Establishment of Cell Suspension Culture and Plant Regeneration in Abrus precatorius L., a Rare Medicinal Plant

  • Mohammad Serajur RAHMAN University of Rajshahi, Department of Genetic Engineering and Biotechnology, Rajshahi 6205
  • Mohammad Abdul Bari MIAH University of Rajshahi, Department of Genetic Engineering and Biotechnology, Rajshahi 6205
  • Mohammad Shahadat HOSSAIN University of Rajshahi, Department of Genetic Engineering and Biotechnology, Rajshahi 6205
  • Ahmad Humayan KABIR Flinders University, School of Biological Sciences, Bedford Park, SA 5042
  • Mohammad Motiur RAHMAN Flinders University, School of Biological Sciences, Bedford Park, SA 5042

Abstract

A new protocol has been developed for cell culture and in vitro regeneration of Abrus precatorius that holds enormous potentiality for preparation of medicines. In vitro grown calli were cultured in Murashige and Skoog (MS) liquid media in agitated condition fortified with 0.5 mg/l 6-Benzylaminopurine. Growth curve of cells revealed that the cells continued to grow until 12 days of culture and got the highest peak from day 6-8. Isolated cell was found to produce highest 8.2% calli when suspended on MS medium supplemented with 0.5 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. Callus derived from single cell produced highest number of embryo (25-28%) cultured on MS medium fortified with 2.0 mg/l 6-Benzylaminopurine and 0.2 mg/l 1-Naphthaleneacetic acid. The bipolar embryos were selected and optimum shoot formation was recorded on MS medium supplemented with 2.0 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. The optimum root induction was noticed in MS medium supplemented with 1.0 mg/l 3-Indolebutyric acid. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.

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Published
2012-02-22
How to Cite
RAHMAN, M. S., MIAH, M. A. B., HOSSAIN, M. S., KABIR, A. H., & RAHMAN, M. M. (2012). Establishment of Cell Suspension Culture and Plant Regeneration in Abrus precatorius L., a Rare Medicinal Plant. Notulae Scientia Biologicae, 4(1), 86-93. https://doi.org/10.15835/nsb417187
Section
Plant Sciences; Microbiology; Research Articles