TY - JOUR AU - BALILASHAKI, Khosro AU - GHASEMI GHEHSAREH, Masood PY - 2016/06/17 Y2 - 2024/03/29 TI - Micropropagation of Phalaenopsis amabilis var. ‘Manila’ by Leaves Obtained from in Vitro Culturing the Nodes of Flower Stalks JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 8 IS - 2 SE - Research articles DO - 10.15835/nsb829782 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/9782 SP - 164-169 AB - <p style="text-align: justify;">Orchids are one of the most popular plants in the world and among them the species of <em>Phalaenopsis</em> have the most sales on the global market. Because of its difficult propagation, micropropagation has been suggested recently. In the current study, the leaves obtained from <em>in vitro</em> culture of flower stalk nodes were used as explants and were cultured on MS medium with different concentrations of NAA, BA and TDZ. Protocorm-like bodies (PLBs) were produced and transferred to medium without growth regulators. Finally, the adaptation of plants was evaluated in a medium of cocopeat + coal (3:1 v/v) and another medium of cocopeat + charcoal + LECA (2:1:2 v/v). Results showed that the highest percentage of active samples was 100% which could regenerate the PLBs by being treated with 4 mg/l TDZ. The lowest active samples (60%) were those treated in the medium with 4 mg/l BAP + 0.5 mg/l NAA. The highest PLBs per explant (50.65) were obtained in the medium supplemented with 15 mg/l BAP + 3 mg/l NAA. Best acclimation (90%) of plants was obtained when medium of cocopeat + charcoal + LECA (2:1:2 v/v) was used. According to the results of the current experiment, the MS culture medium containing 15 mg/l BAP + 3 mg/l NAA was thereby considered as the best medium for <em>Phalaenopsis</em> micropropagation.</p> ER -