TY - JOUR AU - UGWU, Ifeoma Chinyere AU - ANYANWU, Madubuike Umunna AU - UGWU, Chidozie Clifford AU - OKORO, Jude Chukwuemeka PY - 2015/03/20 Y2 - 2024/03/29 TI - Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 7 IS - 1 SE - Research articles DO - 10.15835/nsb719479 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/9479 SP - 20-25 AB - <p style="text-align: justify;">This study was conducted to isolate and detect methicillin-resistant staphylococci (MRS) in healthy broilers in Nsukka Southeast, Nigeria and determine the antibiogram of the isolates. Cloacal and skin swabs were collected from each of 101 randomly sampled broilers meant for slaughter. The samples were processed for isolation and identification of methicillin-resistant <em>Staphylococcus</em> species, following standard methods. Confirmation of methicillin-resistance by the isolates was done using penicillin binding protein 2a (PBP2a) kit. Phenotypic resistance of the isolates to antimicrobial agents was determined using disc diffusion method. Out of 202 samples processed, 200 (99.01%) yielded positive growth of staphylococci on oxacillin-supplemented oxacillin-resistance staphylococcal agar base (ORSAB). A total of 200 methicillin-resistant staphylococcal isolates were obtained. Of these, 91 (45.5%) were identified as methicillin-resistant coagulase-positive <em>Staphylococcus</em> (MRCoPS)<em>, </em>while 109 (54.5%) were identified as methicillin-resistant coagulase-negative <em>Staphylococcus</em> species (MRCoNS). Out of the 91 MRCoPS, 53 (58.2%) were identified as methicillin-resistant <em>Staphylococcus aureus</em> (MRSA). Resistance of the isolates was 99.5% to erythromycin and chloramphenicol, 100% to oxacillin, 76.5% to gentamicin, 96.5% to clindamycin, 92.5% to ciprofloxacin, 99% to sulphamethoxazole/trimethoprim and tetracycline, and 98.5% to streptomycin and cefoxitin. All the isolates were multidrug resistant. This study has shown that healthy broilers reared and slaughtered in Nsukka Southeast, Nigeria harbour multidrug-resistant MRS and thus serve as their reservoirs.</p> ER -