gupta S (2012). Carrying capacity of Indian agriculture: pulse crops. Current Science 102:874-881.

Arif Y, Singh P, Siddiqui H, Bajguz A, Hayat S (2020). Salinity induced physiological and biochemical changes in plants: An omic approach towards salt stress tolerance. Plant Physiology and Biochemistry 156:64-77. https://doi.org/10.1016/j.plaphy.2020.08.042

Arnon DI (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24:1-15. https://doi.org/10.1104/pp.24.1.1

Chunthaburee S, Dongsansuk A, Sanitchon J, Pattanagul W, Theerakulpisut P (2015). Physiological and biochemical parameters for evaluation and clustering of rice cultivars differing in salt tolerance at seedling stage. Saudi Journal of Biological Sciences 23:467-477. https://doi.org/10.1016/j.sjbs.2015.05.013

Chutipaijit S, Cha-Um S, Sompornpailin K (2009). Differential accumulations of proline and flavonoids in indica rice varieties against salinity. Pakistan Journal of Botany 41:2497-2506.

Dhingra KK, Dhillon MS, Grewal DS, Sharma K (1991). Performance of maize and mungbean intercropping in different planting patterns and row orientations. Indian Journal of Agronomy 36:207-212.

Dolatabadian A, Modarres SS, Ghanati F (2011). Effect of salinity on growth, xylem structure and anatomical characteristics of soybean. Notulea Scientia Biologicae 3:41-45. https://doi.org/10.15835/nsb315627

Elahi NN, Mustafa S, Mirza JI (2004). Growth and nodulation of mungbean (Vigna radiata [L.] Wilczek) as affected by sodium chloride. Journal of Research 15:139-143.

Elsheikh EAE, Wood M (1995) Nodulation and N2 fixation by soybean inoculated with salt-tolerant rhizobia or salt-sensitive bradyrhizobia in saline soil. Soil Biology and Biochemistry 27:657-661. https://doi.org/10.1016/0038-0717(95)98645-5

FAO (2015). FAOSTAT. Retrieved 2021 March 30 from: Agric database http//apps.fao.org.

Fisarakis I, Chartzoulakis K, Stavrakas D (2001). Response of Sultana vines (V. vinifera L.) on six rootstocks to NaCl salinity exposure and recovery. Agricultural Water Management 51:13-27. https://doi.org/10.1016/S0378-3774(01)00115-9

Gadallah MAA (1999). Effects of proline and glycinebetaine on Vicia faba responses to salt stress. Biologia Plantarum 42:249-257. https://doi.org/10.1023/A:1002164719609

Ghosh S, Mitra S, Paul A (2015). Physiochemical studies of sodium chloride on mungbean (Vigna radiata L. Wilczek) and its possible recovery with spermine and gibberellic acid. The Scientific World Journal 858016. https://doi.org/10.1155/2015/858016.

Gill KS (1979). Effect of soil salinity on grain filling and grain development in barley. Biologia Plantarum 21:241-244. https://doi.org/10.1007/BF02902204

Hamani AKM, Wang G, Soothar MK, Shen X, Gao Y, Qiu R, Mehmood F (2020). Responses of leaf gas exchange attributes, photosynthetic pigments and antioxidant enzymes in NaCl-stressed cotton (Gossypium hirsutum L.) seedlings to exogenous glycine betaine and salicylic acid. BMC Plant Biology 20:1-14. https://doi.org/10.1186/s12870-020-02624-9.

Hammer Ø, Harper DAT, Ryan PD (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4:4-9. https://palaeo-electronica.org/2001_1/past/past.pdf

Hasanuzzaman M, Hossain MA, da Silva JAT, Fujita M (2012). Plant response and tolerance to abiotic oxidative stress: antioxidant defense is a key factor, In Crop stress and its management: perspectives and strategies, Springer, Dordrecht pp 261-315. https://doi.org/10.1007/978-94-007-2220-0_8

Hasegawa PM (2013). Sodium (Na+) homeostasis and salt tolerance of plants. Environmental and Experimental Botany 92:19-31. https://doi.org/10.1016/j.envexpbot.2013.03.001.

Hester MW, Mendelssohn IA, McKee KL (2001). Species and population variation to salinity stress in Panicum hemitomon, Spartina patens, and Spartina alterniflora: Morphological and physiological constraints. Environmental and Experimental Botany 46:277-297. https://doi.org/10.1016/S0098-8472(01)00100-9

Hoagland DR, Arnon DI (1950). The water-culture method for growing plants without soil. Circular. California agricultural Experiment Station, 347. (2nd edit).

Kamrul HM, Islam MS, Islam MR., Ismaan HN, Sabagh AEL (2018). Germination and early seedling growth of mungbean (Vigna radiata L.) as influenced by salinity. Azarian Journal of Agriculture 5:79-59.

Keating BA, Fisher MJ (1985). Comparative tolerance of tropical grain legumes to salinity. Australian Journal of Agricultural Research 36:373-383. https://doi.org/10.1071/AR9850373

Kijne JW, Stacey G, Burris RH, Evans HJ (1992). Biological nitrogen fixation. In: Stacey G, Burris RH, Evans HJ (Eds). Chapman Hall, London pp 349-398.

Koyro HW (2006). Effect of salinity on growth, photosynthesis, water relations and solute composition of the potential cash crop halophyte Plantago coronopus (L.). Environmental and Experimental Botany 56:136-146. https://doi.org/10.1016/j.envexpbot.2005.02.001

Kumar RA, Singh MP, Kumar S (2012). Effect of salinity on germination, growth, yield and yield attributes of wheat. International Journal of Scientific & Technology Research 1:19-23.

Maas EV, Poss JA, Hoffman GJ (1986). Salinity sensitivity of sorghum at three growth stages. Irrigation Science 7:1-11. https://doi.org/10.1007/BF00255690

Maclachlan S, Zalik S (1963). Plastid Structure, chlorophyll concentration, and free amino acid composition of a chlorophyll mutant of barley. Canadian Journal of Botany 41:1053-1062. https://doi.org/10.1139/b63-088

Mahmood A, Athar M, Qadri R, Mahmood N (2008). Effect of NaCl salinity on growth, nodulation and total nitrogen content in Sesbania sesban. Agriculturae Conspectus Scientificus 73:137-141.

Mancinelli AL (1984). Photoregulation of anthocyanin synthesis. Plant Physiology 75:447-453. https://doi.org/10.1104/pp.75.2.447

Michalski R, Kaufman K (1997). Data mining and knowledge discovery: A review of issues and a multi-strategy approach. Mach Learn Data Min Methods 1-42.

Mirza JI, Tariq R (1993). Effect of salinity on growth, seed yield and nodulation of Cicer Arietinum. Pakistan Journal of Botany 25:47-50.

Mirza JI, Tariq R (1993). The growth and nodulation of Trifolium alexandrinum as affected by salinity. Biologia Plantarum 35:289-292.

Morales SG, Trejo-Téllez LI, Merino FCG, Caldana C, Espinosa-Victoria D, Cabrera BEH (2012). Growth, photosynthetic activity, and potassium and sodium concentration in rice plants under salt stress. Acta Scientiarum Agronomy 34:317-324.

Muchate NS, Nikalje GC, Rajurkar NS, Suprasanna P, Nikam TD (2016). Physiological responses of the halophyte Sesuvium portulacastrum to salt stress and their relevance for saline soil bio-reclamation. Flora 224:96-105. https://doi.org/10.1016/j.flora.2016.07.009

Mujunen SP, Minkkinen P, Holmbom B, Oikari A (1996). PCA and PLS methods applied to ecotoxicological data: ecobalance project. Journal of Chemometrics 10:411-424. https://doi.org/10.1002/(SICI)1099-128X(199609)10:5/6<411::AID-CEM441>3.0.CO;2-7

Munns R (2005). Genes and salt tolerance: Bringing them together. New Phytologist 167:645-663. https://doi.org/10.1111/j.1469-8137.2005.01487.x.

Najar R, Aydi S, Sassi-Aydi S, Zarai A, Abdelly C (2019). Effect of salt stress on photosynthesis and chlorophyll fluorescence in Medicago truncatula. Plant Biosystems 153:88-97. https://doi.org/10.1080/11263504.2018.1461701.

Panta S, Flowers T, Lane P, Doyle R, Haros G, Shabala S (2014). Halophyte agriculture: Success stories. Environmental and Experimental Botany 107:71-83. https://doi.org/10.1016/j.envexpbot.2014.05.006

Rahman MM, Habib MA, Sikdar MSI, Md S, Islam MS (2016). Evaluation of mungbean genotypes for salt tolerance at seedling stage and alleviation of saline stress by gypsum. Fundamental and Applied Agriculture 1:39-43.

Rahneshan Z, Nasibi F, Moghadam AA (2018). Effects of salinity stress on some growth, physiological, biochemical parameters and nutrients in two pistachio rootstocks. Journal of Plant Interaction 13:73-82. https://doi.org/10.1080/17429145.2018.1424355

Ram HH, Singh HG (1993). Rapeseed-Mustard. Crop Breeding and Genetics. Kalyani Publ New Delhi.

Randhir R, Lin YT, Shetty K (2004). Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors. Process Biochemistry 39:637-646. https://doi.org/10.1016/S0032-9592(03)00197-3

Rao DLN, Giller KE, Yeo AR, Flowers TJ (2002). The effects of salinity and sodicity upon nodulation and nitrogen fixation in chickpea (Cicer arietinum). Annals of Botany 89:563-570. https://doi.org/10.1093/aob/mcf097

Raza MA, Saeed A, Munir H, Ziaf K, Shakeel A, Saeed N, ... Rehman F (2017). Screening of tomato genotypes for salinity tolerance based on early growth attributes and leaf inorganic osmolytes. Archives of Agronomy and Soil Science 63:501-512. https://doi.org/10.1080/03650340.2016.1224856.

Sarabi B, Bolandnazar S, Ghaderi N, Tabatabaei SJ (2016) Multivariate analysis as a tool for studying the effects of salinity in different melon landraces at germination stage. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44:264-271. https://doi.org/10.15835/nbha44110234.

Sarin MN, YCJ, KSG (1975). Salt tolerance of wheat and barley varieties. Proc Symposium. New Developments in the field of salt affected soils. IS.sS Carro 647-652.

Satti SME, Lopez M (1994). Effect of increasing potassium levels for alleviating sodium chloride stress on the growth and yield of tomato. Communications in Soil Science and Plant Analysis 25:2807-2823. https://doi.org/10.1080/00103629409369227

Sehrawat N, Yadav M, Bhat K, Sairam R, Jaiwal P (2015). Effect of salinity stress on mungbean [Vigna radiata (L.) Wilczek] during consecutive summer and spring seasons. Journal of Agricultural Sciences, Belgrade 60:23-32. https://doi.org/10.2298/JAS1501023S

Shelke DB, Pandey M, Nikalje GC, Zaware BN, Suprasanna P, Nikam TD (2017). Salt responsive physiological, photosynthetic and biochemical attributes at early seedling stage for screening soybean genotypes. Plant Physiology and Biochemistry 118:519-528. https://doi.org/10.1016/j.plaphy.2017.07.013

Sherif FK, Raslan MM, El-Sammak FZ (2007). Effect of Gamma radiation on some morphological and biochemical characters of Tagetes erecta grown in saline soil. Alexandria Science Exchange Journal 28:54-67. 10.21608/ASEJAIQJSAE.2007.1851

Shrivastava P, Kumar R (2015). Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences 22:123-131. https://doi.org/10.1016/j.sjbs.2014.12.001.

Simeonov V, Stratis JA, Samara C, Zachariadis G, Voutsa D, Anthemidis A, ... Kouimtzis T (2003). Assessment of the surface water quality in Northern Greece. Water Research 37:4119-4124. https://doi.org/10.1016/S0043-1354(03)00398-1

Singh AK, Singh RA, Kumar S (2008). Influence of salinity on seedling growth and metabolism in maize genotypes. Indian Journal of Plant Physiology 13:95-99.

Singh KP, Malik A, Mohan D, Sinha S (2004). Multivariate statistical techniques for the evaluation of spatial and temporal variations in water quality of Gomti River (India) - A case study. Water Research 38:3980-3992. https://doi.org/10.1016/j.watres.2004.06.011

Singla R, Garg N (2005). Influence of salinity on growth and yield attributes in chickpea cultivars. Turkish Journal of Agriculture and Forestry 29:231-235.

Singleton PW, Bohlool BB (1984). Effect of salinity on nodule formation by soybean. Plant Physiology 74:72-76. https://doi.org/10.1104/pp.74.1.72

Sinha S, Basant A, Malik A, Singh KP (2009a). Iron-induced oxidative stress in a macrophyte: A chemometric approach. Ecotoxicology and Environmental Safety 72:585-595. https://doi.org/10.1016/j.ecoenv.2008.04.017

Sinha S, Basant A, Malik A, Singh KP (2009b). Multivariate modelling of chromium-induced oxidative stress and biochemical changes in plants of Pistia stratiotes L. Ecotoxicology 18:555-566. https://doi.org/10.1007/s10646-009-0313-6

Steduto P, Albrizio R, Giorio P, Sorrentino G (2000). Gas-exchange response and stomatal and non-stomatal limitations to carbon assimilation of sunflower under salinity. Environmental and Experimental Botany 44:243-255. https://doi.org/10.1016/S0098-8472(00)00071-X

Sultana N, Ikeda T, Itoh R (1999). Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environmental and Experimental Botany 42:211-220. https://doi.org/10.1016/S0098-8472(99)00035-0

Taffouo VD, Wamba OF, Youmbi E, Nono GV, Akoa A (2010). Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranea (L.) Verdc.) landraces grown under saline conditions. International Journal of Botany 6:53-58.

Taïbi K, Taïbi F, Ait Abderrahim L, Ennajah A, Belkhodja M, Mulet JM (2016). Effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidant defence systems in Phaseolus vulgaris L. South African Journal of Botany 105:306-312. https://doi.org/10.1016/j.sajb.2016.03.011.

Taiz L, Zeiger E (1998). Plant physiology. 2nd edn. Sinauer Associates Publishers, Sunderland, Massachusetts.

Tang D, Dong Y, Ren H, Li L, He C (2014). A review of phytochemistry, metabolite changes, and medicinal uses of the common food mung bean and its sprouts (Vigna radiata). Chemistry Central Journal 8:1-9. https://doi.org/10.1186/1752-153X-8-4

Turan MA, Turkmen N, Taban N (2007). Effect of NaCl on stomatal resistance and proline, chlorophyll, Na, Cl and K concentrations of lentil plants. Journal of Agronomy 6:378-381.

Walsh KB (1995). Physiology of the legume nodule and its response to stress. Soil Biology and Biochemistry 27:637-655. https://doi.org/10.1016/0038-0717(95)98644-4

Xu FJ, Jin CW, Liu WJ, Zhang YS, Lin XY (2011). Pretreatment with H2O2 alleviates aluminum-induced oxidative stress in wheat seedlings. Journal of Integrative Plant Biology 53:44-53. https://doi.org/10.1111/j.1744-7909.2010.01008.x.