MtDNA Barcode Identification of Finfish Larvae from Vellar Estuary, Tamilnadu, India

  • Ramakrishnan THIRUMARAISELVI Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)
  • Sourin DAS Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)
  • Vellaichamy RAMANADEVI Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)
  • Muthusamy THANGARAJ Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)
Keywords: cytochrome oxydase I; genetic distance; ichthyoplankton; mitochondria

Abstract

Planktonic larvae were captured in the shallow Vellar estuary, Tamilnadu, India, during January to June 2013 using zooplankton net. Larvae were identified to the species level by comparison with Genbank data base using MtDNA sequence data. Further analysis showed that there is no sequence variation within-species, whereas between-species variation was more than 25%, supporting the suitability for species identification. The highest genetic distance was observed between A. nigropunctatus and M. cephalus (0.434) and the lowest genetic distance was observed between T. jarbua and S. commersoni (0.256). Given the current worldwide interest in DNA barcoding and species identification using MtDNA gene marker (CO1), it was confirmed the efficacy of the Fish-F1 and -R1 primer set, which gave the possibility for identification of finfish larvae at species level.

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Published
2015-03-20
How to Cite
THIRUMARAISELVI, R., DAS, S., RAMANADEVI, V., & THANGARAJ, M. (2015). MtDNA Barcode Identification of Finfish Larvae from Vellar Estuary, Tamilnadu, India. Notulae Scientia Biologicae, 7(1), 16-19. https://doi.org/10.15835/nsb719478
Section
Research articles
CITATION
DOI: 10.15835/nsb719478