Silicon alleviates PEG-induced osmotic stress in finger millet by regulating membrane damage, osmolytes, and antioxidant defense

Authors

  • Pankaj S. MUNDADA Savitribai Phule Pune University, Department of Botany, Pune 411 007; Yashavantrao Chavan Institute of Science, Department of Biotechnology, Satara 415 001
  • Mitali M. SONAWANE Yashavantrao Chavan Institute of Science, Department of Biotechnology, Satara 415 001
  • Sumaiya S. SHAIKH Yashavantrao Chavan Institute of Science, Department of Botany, Satara 415 001
  • Vitthal T. BARVKAR Savitribai Phule Pune University, Department of Botany, Pune 411 007
  • S. ANIL KUMAR Vignan’s Foundation for Science, Technology and Research, Department of Biotechnology, Vadlamudi, Guntur 522 213
  • Suraj D. UMDALE Jaysingpur College, Department of Botany, Jaysingpur 416 101
  • Penna SUPRASANNA Bhabha Atomic Research Centre, Functional Plant Biology Section, Mumbai, 400 085
  • Rajkumar B. BARMUKH Modern College of Arts, Science and Commerce, Post Graduate Research Centre, Department of Botany, Shivajinagar, Pune
  • Tukaram D. NIKAM Savitribai Phule Pune University, Department of Botany, Pune 411 007
  • Mahendra L. AHIRE Yashavantrao Chavan Institute of Science, Department of Botany, Satara 415 001

DOI:

https://doi.org/10.55779/nsb14411097

Keywords:

antioxidative enzymes, finger millet, osmolytes, osmotic stress, PEG, silicon

Abstract

Drought restricts plant growth and productivity. Silicon has beneficial effects on imparting drought tolerance in plants. Present work was intended to evaluate the effect of Si on polyethylene glycol-6000 (PEG) induced osmotic stress in local landraces of finger millet. The seeds of stress-tolerant and stress-sensitive landraces of finger millet were treated with distilled water, 15% PEG, and PEG+Si (5-25 ppm). The ameliorative effect of Si was evaluated in terms of percentage seed germination, seedling growth, accumulation of osmolyte and activity of antioxidative enzymes. PEG-induced osmotic stress reduced seed germination, seedling growth, and augmented osmolyte accumulation. It also elevated the levels of antioxidant enzymes. The exogenous supplementation of silicon significantly improved seed germination as well as early seeding growth. Positive effects of Si were reflected in decline in malondialdehyde (MDA) content and improved glycine betaine content and antioxidant enzymes in PEG-induced stress tolerant as well as susceptible landraces. The Si-induced ameliorated effects on all the parameters studied were more pronounced in the stress-tolerant landrace (FM/ST/01) than the stress-sensitive landrace (FM/RT/01). These results clearly indicate advantageous effects of Si in relieving PEG-induced stress during seed germination and early seeding growth and suggest a possibility of better stand establishment by application of silicon containing fertilizer during seed sowing.

References

Afef O, Sourour A, Zoubeir C, Mounir R, Hajer SA, Mongi BY (2016). Silicon alleviates adverse effect of drought stress induced by polyethylene glycol (PEG 8000) on seed germination and seedling growth of durum wheat varieties. International Journal of Current Research 8:40847-40851.

Agarie S, Uchida H, Agata W, Kubota F, Kaufman PB (1998). Effects of silicon on transpiration and leaf conductance in rice plants (Oryza saliva L.). Plant Production Science 1:89-95. https://doi.org/10.1626/pps.1.89

Ahire ML, Lokhande VH, Kavi Kishor PB, Nikam TD (2012). Brinjal (Solanum melongena Linn.) varieties accumulate both Na+ and K+ under low NaCl stress, but excludes Na+ and accumulate K+ under high salt levels. The Asian and Australasian Journal of Plant Science and Biotechnology 6:1-6.

Ahire ML, Nikam TD (2011). Differential response of Brinjal (Solanum melongena Linn.) varieties to salinity stress in relation to seed germination and osmolytes accumulation. Seed Science and Biotechnology 5:29-35.

Ahire ML, Walunj PR, Kavi Kishor PB, Nikam TD (2013). Effect of sodium chloride induced stress on growth, proline, glycine betaine accumulation, antioxidative defense and bacoside A content in in vitro regenerated shoots of Bacopa monnieri (L.) Pennell. Acta Physiologiae Plantarum 35:1943-1953. https://doi.org/10.1007/s11738-013-1233-x

Almansouri M, Kinet JM, Lutts S (2001). Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant and Soil 23:243-254. https://doi.org/10.1023/A:1010378409663

Antony Ceasar S, Maharajan T, Ajeesh Krishna TP, Ramakrishnan M, Victor Roch G, Satish L, Ignacimuthu S (2018). Finger millet [Eleusine coracana (L.) Gaertn.] Improvement: current status and future interventions of whole genome sequence. Frontiers in Plant Science 9:1054. https://doi.org/10.3389/fpls.2018.01054

Bates LS, Waldren RP, Tearem ID (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39:205-207. https://doi.org/10.1007/BF00018060

Beyer WF, Fridovich I (1987). Assaying for superoxide dismutase activity: some large consequences of minor changes in condition. Analytical Biochemistry 161:559-566. https://doi.org/10.1016/0003-2697(87)90489-1

Biju S, Fuentes S, Gupta D (2017). Silicon improves seed germination and alleviates drought stress in lentil crops by regulating osmolytes, hydrolytic enzymes and antioxidant defense system. Plant Physiology and Biochemistry 119:250-264. https://doi.org/10.1016/j.plaphy.2017.09.001

Cakmak I, Marschner H (1992). Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiology 98:1222-1227. https://doi.org/10.1104/pp.98.4.1222

Datnoff LE, Snyder GH, Korndörfer GH (2001). Silicon in agriculture. Elsevier, New York.

de Oliveira RLL, de Mello Prado R, Felisberto G, Checchio MV, Gratão PL (2019). Silicon mitigates manganese deficiency stress by regulating the physiology and activity of antioxidant enzymes in sorghum plants. Journal of Soil Science and Plant Nutrition 19:524-534. https://doi.org/10.1007/s42729-019-00051-w

Devi PB, Vijayabharathi R, Sathyabama S, Malleshi NG, Priyadarisini VB (2014). Health benefits of finger millet (Eleusine coracana L.) polyphenols and dietary fiber: a review. Journal of Food Science and Technology 51:1021-1040. https://doi.org/10.1007/s13197-011-0584-9

Epstein E (1994) The anomaly of silicon in plant biology. Proceedings of the National Academy of Sciences of the United States of America 91:11-17. https://doi.org/10.1073/pnas.91.1.11

Epstein E (1999). Silicon. Annual Review of Plant Physiology and Plant Molecular Biology 50:641-664. https://doi.org/10.1146/annurev.arplant.50.1.641

Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomor M, Senhaji NS (2007). Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures. Acta Physiologiae Plantarum 29:95-102. https://doi.org/10.1007/s11738-006-0006-1

Gao X, Zou C, Wang L, Zhang F (2006). Silicon decreases transpiration rate and conductance from stomata of maize plants. Journal of Plant Nutrition 29:1637-1647. https://doi.org/10.1080/01904160600851494

Gong HJ, Chen KM, Chen GC, Wang SM, Zhang CL (2003). Effects of silicon on growth of wheat under drought. Journal of Plant Nutrition 26:1055-1063. https://doi.org/10.1081/PLN-120020075

Gossett DR, Millholon P, Lucas C (1994). Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Science 34:706-714. https://doi.org/10.2135/cropsci1994.0011183X003400030020x

Govind G, Harshavardhan VT, Patrica JK, Dhanalakshmi R, Senthil-Kumar M, Nese S, Udayakumar M (2009). Identification and functional validation of a unique set of droughts induced genes preferentially expressed in response to gradual water stress in peanut. Molecular Genetics and Genomics 281:591-605. https://doi.org/10.1007/s00438-009-0432-z

Grieve CM, Grattan SR (1983). Rapid assay for determination of water-soluble quaternary ammonium compounds. Plant and Soil 70:303-307. https://doi.org/10.1007/BF02374789

Gunes A, Pilbeam DJ, Inal A, Bagci EG, Coban S (2007). Influence of silicon on antioxidant mechanisms and lipid peroxidation in chickpea (Cicer arietinum L.) cultivars under drought stress. Journal of Plant Interaction 2:105-113. https://doi.org/10.1080/17429140701529399

Hameed A, Sheikh MA, Jamil A, Basra SMA (2013). Seed priming with sodium silicate enhances seed germination and seedling growth in wheat (Triticum aestivum L.) under water deficit stress induced by polyethylene glycol. Pakistan Journal of Life and Social Sciences 11:19-24.

Hattori T, Inanaga S, Araki H, An P, Morita S, Luxová M, Lux A (2005). Application of silicon enhanced drought tolerance in Sorghum bicolor. Physiology Plantarum 123:459-466. https://doi.org/10.1111/j.1399-3054.2005.00481.x

Heath RL, Packer L (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125:189-198. https://doi.org/10.1016/0003-9861(68)90654-1

Hemeda HM, Klein BP (1990). Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. Journal of Food Science 55:184-185. https://doi.org/10.1111/j.1365-2621.1990.tb06048.x

Imlay JA (2003). Pathways of oxidative damage. Annual Review of Microbiology 57:395-418. https://doi.org/10.1146/annurev.micro.57.030502.090938

Lekklar C, Chadchawan S, Boon-Long P, Pfeiffer W, Chaidee A (2019). Salt stress in rice: multivariate analysis separates four components of beneficial silicon action. Protoplasma 256:331-347. https://doi.org/10.1007/s00709-018-1293-2

Ma JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M (2006). A silicon transporter in rice. Nature 440:688-691. https://doi.org/10.1038/nature04590

Matoh T, Murata S, Takahashi E (1991). Effect of silicate application on photosynthesis of rice plants (in Japanese). Japanese Journal of Soil Science and Plant Nutrition 62:248-251.

Mundada PS, Jadhav SV, Salunkhe SS, Gurme ST, Umdale SD, Barmukh RB, Nikam TD, Ahire ML (2021). Silicon and plant responses under adverse environmental conditions. In: Husen A (Ed). Plant Performance Under Environmental Stress. Springer Nature, Switzerland AG pp 357-385.

Mundada PS, Nikam TD, Anil Kumar S, Umdale SD, Ahire ML (2020) Morpho-physiological and biochemical responses of finger millet (Eleusine coracana (L.) Gaertn.) genotypes to PEG-induced osmotic stress. Biocatalysis and Agricultural Biotechnology 23:101488. https://doi.org/10.1016/j.bcab.2019.101488

Mundada PS, Umdale SD, Nikam TD, Ahire ML (2019). Genetic diversity using RAPD markers, mineral composition and their correlation in selected local landraces of finger millet (Eleusine coracana (L.) Gaertn.). Vegetos 32:1-10. https://doi.org/10.1007/s42535-019-00001-y

Nagaraja A, Jayarame G, Krishnappa M, Gowda KTK (2008). GPU 28: a finger millet variety with durable blast resistance. Journal of Mycopathological Research 46:109-111.

Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell and Physiology 22:867-880. https://doi.org/10.1093/oxfordjournals.pcp.a076232

Nandini B, Ravishankar CR, Mahesha B, Shailaja H, Murthy KNK (2010). Study of correlation and path analysis in F2 population of finger millet. International Journal of Plant Sciences (Muzaffarnagar) 5:602-605.

Rezende RALS, Rodrigues FA, Soares JDR, Silveira HRO, Pasqual M, Dias GMG (2018). Salt stress and exogenous silicon influence physiological and anatomical features of in vitro-grown cape gooseberry. Ciência Rural 48:e20170176. https://doi.org/10.1590/0103-8478cr20170176

Rizwan M, Ali S, Ibrahim M, Farid M, Adrees M, Bharwana SA, Zia-ur-Rehman M, Qayyum MF, Abbas F (2015). Mechanisms of silicon-mediated alleviation of drought and salt stress in plants: a review. Environmental Science and Pollution Research 22:15416-15431. https://doi.org/10.1007/s11356-015-5305-x

Sakamma S, Umesh KB, Girish MR, Ravi SC, Satishkumar M, Bellundagi V (2018). Finger millet (Eleusine coracana L. Gaertn.) production system: status, potential, constraints and implications for improving small farmer’s welfare. Journal of Agricultural Sciences 10:162-179. https://doi.org/10.5539/jas.v10n1p162

Saud S, Li X, Chen Y, Zhang L, Fahad S, Hussain S, Sadiq A, Chen Y (2014). Silicon application increases drought tolerance of kentucky bluegrass by improving plant water relations and morphophysiological functions. Scientific World Journal 368694:1-10. https://doi.org/10.1155/2014/368694

Shen X, Zhou Y, Duan L, Li Z, Eneji AE, Li J (2010). Silicon effects on photosynthesis and antioxidant parameter of soybean seedlings under drought and ultraviolet-B radiation. Journal of Plant Physiology 167:1248-1252. https://doi.org/10.1016/j.jplph.2010.04.011

Shet RM, Gireesh C, Jagadeesha N, Lokesh GY, Jayarame G (2009). Genetic variability in segregating generation of interspecific hybrids of finger millet (Eleusine coracana (L.) Gaertn.). Environment and Ecology 27:1013-1016.

Shi Y, Zhang Y, Han W, Feng R, Hu Y, Guo J, Gong H (2016). Silicon enhances water stress tolerance by improving root hydraulic conductance in Solanum lycopersicum L. Frontiers in Plant Science 7:196. https://doi.org/10.3389/fpls.2016.00196

Shi Y, Zhang Y, Yao H, Wu J, Sun H, Gong H (2014). Silicon improves seed germination and alleviates oxidative stress of bud seedlings in tomato under water deficit stress. Plant Physiology and Biochemistry 78:27-36. https://doi.org/10.1016/j.plaphy.2014.02.009

Slama I, Ghnaya T, Savourѐ A, Abdelly C (2008) Combined effects of long-term salinity and soil drying on growth, water relations, nutrient status and proline accumulation of Sesuvium portulacastrum. Comptes Rendus Biologies 331:442-451. https://doi.org/10.1016/j.crvi.2008.03.006

Takahashi E, Ma JF, Miyake Y (1990). The possibility of silicon as an essential element for higher plants. Comments on Agricultural Food Chemistry 2:99-122.

Uma S, Prasad TG, Udayakumar M (1995). Genetic variability in recovery growth and synthesis of stress proteins in response to polyethylene glycol and salt stress in finger millet. Annals of Botany 76:43-49. https://doi.org/10.1006/anbo.1995.1076

Watanabe S, Kojima K, Ide Y, Sasaki S (2000). Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell Tissue and Organ Culture 63:199-206. https://doi.org/10.1023/A:1010619503680

Yin L, Wang S, Liu P, Wang W, Cao D, Deng X, Zhang S (2014) Silicon mediated changes in polyamine and 1-aminocyclopropane-1-carboxylic acid are involved in silicon-induced drought resistance in Sorghum bicolor L. Plant Physiology and Biochemistry 80:268-277. https://doi.org/10.1016/j.plaphy.2014.04.014

Published

2022-11-28

How to Cite

MUNDADA, P. S., SONAWANE, M. M., SHAIKH, S. S., BARVKAR, V. T., ANIL KUMAR, S., UMDALE, S. D., SUPRASANNA, P., BARMUKH, R. B. ., NIKAM, T. D., & AHIRE, M. L. (2022). Silicon alleviates PEG-induced osmotic stress in finger millet by regulating membrane damage, osmolytes, and antioxidant defense. Notulae Scientia Biologicae, 14(4), 11097. https://doi.org/10.55779/nsb14411097

Issue

Section

Research articles