The Effect of Soil Mediated Genetic Changes and Plasmid Curing on Growth Rates of Pseudomonas and Staphylococcus spp. Isolated from Different Soils
Keywords:DNA isolation; pathogen evolution; plasmid; phytopathogen; virulence
The study was carried out to investigate the effect of soil mediated genetic change on growth rate of bacterial isolates from soils collected from different environments. Soil samples were collected from different sites: cow dung contaminated area, poultry, mechanic workshop, river, farmland and garden soils. Physiochemical analysis, bacteria isolation and enumeration were carried out on the different soil samples. Growth rate was determined by the measurement of turbidity at 600 nm using UV/VIS spectrophotometer on all isolates from two selected species (Pseudomonas and Staphylococcus) because of their even distribution in the soil samples. Plasmid curing was carried out on the selected isolates using 1 ml of 10% Sodium Dodecyl Sulphate as the curing agent and the growth rate of the plasmid-cured isolates were investigated. The results obtained indicated that the soil samples varied in their physiochemical properties. There were differences in the growth rates of bacterial isolates from the different soils for both Pseudomonas and Staphylococcus spp. a comparison between growth rate of test bacteria isolates before and after plasmid curing also suggested that curing significantly affected the growth rate of the test organisms in some soil samples. The results from this study suggested that the soil environments from which the various bacterial isolates were obtained had effect on the genetics of the bacteria isolates. The future direction to this study is to perform a molecular profiling of the various isolates to determine species specificity and also test for their virulence.
Adeyemo SM, Onilude AA (2015). Plasmid curing and its effect on the growth rate and physiological characteristics of Lactobacillus plantarum isolated form fermented cereals. Journal of Microbiology Research 5(1):11-22.
Agrios GN (2005). Plant pathology. Fifth Edition. Elsevier Academic Press, London.
Bennett MR, Pang WL, Ostroff NA, Baumgartner BL, Nayak S, Tsimring LS, Hasty J (2008). Metabolic gene regulation in a dynamically changing environment. Nature 454(7208):1119-1122.
Bremner DC, Mulvaney JM (1982). Total nitrogen. In: Page AL, Miller RH, Keaney DR (Eds). Methods of Soil Analysis. American Society of Agronomist. No 9 part 2.
Carattoli A (2011). Plasmids in Gram negatives: molecular typing of resistance plasmids. International Journal of Medical Microbiology 301(8):654-658.
Ehiaghe FA, Ehiaghe IJ, Agbonlahor DE, Oviasogie FE, Etikerentse SMO, Nwobu RAU, ... Enwa FO (2013). Plasmid profiling and curing analysis of fluoroquinolone multidrug resistant Pseudomonas aeruginosa in Benin City, Nigeria. Open Journal of Medical Microbiology 3(3):201-205.
Kalra YP, Maynard DG (1991). Methods manual for forest soil and plant analysis. Edmonton: Forestry Canada, Northwest region, Northern Forestry Center. Information Report NOR-X-319.
Kumar S (2011). Effects of soil physicochemical properties on Chlorpyrifos tolerant bacteria from cultivated soil. Electronic Journal of Environmental Sciences 4:17-23.
Onyeonwu RO (2000). Manual for waste/wastewater, soil/sediment, plant and fish analysis. McGill, Environmental Research Laboratory Manual, Benin City.
Ranjbar M, Karamian R, Tolui, Z, Amirabadizadeh H (2007). Onobrychis assadii (Fabaceae), a new species from Iran. Annales Botanici Fennici 44:481-484.
Sambrook JF, Russell DW (2001). Molecular cloning: a laboratory manual. Third edition. Cold Spring Harboar Laboratory Press.
Sengupta M, Austin S (2011). Prevalence and significance of plasmid maintenance functions in the virulence plasmids of pathogenic bacteria. Infection and Immunity 79(7):2502-2509.
Weaver RF (2012). Molecular biology. Fifth edition. McGraw-Hill Companies, New York, United State of America.
Zhang H, Hao Y, Zhang D, Luo Y (2011). Characterization of the cryptic plasmid pTXW from Lactobacillus paracasei TXW. Plasmid 65(1):1-7.
How to Cite
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.