In Vitro Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of Phalaenopsis ‘AMP 17’

  • Dewi PRAMANIK Indonesian Ornamental Crops Research Institute, Jln. Raya Ciherang, Pacet-Cianjur 43253, West Java-Indonesia (ID)
  • Muhammad PRAMA YUFDY Indonesian Center for Horticultural Research and Development, Jln. Raya Ragunan, No. 29A, Pasar Minggu, Jakarta Selatan 12040, Jakarta-Indonesia (ID)
  • Herni SHINTIAVIRA Indonesian Ornamental Crops Research Institute, Jln. Raya Ciherang, Pacet-Cianjur 43253, West Java-Indonesia (ID)
  • Budi WINARTO Indonesian Ornamental Crop Research Institute, Jln. Raya Ciherang, Pacet-Cianjur 43253, West Java, Indonesia (ID)
Keywords: hydroxylation benzyladenine; moth orchid; multiplication rate; repetitive embryogenesis; secondary somatic embryo; SSE

Abstract

Phalaenopsis ‘AMP 17’ is an important orchid commodity in Indonesia with high market demand; however, scaling up the orchid commercially is constrained by the availability and sustainability of qualified seedlings. To overcome the problem, a reliable in vitro propagation protocol, especially via secondary embryogenesis, was undertaken. In the present study, in vitro secondary embryogenesis derived from meta-topoline (mT) treatment on mass propagation of Phalaenopsis ‘AMP 17’ was successfully established. Embryos, as explant sources, were prepared by culturing meristem tips of flower stalk shoots on Murashige and Skoog (MS) medium containing 1.5 mg/L thidiazuron (TDZ) and 0.25 mg/L N6-benzylaminopurine (BAP) for ± 3 months. High secondary somatic embryo (SSE) formation up to 64.90% with 12.30 SSEs regenerated per embryo was determined on half-strength MS augmented with 0.5 mg/L BAP and 2.5 mg/L mT. The combination also stimulated the result of high multiplication rate of SSE formation, up to 10.1 fold on the third subculture, maintained low conversion rate of germinated-embryos down to 55% and improved qualified-growth of the germinated embryos. The mT treatment produced 86% survival plantlets with high qualified-performance. The system could be applied as an alternative method to step forward towards an improved propagation protocol, commercially efficient due to high productivity. Detail findings in each step were discussed.

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Published
2016-03-16
How to Cite
PRAMANIK, D., YUFDY, M. P., SHINTIAVIRA, H., & WINARTO, B. (2016). In Vitro Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of Phalaenopsis ‘AMP 17’. Notulae Scientia Biologicae, 8(1), 62-68. https://doi.org/10.15835/nsb819692
Section
Research articles
CITATION
DOI: 10.15835/nsb819692