Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) ‘Kluai Sa’ and ‘Kluai Leb Mu Nang’

  • Kamnoon KANCHANAPOOM Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla, 90112 (TH)
  • Korn KORAPATCHAIKUL Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla, 90112 (TH)

Abstract

Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group) ‘Kluai Sa’ and ‘Kluai Leb Mu Nang’ on a modified Murashige and Skoog (MS) medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration was achieved through organogenesis in callus cultures. Regenerated shoots were rooted on MS medium containing 0.2% activated charcoal but without plant growth regulators. Histological analysis revealed that calluses originated from small dense cells with well stained cytoplasm and nucleus typical of meristematic cells.

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Author Biographies

Kamnoon KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla, 90112
Assoc. Prof. Dr. Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Korn KORAPATCHAIKUL, Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla, 90112
Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Published
2012-02-22
How to Cite
KANCHANAPOOM, K., & KORAPATCHAIKUL, K. (2012). Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) ‘Kluai Sa’ and ‘Kluai Leb Mu Nang’. Notulae Scientia Biologicae, 4(1), 94-97. https://doi.org/10.15835/nsb417223
Section
Research articles
CITATION
DOI: 10.15835/nsb417223