Partial Purification and Characterisation of Alcohol Dehydrogenase from <i>Acetobacter aceti</i> Isolated from Palm Wine

  • Donatus Chimaobi ONAH University of Nigeria, Nsukka, Department of Biochemistry, Enugu State
  • Sabinus Oscar O. EZE University of Nigeria, Nsukka, Department of Biochemistry, Enugu State
  • Patrick Emeka ABA Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu state, Nigeria.

Abstract

Palm wine is a very important alcoholic beverage whose consumption is limited because it spoils easily. The study was designed to isolate Acetobacter aceti from palm wine, then extract, purify and characterize alcohol dehydrogenase (AD) from the A. aceti. Muller Hilton agar was used as medium for the growth of A. aceti for 48 h. The cells were harvested and subjected to ultrasonication using 500 watt ultrasonicator. Enzyme assay was carried out in both the supernatant and pellet. The enzyme was precipitated by polyethelene glycol 6000 while gel filtration was used for purifying the enzyme. The effects of pH, temperature and substrate concentration on AD were evaluated. The isolated A. aceti was gram negative, rod shaped, catalase positive, oxidase negative and was able to oxidize acetic acid to CO2 and H2O. Triton X-100 (0.3%) was the most effective concentration in solubilizing the protein (AD), while 15% polyethelene glycol 6000 was the most effective concentration for the precipitation of AD. An optimal pH of 5 was obtained with an optimal temperature of 50 °C. The most appropriate to solubilize and precipitate AD were 0.3% triton X-100 and 15% polyethelene glycol 6000 respectively, while AD activity was reduced under acidic pH, as well as for low and high temperatures.

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Author Biography

Patrick Emeka ABA, Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu state, Nigeria.

University of Nigeria, Nsukka, Department of Veterinary Physiology and Pharmacology, Enugu State

 

Published
2016-06-17
How to Cite
ONAH, D. C., EZE, S. O. O., & ABA, P. E. (2016). Partial Purification and Characterisation of Alcohol Dehydrogenase from <i>Acetobacter aceti</i&gt; Isolated from Palm Wine. Notulae Scientia Biologicae, 8(2), 232-236. https://doi.org/10.15835/nsb829810
Section
Research articles