TY - JOUR AU - MISKSOKA-MILEVSKA, Elizabeta AU - POPOVSKI, Zoran T. AU - NESTOROVSKI, Tome PY - 2017/06/30 Y2 - 2024/03/29 TI - Usefulness of a Locus LE21085 in the Genetic Differentiation of Tomato Varieties JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 9 IS - 2 SE - Research articles DO - 10.15835/nsb9210114 UR - https://www.notulaebiologicae.ro/index.php/nsb/article/view/10114 SP - 209-213 AB - <p style="text-align: justify;">The microsatellites are specific for each individual genome or species. In order to evaluate the genetic diversity and the relationships within the genus <em>Lycopersicon</em>, microsatellites markers were used. The main objective of this study was to determine the usefulness of the locus LE21085 in the genetic differentiation among six morphologically different tomato varieties of <em>Lycopersicon esculentum</em> Mill. (var. <em>grandifolium</em> subsp. <em>cultum</em>; var. <em>cerasiforme - </em>red and yellow, var. <em>pruniforme,</em> var. <em>pyriforme</em> subsp. <em>subspontaneum</em> and var. <em>racemigerum</em> subsp. <em>spontaneum</em>). For the microsatellites locus LE21085 were detected two alleles in all estimated tomato varieties, that differed by one base pair (122 and 123 bp). The biggest allele frequency was found for the allelic variant of 122 bp, and its values were: 0.8462 for <em>L. esculentum</em> subsp. <em>subspontaneum </em>var. <em>cerasiforme</em> (red), 0.6923 for <em>L. esculentum</em> subsp. <em>subspontaneum </em>var. <em>cerasiforme</em> (yellow), 0.5769 for <em>L. esculentum</em> subsp. <em>cultum </em>var. <em>grandifolium</em>, 0.6923 for <em>L. esculentum</em> subsp. <em>subspontaneum</em> var. <em>pruniforme</em>, 0.6154 for <em>L. esculentum</em> subsp. <em>subspontaneum</em> var. <em>pyriforme</em> and 0.8077 for <em>L. esculentum</em> subsp. <em>spontaneum</em> var. <em>racemigerum</em>. The average of observed heterozygosity for the locus LE21085 (Ho = 0.5641) was higher than average expected heterozygosity (He = 0.4158). The average PIC value for the locus LE21085 was 0.3294 and it was classified as a modest informative markers. From the obtained results it can be concluded that locus LE21085 could be used in genetic differentiation of tomato varieties, but in combination with other polymorphic microsatellite loci.</p> ER -