Anticancer potentials of leaf, stem, and root extracts of Achyranthes aspera L.
DOI:
https://doi.org/10.15835/nsb12310764Keywords:
Achyranthes aspera L.; anticancer activity; cytotoxicity; LC-MS; MTT assayAbstract
Achyranthes aspera L. (Amaranthaceae), an herbaceous roadside weed in various parts of India, has several therapeutic uses, including the treatment against cancer. This investigation was undertaken to identify the bioactive compounds conferring cytotoxicity to the extracts from its biomass. The powdered leaf, stem, and root biomass was extracted separately in ethyl acetate, acetone, ethanol, and methanol. Each extract was tested against the HeLa cell line for the cytotoxicity, but the root-acetone extract was the most cytotoxic. This extract revealed eleven bands. The solution obtained from the ninth band (Rf = 0.87±0.06) exhibited more than 90% inhibition of HeLa cells. The LCMS analysis of this solution showed the presence of 37 compounds, out of which few compounds had been reported from different plants to possess cytotoxicity in various systems.
Metrics
References
Alkari S, Chaturvedi A (2015). Achyranthes aspera Linn: A reservoir of antioxidants. International Journal of Pharma and Bio Sciences 6(2):55-66. https://ijpbs.net/abstract.php?article=NDA4MQ==
Androutsopoulos V, Arroo RRJ, Hall JF, Surichan S, Potter GA (2008). Anti-proliferative and cytostatic effects of the natural product eupatorin on MDA-MB-468 human breast cancer cells due to CYP1-mediated metabolism. Breast Cancer Research 10(3):R39. https://doi.org/10.1186/bcr2090
Arora S, Tandon S (2014). Achyranthes aspera root extracts induce human colon cancer cell (COLO-205) death by triggering the mitochondrial apoptosis pathway and S phase cell cycle arrest. The Scientific World Journal 2014:129697. https://doi.org/10.1155/2014/129697
Baskar AA, Al. Numair KS, Alsaif MA, Ignacimuthu S (2012). In vitro antioxidant and anti-proliferative potential of medicinal plants used in traditional Indian medicine to treat cancer. Redox Report 17(4):145-156. https://doi.org/10.1179/1351000212Y.0000000017
Greenwell M, Rahman PKS (2015). Medicinal plants: Their use in anticancer treatment. International Journal of Pharmaceutical Science and Research 6(10):4103-4112. https://doi.org/10.13040/IJPSR.0975-8232.6(10).4103-12
Iwalewa EO, McGaw LJ, Naidoo V, Eloff JN (2007). Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. African Journal of Biotechnology 6(25):2868-2885. https://doi.org/10.5897/AJB2007.000-2457
Nagao T, Abe F, Kinjo J, Okabe H (2002). Antiproliferative constituents in plants 10. Flavones from the leaves of Lantana montevidensis Briq. and consideration of structure-activity relationship. Biological and Pharmaceutical Bulletin 25(7):875-879. https://doi.org/10.1248/bpb.25.875
Narayan C, Kumar A (2014). Antineoplastic and immunomodulatory effect of polyphenolic components of Achyranthes aspera (PCA) extract on urethane induced lung cancer in vivo. Molecular Biology Reports 41:179-191. https://doi.org/10.1007/s11033-013-2850-6
Singh BR, Yadav S, Singh S, Brind L (2011). Pharmacognostic investigations of Achyranthes aspera Linn. International Journal of Ayurvedic Medicine 2(4):145-152. https://www.ijam.co.in/index.php/ijam/article/view/76
Solowey E, Lichtenstein M, Sallon S, Paavilainen H, Solowey E, Lorberboum-Galski H (2014). Evaluating medicinal plants for anticancer activity. The Scientific World Journal 2014:721402. https://doi.org/10.1155/2014/721402
Subbarayan PR, Sarkar M, Impellizzeri S, Raymo F, Lokeshwar BL, Kumar P, … Ardalan B (2010). Anti-proliferative and anti-cancer properties of Achyranthes aspera: Specific inhibitory activity against pancreatic cancer cells. Journal of Ethnopharmacology 131(1):78-82. https://doi.org/10.1016/j.jep.2010.06.002
Subbarayan PR, Sarkar M, Nagaraja RS, Philip S, Kumar P, Altman N, … Lokeshwar BL (2012). Achyranthes aspera (Apamarg) leaf extract inhibits human pancreatic tumor growth in athymic mice by apoptosis. Journal of Ethnopharmacology 142(2):523-530. https://doi.org/10.1016/j.jep.2012.05.030
Tezuka Y, Stampoulis P, Banskota AH, Awale S, Tran KQ, Saiki I, Kadota S (2000). Constituents of the Vietnamese medicinal plant Orthosiphon stamineus. Chemical and Pharmaceutical Bulletin 48(11):1711-1719. https://doi.org/10.1248/cpb.48.1711
Valsaraj R, Pushpangadan P, Smitt UW, Adsersen A, Nyman U (1997). Antimicrobial screening of selected medicinal plants from India. Journal of Ethnopharmacology 58(2):75-83. https://doi.org/10.1016/s0378-8741(97)00085-8
Downloads
Published
How to Cite
Issue
Section
License
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
License:
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.
.png)
