@article{PRAMANIK_YUFDY_SHINTIAVIRA_WINARTO_2016, title={In Vitro Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of Phalaenopsis ‘AMP 17’}, volume={8}, url={https://www.notulaebiologicae.ro/index.php/nsb/article/view/9692}, DOI={10.15835/nsb819692}, abstractNote={<p style="text-align: justify;"><em>Phalaenopsis</em> ‘AMP 17’ is an important orchid commodity in Indonesia with high market demand; however, scaling up the orchid commercially is constrained by the availability and sustainability of qualified seedlings. To overcome the problem, a reliable <em>in vitro</em> propagation protocol, especially via secondary embryogenesis, was undertaken. In the present study, <em>in</em> <em>vitro</em> secondary embryogenesis derived from meta-topoline (mT) treatment on mass propagation of <em>Phalaenopsis</em> ‘AMP 17’ was successfully established. Embryos, as explant sources, were prepared by culturing meristem tips of flower stalk shoots on Murashige and Skoog (MS) medium containing 1.5 mg/L thidiazuron (TDZ) and 0.25 mg/L <em>N<sup>6</sup></em>-benzylaminopurine (BAP) for ± 3 months. High secondary somatic embryo (SSE) formation up to 64.90% with 12.30 SSEs regenerated per embryo was determined on half-strength MS augmented with 0.5 mg/L BAP and 2.5 mg/L mT. The combination also stimulated the result of high multiplication rate of SSE formation, up to 10.1 fold on the third subculture, maintained low conversion rate of germinated-embryos down to 55% and improved qualified-growth of the germinated embryos. The mT treatment produced 86% survival plantlets with high qualified-performance. The system could be applied as an alternative method to step forward towards an improved propagation protocol, commercially efficient due to high productivity. Detail findings in each step were discussed.</p>}, number={1}, journal={Notulae Scientia Biologicae}, author={PRAMANIK, Dewi and YUFDY, Muhammad PRAMA and SHINTIAVIRA, Herni and WINARTO, Budi}, year={2016}, month={Mar.}, pages={62–68} }