TY - JOUR AU - NAJEE, Haifaa Bawie AU - ALKURJIA, Dunya AU - ALMAHDAWY, Othman AU - KAMERZAN, Crina AU - MARUTESCU, Luminita AU - GHEORGHE, Irina AU - POPA, Marcela AU - CHIFIRIUC, Mariana Carmen AU - LAZĂR, Veronica PY - 2018/12/21 Y2 - 2024/03/29 TI - Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 10 IS - 4 SE - Research articles DO - 10.15835/nsb10410404 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/10404 SP - 498-502 AB - <p style="text-align: justify;">The aim of the present study was to investigate the potential antimicrobial activity of <em>Olea europaea</em> fatty oil against a collection of bacterial (<em>Staphylococcus aureus, Escherichia coli</em> and <em>Pseudomonas aeruginosa</em>) and fungal (<em>Candida albicans</em>) clinical isolates. The antimicrobial and antibiofilm activity were evaluated by broth microdilution method for establishing the minimal inhibitory concentration (MIC) and microtiter assay for determining the minimal biofilm eradication concentration (MBEC). Some of the potential microbial targets of the fatty oil were investigated by flow cytometry (FCM). The results obtained hereby revealed that <em>Olea europaea</em> fatty oil inhibited microbial planktonic growth (MICs values of 5.23-41.8 mg/ mL) and biofilm development on inert substrata (MBEC values of 1.31-20.9 mg/ mL). The FCM measurements confirmed that the analyzed oil induced microbial membrane damages and inhibited microbial efflux pump activity.</p> ER -