The Influence of Explant Types and Orientation on in Vitro Culture

  • Kamnoon KANCHANAPOOM Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla 90112 (TH)
  • Nararatn PROMSORN Prince of Songkla University, Faculty of Agro-Industry, Department of Industrial Biotechnology, Hat Yai, Songkhla 90112 (TH)

Abstract

Inflorescence, apical and lateral buds of Musa balbisiana ‘Kluai Hin’ (BBB group) were used to culture on MS medium supplemented with 22 μM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 μM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.

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Author Biographies

Kamnoon KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla 90112
Assoc. Prof. Dr. Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Nararatn PROMSORN, Prince of Songkla University, Faculty of Agro-Industry, Department of Industrial Biotechnology, Hat Yai, Songkhla 90112
Prince of Songkla University, Faculty of Agro-Industry, Department of Industrial Biotechnology, Hat Yai, Songkhla 90112, Thailand.
Published
2011-08-25
How to Cite
KANCHANAPOOM, K., & PROMSORN, N. (2011). The Influence of Explant Types and Orientation on in Vitro Culture. Notulae Scientia Biologicae, 3(3), 89-92. https://doi.org/10.15835/nsb336120
Section
Research articles
CITATION
DOI: 10.15835/nsb336120