TY - JOUR AU - KABIR, Ahmad Humayan AU - GHOSH ROY, Anindya AU - FIROZ ALAM, Mohammad AU - ISLAM, Rafiul PY - 2010/12/05 Y2 - 2024/03/29 TI - Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 2 IS - 4 SE - Research articles DO - 10.15835/nsb244863 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/4863 SP - 72-75 AB - <p style="text-align: justify;">Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate.</p> ER -