Molecular Characterization of Fusarium oxysporum G30 Isolated from Vegetable Wastes in Imphal, Northeast India

  • Gehamanjuri Laimayum SHARMA Manipur University, Department of Life Sciences, Canchipur, Imphal-03 (IN)
  • Pinokiyo ATHOKPAM Dhanamanjuri College of Science, Department of Botany, Imphal-01 (IN)
  • Bijaya Devi THONGATABAM University of Mysore, Department of Studies in Zoology, Karnataka (IN)
Keywords: complex; decomposition; frequency of occurrence; Fusarium oxysporum species complex; microbial growth; new strain; vegetable waste management

Abstract

A new strain of Fusarium oxysporum (Schl.) emend. Synder & Hansen, viz. Fusarium oxysporum G30, is reported from seven different decomposing vegetable wastes and a mixture of the same in equal proportions, in Imphal, Northeast India. This strain was isolated by surface sterilization technique, followed by re-culturing till pure culture was obtained. The pure culture was characterised morphologically based on the colony features: colour, texture, colony diameter; conidial features: macro and microconidial shape, size, septation, presence of chlamydospores and foot-shaped basal cells. The strain was also identified at molecular level by partial sequencing using universal primers, ITS4 and ITS5. The ITS data reveals that isolated culture (F. oxysporum G30) match the existing isolates in Gen Bank (Gen Bank accession No. GQ497156.1) by 99% and it has got a code segment particular to this strain. This strain showed ubiquitous nature with mean percent frequency of occurrence ranging from 39.5 to 68% at various stages of decomposition of the selected vegetable wastes.

Metrics

Metrics Loading ...
Published
2015-06-21
How to Cite
SHARMA, G. L., ATHOKPAM, P., & THONGATABAM, B. D. (2015). Molecular Characterization of Fusarium oxysporum G30 Isolated from Vegetable Wastes in Imphal, Northeast India. Notulae Scientia Biologicae, 7(2), 184-187. https://doi.org/10.15835/nsb729514
Section
Research articles
CITATION
DOI: 10.15835/nsb729514