TY - JOUR AU - MOHAMMAD, Anwar O. AU - ALKURTANY, Abdulkareem E.S. AU - HASSAN, Abdullah A. PY - 2020/06/29 Y2 - 2024/03/29 TI - Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 12 IS - 2 SE - Research articles DO - 10.15835/nsb12210628 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/10628 SP - 258-263 AB - <p style="text-align: justify;">Bacterial activity, mainly <em>Pseudomonas spp.</em> plays a vital role in the&nbsp;fruiting&nbsp;process of white button mushroom, hence a rapid procedure to identify these bacteria is crucial. In the current study, the validity of commercial identification system, Analytical profile index API 20E to identify <em>Pseudomonas</em> isolates from mushroom casing soil were assessed. Using API strips fifty bacterial isolates from a selective medium (King B medium) were examined, all isolates were belonged to the genus <em>Pseudomonas</em> according to API 20E identification systems. However, only 74% of <em>Pseudomonas</em> bacteria were identified to species level. The molecular identification using 16S rRNA gene was used as a reference tool to identify bacteria at the species level. The results show that the accuracy of the system to classify florescent <em>Pseudomonas</em> to species level was 60%. This was species dependant, and the system accuracy were 100%<em>,</em> 87.5%, 81.3% and 63% in identifying <em>P. aeruginosa</em>,<em> P. putida</em>, <em>P. fluorescens </em>and <em>P. tolaasii </em>respectively. Our finding indicates that although the classification of the <em>Pseudomonas</em> genus with API 20E system is useful, but it is not enough to distinguish these bacteria to species level, genomic studies are necessary to confirm the exact taxonomic position of <em>Pseudomonas </em>spp.</p> ER -