Optimization of Cultural Conditions for Production of Antibacterial Metabolites from Streptomyces coelicoflavus BC 01

  • Kothagorla Venkata RAGHAVA RAO Department of Biochemistry, Andhra University, Visakhapatnam 530 003, Andhra Pradesh (IN)
  • Dadi BHASKARA RAO Department of Biochemistry, Andhra University, Visakhapatnam 530 003, Andhra Pradesh (IN)
  • Botcha SATYANARAYANA Department of Biochemistry, Andhra University, Visakhapatnam 530 003, Andhra Pradesh (IN)
  • Tamanam RAGHAVA RAO Department of Biochemistry, Andhra University, Visakhapatnam 530 003, Andhra Pradesh (IN)
Keywords: optimization; antibacterial metabolites; cultural conditions; Streptomyces coelicoflavus; fermentation medium

Abstract

The aim of the present study was to optimize various cultural conditions for the production of antibacterial metabolites by Streptomyces coelicoflavus BC 01 isolated from mangrove soil, Visakhapatnam, Andhra Pradesh, India. The effect of various factors such as carbon and nitrogen sources, different concentrations of NaCl and K2HPO4, different temperature, pH, incubation time and agitation on antibacterial metabolites production were studied. The production of antibacterial metabolites by the isolate Streptomyces coelicoflavus BC 01 was greatly influenced by the cultural conditions. Glucose (1.2%) and soya bean meal (1%) seemed to be the best carbon and nitrogen source respectively, followed by NaCl (1%) and K2HPO4 (0.25%). Maximum production of antibacterial metabolites was observed at a temperature of 30 °C, with pH 7.2, at 160 rpm for 96 hrs. These optimized parameters can be further useful to design a fermentation medium to achieve maximum yield of antibacterial metabolites from Streptomyces coelicoflavus BC 01.

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Published
2015-06-21
How to Cite
RAGHAVA RAO, K. V., BHASKARA RAO, D., SATYANARAYANA, B., & RAGHAVA RAO, T. (2015). Optimization of Cultural Conditions for Production of Antibacterial Metabolites from Streptomyces coelicoflavus BC 01. Notulae Scientia Biologicae, 7(2), 151-159. https://doi.org/10.15835/nsb729481
Section
Research articles
CITATION
DOI: 10.15835/nsb729481