TY - JOUR AU - DOUKKALI, Laaziza AU - TAHIRI, Abdessalem AU - ASKARNE, Latifa AU - TAZI, Bouchra AU - GUENOUN, Farhate AU - EZRARI, Said AU - LAHLALI, Rachid PY - 2022/05/30 Y2 - 2024/03/29 TI - Chemical composition and antibacterial activity of Moroccan Artemisia mesatlantica and A. absinthium essential oils against fire blight caused by Erwinia amylovora JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 14 IS - 2 SE - Research articles DO - 10.55779/nsb14211173 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/11173 SP - 11173 AB - <p>The aim of this study was to find an alternative to chemical products used in the control of fire blight disease of rosaceous plants. In this work, essential oils of two Moroccan Artemisia species:<em> Artemisia mesatlantica </em>and <em>Artemisia absinthium </em>were screened for their antibacterial activity against <em>Erwinia amylovora</em> the causal agent of this disease. The EOs extraction was carried out by water distillation method in Clevenger- type apparatus and analyzed by gas Chromatography-Flame Ionization Detector (GC/FID) and by Gas Chromatography-Mass Spectrometry (GC/MS). The antibacterial activity of extracted EOs was tested <em>in vitro</em> based on the agar diffusion method and broth microdilution method and <em>in vivo</em> on detached immature pear fruits. The results revealed that the yields of extracted EOs were 1.25 and 0.58% respectively for<em> A. mesatlantica </em>and <em>A. absinthium. </em>The chemical composition analysis showed that investigated EOs were characterized with a high amount of β-Thujone (40.42% for<em> A. mesatlantica </em>and 36.25% for<em> A. absinthium). </em>Both EOs have a similar MIC value (2.08%). <em>A. absinthium </em>EOs were found to be more effective than <em>A. mesatlantica</em> EOs since they show the lowest MBC. These results indicate that EOs of <em>A. absinthium </em>and <em>A. mesatlantica </em>contained compounds with antibacterial potential against <em>Erwinia amylovora</em>. In the bioassay test, the application of <em>A. absinthium </em>EOs on detached immature pear fruits infected by<em> E. amylovora</em> resulted in total suppression of disease severity. Such natural products may represent a sustainable alternative to the use of chemical pesticides.</p> ER -