@article{PUNYARANI_SHARMA_2012, title={Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant}, volume={4}, url={https://www.notulaebiologicae.ro/index.php/nsb/article/view/7303}, DOI={10.15835/nsb427303}, abstractNote={<p style="text-align: justify;"><em>Costus pictus</em> D. Don is a potent anti-diabetic plant used in folk, ayurvedic and homeopathic system of medicine. Gene and protein expression of key targets in insulin signaling pathway have revealed that methyl tetracosonoate, a bio-active molecule from <em>Costus pictus</em> extract has anti-diabetic activity. The axillary buds of <em>Costus pictus</em> are dormant. The dormancy of axillary buds were broken when cultured in Murashige and Skoog (MS) medium supplemented with 3-4 μM 6-benzylaminopurine (BAP) in combination with 0.2-1 μM naphthalene acetic acid (NAA). The highest bud-break percentage was achieved in those supplemented with 0.6 μM NAA and 3 μM BAP. The sprouted axillary buds were transferred onto medium supplemented with 0.6 μM NAA and 6-10 μM BAP for shoot multiplication. The maximum average number of shoot was achieved in medium supplemented with 0.6 μM NAA and 8 μM BAP. The shoots were successfully rooted when transferred onto media supplemented with 1-12 μM NAA or indole-3-acetic acid (IAA) and 3 μM BAP. The maximum number of roots was found in 8 μM NAA and 3 μM BAP. The dormancy of <em>in vitro</em> axillary buds were also successfully broken in stems from which shoot apex were decapitated and cultured in MS medium with 0.6 μM NAA, 7 μM BAP and 5-13% sucrose. These sprouted <em>in vitro</em> axillary buds could be used as secondar y explants for shoot multiplication. The maximum was in medium supplemented with 9% sucrose. Rhizomes were successfully induced when 4-month old plantlets were cultured on ½ strength MS medium supplemented with 2.4 μM NAA, 32 μM BAP and 5-13% sucrose. Microrhizomes formed in 9% sucrose was largest in size with highest average fresh weight.</p>}, number={2}, journal={Notulae Scientia Biologicae}, author={PUNYARANI, Kshetrimayum and SHARMA, Jitendra G.}, year={2012}, month={May}, pages={72–78} }