Callus Age and Size of Barley (Hordeum vulgare L.) Improves Regeneration Efficiency

  • Mozidul HAQUE Plant Genetic Engineering Lab, Institute of Biological Sciences, Third Science Building, University of Rajshahi, Rajshahi-6205
  • SM Shahinul ISLAM Plant Genetic Engineering Lab, Institute of Biological Sciences, Third Science Building, University of Rajshahi, Rajshahi-6205
Keywords: barley; callus age and weight; embryo size; in vitro; regeneration

Abstract

In order to improve regeneration efficiency embryos derived from immature seeds of BARI barley-6 were taken for this study. In this case callus size, age and its fresh weight were considered. Embryos sizes were classified into four groups: 0.6-1.0 mm (A), 1.1-1.5 mm (B), 1.6-2.0 mm (C) and 2.1-2.5 mm (D), and cultured to semi-solid MS medium for callus induction. Five weeks old calli were transferred to MS medium that contained 1.0 mg/l BAP + 150 mg/l L-glutamine, for regeneration. Results indicated that 1.6-2.0 mm size of embryos produced the highest callusing (56.72%) and green plantlets (42.16%), while small sized (0.6-1.0 mm) showed very poor (2.75%) callusing and no regeneration occurred. Calli were divided into three age groups e.g. early (1-3 weeks), medium (4-6 weeks) and prolonged age (7-12 weeks) and cultured to regeneration medium. To observe the effect of calli weight on regeneration, they were grouped into four categories: I (50-100 mg), II (101-150 mg), III (151-200 mg) and IV (>201 mg). The highest regeneration and rooting were recorded when the age of callus was 4-6 weeks and its weight range was 151–200 mg (III). The lowest regeneration and rooting were found when 1-3 weeks old calli were used and its average weight was 50-100 mg (I).

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Published
2015-06-21
How to Cite
HAQUE, M., & ISLAM, S. S. (2015). Callus Age and Size of Barley (Hordeum vulgare L.) Improves Regeneration Efficiency. Notulae Scientia Biologicae, 7(2), 188-191. https://doi.org/10.15835/nsb729497
Section
Research article