TY - JOUR AU - SHARMA, Gehamanjuri Laimayum AU - ATHOKPAM, Pinokiyo AU - THONGATABAM, Bijaya Devi PY - 2015/06/21 Y2 - 2024/03/29 TI - Molecular Characterization of Fusarium oxysporum G30 Isolated from Vegetable Wastes in Imphal, Northeast India JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 7 IS - 2 SE - Research articles DO - 10.15835/nsb729514 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/9514 SP - 184-187 AB - <p style="text-align: justify;">A new strain of <em>Fusarium oxysporum </em>(Schl.) emend. Synder &amp; Hansen, viz. <em>Fusarium oxysporum </em>G30, is reported from seven different decomposing vegetable wastes and a mixture of the same in equal proportions, in Imphal, Northeast India. This strain was isolated by surface sterilization technique, followed by re-culturing till pure culture was obtained. The pure culture was characterised morphologically based on the colony features: colour, texture, colony diameter; conidial features: macro and microconidial shape, size, septation, presence of chlamydospores and foot-shaped basal cells. The strain was also identified at molecular level by partial sequencing using universal primers, ITS4 and ITS5. The ITS data reveals that isolated culture (<em>F. oxysporum</em> G30) match the existing isolates in Gen Bank (Gen Bank accession No. GQ497156.1) by 99% and it has got a code segment particular to this strain. This strain showed ubiquitous nature with mean percent frequency of occurrence ranging from 39.5 to 68% at various stages of decomposition of the selected vegetable wastes.</p> ER -