Simplified Regeneration Protocol for Cycas revoluta Thunb. Mature Zygotic Embryos

  • Rohangiz NADERI University of Tehran, Faculty of Agricultural Science and Engineering, Department of Horticulture, 31587-77871, Tehran (IR)
  • Khadije MOHAISENI University of Tehran, Faculty of Agricultural Science and Engineering, Department of Horticulture, 31587-77871, Tehran (IR)
  • Jaime A. TEIXEIRA DA SILVA P. O. Box 7, Miki-cho post office, Ikenobe 3011-2, Kagawa-ken, 761-0799 (JP)
  • Mansour OMIDI University of Tehran, Faculty of Agricultural Science and Engineering, Department of Agronomy and Plant Breeding, 31587-77871, Tehran (IR)
  • Behjat NADERI University of Tehran, Faculty of Agricultural Science and Engineering, Department of Agronomy and Plant Breeding, 31587-77871, Tehran (IR)
Keywords: 2,4-D; cycads; kinetin; mature zygotic embryo; plant growth regulator

Abstract

Mature zygotic embryos of Cycas revoluta Thunb. were used as explants to investigate direct and indirect organogenesis. Explants were incubated on half-strength Murashige and Skoog (½ MS) basal medium supplemented with various plant growth regulators, singly or in combination (all at 0.5 mg l-1): 6-benzyladenine (BA), kinetin (Kin), 2,4-dichlorophenoxyacetic acid (2,4-D), Kin×2,4-D, BA×Kin and BA×2,4-D. Cultures were placed at a low light intensity (4 μmol m-2 s-1 PPFD). Adventitious shoot regeneration was observed in the presence of 0.5 mg l-1BA after 35 days. The highest number of direct and indirect shoots per zygotic embryo was 3.67 and 29.67, respectively. Roots were induced on indirect shoots by continuous culture on rooting medium (½ MS,þ 0.1 mg l-1 1-naphthaleneacetic acid) and hardened successfully in perlite. Each rooted plantlet with pinnate leaves and a primary tap root was individually isolated and acclimatized 185 days after the beginning of culture, with a 10% success rate.

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Published
2015-03-20
How to Cite
NADERI, R., MOHAISENI, K., TEIXEIRA DA SILVA, J. A., OMIDI, M., & NADERI, B. (2015). Simplified Regeneration Protocol for Cycas revoluta Thunb. Mature Zygotic Embryos. Notulae Scientia Biologicae, 7(1), 62-65. https://doi.org/10.15835/nsb719482
Section
Research articles
CITATION
DOI: 10.15835/nsb719482