Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026

Authors

  • Ahmad Humayan KABIR Flinders University, School of Biological Sciences, SA 5042
  • Anindya GHOSH ROY Max Planck Institute for Developmental Biology, D-72076 Tübingen
  • Mohammad FIROZ ALAM University of Rajshahi, Department of Botany, Rajshahi 6205, Bangladesh
  • Rafiul ISLAM University of Rajshahi, Department of Botany, Rajshahi 6205, Bangladesh

DOI:

https://doi.org/10.15835/nsb244863

Keywords:

quorum sensing, reporter strain, agar-plate based assay, bacterial communication

Abstract

Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate.

Author Biography

Ahmad Humayan KABIR, Flinders University, School of Biological Sciences, SA 5042

School of Biological Science

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Published

2010-12-05

How to Cite

KABIR, A. H., GHOSH ROY, A., FIROZ ALAM, M., & ISLAM, R. (2010). Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026. Notulae Scientia Biologicae, 2(4), 72–75. https://doi.org/10.15835/nsb244863

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Section

Research articles

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