Effect of Basal Media and Sugar Types on in Vitro Regeneration of Grammatophyllum speciosum Blume

  • Montakarn PIMSEN Prince of Songkla University, Department of Biology, Faculty of Science, Hat-Yai, Songkhla, 90112 (TH)
  • Kamnoon KANCHANAPOOM Prince of Songkla University, Department of Biology, Faculty of Science, Hat-Yai, Songkhla, 90112 (TH)

Abstract

Protocorms of Grammatophyllum speciosum Blume. were initiated from immature seeds on solid MS medium containing 15% (v/v) coconut water (CW) and 3% (w/v) sucrose. Protocorms, 2-4 mm in length were used as explants and subcultured to MS and VW (Vacin and Went, 1949) media containing 15% (v/v) CW and 2 or 3% (w/v) sucrose. Protocorms gave the highest formation of PLBs (protocorm-like bodies) at 3.1 PLBs/explant on MS solid medium containing 15% CW and 2% sucrose. For the test with different carbon sources, protocorms were cultured in liquid MS medium supplemented with 4 kinds of sugar, namely sucrose, glucose, sorbitol and mannitol at 2, 4, 6 or 8% (w/v) and cultured for 1, 2, 3 or 4 weeks. They were then transferred to MS solid medium containing 15% CW and 2% sucrose. Results revealed that after 4 weeks in MS liquid medium, sucrose and glucose had an inhibitory effect and 8% glucose gave a high percentage of protocorm browning. In contrast, sorbitol and mannitol were effective for protocorm regeneration and both sugar alcohols had a positive effect on the formation of PLBs and the development of PLBs into plantlets.

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Author Biographies

Montakarn PIMSEN, Prince of Songkla University, Department of Biology, Faculty of Science, Hat-Yai, Songkhla, 90112
Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Kamnoon KANCHANAPOOM, Prince of Songkla University, Department of Biology, Faculty of Science, Hat-Yai, Songkhla, 90112
Assoc. Prof. Dr. Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Published
2011-08-25
How to Cite
PIMSEN, M., & KANCHANAPOOM, K. (2011). Effect of Basal Media and Sugar Types on in Vitro Regeneration of Grammatophyllum speciosum Blume. Notulae Scientia Biologicae, 3(3), 101-104. https://doi.org/10.15835/nsb336153
Section
Research articles
CITATION
DOI: 10.15835/nsb336153