In vitro regeneration of Haloxylon ammodendron

Ping WANG; Lingjuan MAN; Li MA; Jiaxin QI; Yanping REN; Zhengpei YAO; Bo WANG; Cong CHENG; Hua ZHANG

doi:10.55779/nsb15211585

Authors

Ping WANG Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Lingjuan MAN Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Li MA Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Jiaxin QI Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Yanping REN Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Zhengpei YAO Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Bo WANG Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Cong CHENG Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)
Hua ZHANG Xinjiang Agricultural University, College of Life Sciences, Urumqi, Xinjiang (CN)

DOI:

https://doi.org/10.55779/nsb15211585

Keywords:

adventitious buds, root induction, organogenesis, ex vivo regeneration, H. ammodendron

Abstract

Haloxylon ammodendron (C.A.Mey) Bunge is one of the important species of arid desert vegetation in China, and it is also an ideal material for studying plant stress resistance, which plays an extremely important role in protecting desert ecosystems and preventing land desertification. However, there are relatively few studies on the regeneration of the fusiform, and the genetic transformation system has not been successfully reported, which restricts the in-depth study of the molecular mechanism of shuttle propagation and stress resistance. In the present study, the seeds, hypocotyls, cotyledons, cotyledon nodes, terminal buds and fixed buds were used as explants, and a set of tissue culture and plant regeneration system was established by inducing adventitious buds, adventitious bud rooting and transplanting. The results showed that amongst different H. ammodendron explants, i.e., seeds, hypocotyls, cotyledons and cotyledon nodes, the last induced budding effect was better. The optimal medium for inducing clandine buds by cotyledon segment differentiation is 0.5 mg·L^-1 NAA+0.5 mg·L^-1 6-BA induced budding rate was high, reaching 61.90%, rooting medium was 1/2 MS+1 mg·L^-1 NAA +1 mg·L^-1 IBA + 1 mg·L^-1 IAA with a rooting rate of 50%. The results of this study will provide a theoretical basis for the genetic transformation of H. ammodendron.

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References

Amiri EM, Elahinia A (2011). Optimization of medium composition for apple rootstocks. African Journal of Biotechnology 10: 3594-3601. https://doi.org/10.4314/AJB.V10I18

Bertsouklis K, Paraskevopoulou AT & Petraki E (2023). In vitro regeneration from adult node explants of Juniperus oxycedrus. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 51(1):13062. https://doi.org/10.15835/nbha51113062

Binghua LIU, Yuanmao JIANG, Futian PENG (2008). Dynamic changes of endogenous hormone contents in the pulp and seeds of sweet cherry fruit during growth and development. Journal of Fruit Science. https://doi.org/10.13925/j.cnki.gsxb.2008.04.013

Casati P, Andreo CS, Edwards GE (1999). Characterization of NADP-malic enzyme from two species of Chenopodiaceae: Haloxylon persicum (C4) and Chenopodium album (C3). Phytochemistry 52(6):985-992. https://doi.org/10.1016/S0031-9422(99)00355-6

Chan Hoon An, Yong Wook Kim, Heung Kyu Moon, Jae Seon Y (2016). Effects of in vitro culture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipifera L.) from somatic embryos. Plant Biotechnology Journal 43(01):110-118. http://dx.doi.org/10.5010/JPB.2016.43.1.110

Dabauza M, Bordas M, Salvador A, Roig L-A, Moreno V (1997). Plant regeneration and Agrobacterium-mediated transformation of cotyledon explants of Citrullus colocynthis (L.) Schrad. Plant Cell Reports 16(12):888-892. https://doi.org/10.1007/s002990050340

Fernando SC, Goodger JQ, Gutierrez SS, Johnson AA, Woodrow IE (2016). Plant regeneration through indirect organogenesis and genetic transformation of Eucalyptus polybractea RT Baker. Industrial Crops and Products 86:73-78.https://doi.org/10.1016/j.indcrop.2016.03.025

Hu R, Sun Y, Wu B, Duan H, Zheng H, Hu D, ... Li Y (2017). Somatic embryogenesis of immature Cunninghamia lanceolata (Lamb.) hook zygotic embryos. Scientific Reports 7(1):1-14. https://doi.org/10.1038/s41598-017-00156-1

Jardak R, Boubakri H, Zemni H, Gandoura S, Mejri S, Mliki A, Ghorbel A (2020). Establishment of an in vitro regeneration system and genetic transformation of the Tunisian 'Maltese half-blood'(Citrus sinensis): an agro-economically important variety. 3 Biotech 10:1-11. https://doi.org/10.1007/s13205-020-2097-6

José García García, Eduardo Salas Alvarado, José Azofeifa Bolaños (2015). Effects of IAA and IBA on the in vitro rooting of stem cuttings of Sechium edule (Jacq.) Sw. Biotecnología Vegetal 15(01). https://revista.ibp.co.cu/index.php/BV/article/view/4/2

Kazuo T, Xiaoming Li, Kenji O (2000). Effects of sodium chloride on seed germination and growth of two Chinese desert shrubs, Haloxylon ammodendron and H. persicum (Chenopodiaceae). Australian Journal of Botany 48:455-460. https://doi.org/10.1071/BT99013

Kim YW, Moon HK (2007). Enhancement of somatic embryogenesis and plant regeneration in Japanese larch (Larix leptolepis). Plant Cell, Tissue and Organ Culture 88:241-245. https://doi.org/10.1007/s11816-014-0319-2

Kung H W, Chu G L, Tsien C P (1979). Flora: Reipubicae popularls sinicae. Beijing: Science Press in China 25(02):139-140.

Leshem B, Shaley DP, Izhar S (1988). Cytokinin as an inducer of vitrification in melon. Annals of Botany 61(02):255-260. https://doi.org/10.1093/oxfordjournals.aob.a087552

Li XY, Lv CQ, Huang BL, Wu QM, Zhang MH (2009). Adventitious roots induction of Pinus massoniana shoots in test tubes and anatomical observation. Journal of Northwest Forestry University 24(3), 80-84.

Maina JN, Wang Q (2015). Seasonal response of chlorophyll a/b ratio to stress in a typical desert species: Haloxylon ammodendron. Arid Land Research and Management 29:321-334. https://doi.org/10.1080/15324982.2014.980588

Miedema P (1984). The effects of growth regulators on vitrification in shoot cultures of Beta vulgaris. Acta Biochimica and Biophysica 33:375.

Miilion PM, Khandagale V, Nadkarin B (2015). Effect of IAA and IBA on in vitro rooting of banana (Musa paradisiaca) Cv. Grand Naine. International Journal of Engineering Science 4(05):959-962.

Pasqualetto PL, Zimmerman RH, Fordham I (1986). Gelling agent and growth regulator effects on shoot vitrification of 'Gala' apple in vitro. Journal of the American Society for Horticultural Science 111(6):976-980. https://doi.org/10.21273/JASHS.112.2.407

Su PX, Cheng GD, Yan QD, Liu XM (2007). Photosynthetic regulation of C4 desert plant Haloxylon ammodendron under drought stress. Plant Growth Regulation 51:139-147. https://doi.org/10.1007/s10725-006-9156-9

Wu X, Zheng XJ, Li Y, Xu GQ (2019). Varying responses of two Haloxylon species to extreme drought and groundwater depth. Environmental and Experimental Botany, 158, 63-72. https://doi.org/10.1016/j.envexpbot.2018.11.014

Xiaolan H E, Jingyin W U, Weimin Z, Qihan X (2001). Effects of 6BA and AgNO3 on adventitious shoot regeneration from the explants of cotyledon with petiole of rapeseed (Brassica napus L.). Jiangsu Journal of Agricultural Sciences.

Xu H, Li Y, Zou T, Xie J, Jiang L (2007). Ecophysiological response and morphological adjustment of H. ammodendron to-wards variation in summer precipitation. Acta Ecologica Sinica 27:5019-5028. https://doi.org/10.1111/j.1365-3040.2006.001626.x

Yasmin S, Nasiruddin KM, Begum R, Talukder SK (2003). Rengeneration and establinshment ofpotato plantlets through callus formation with BAP and NAA. Asian Journal of Plant Science 12(2):936-940. https://doi.org/10.3923/ajps.2003.936.940

Yu-Qing Z, Meng-Jie Z, Deng Z, Jun-Jie Z, Jing-Jian L, Xiao-Yang C (2018). In vitro plant regeneration of Zenia insignis Chun. Open Life Sciences 13(1):34-41. https://doi.org/10.1515/biol-2018-0005

Zhang X, Qin Y, Liang D, Zou Y, Ma F (2014). Enhancement of in vitro shoot regeneration from leaf explants of apple rootstock G. 41. In Vitro Cellular & Developmental Biology-Plant 50:263-270. https://doi.org/10.1007/s11627-013-9588-7