Assessment of Field-Grown Cucurbit Crops and Weeds within Farms in South-West Nigeria for Viral Diseases

  • Emily Ibitaiyewa AYO-JOHN Federal University of Agriculture, Department of Crop Protection, PMB 2240, Alabata, Abeokuta, Ogun State, Nigeria; (NG)
  • Patience Mojibade OLORUNMAIYE Federal University of Agriculture, Department of Plant Physiology and Crop Production, PMB 2240, Alabata, Abeokuta, Ogun State, Nigeria (NG)
  • Olusola O. ODEDARA Federal University of Agriculture, Department of Microbiology, PMB 2240, Alabata, Abeokuta, Ogun State, Nigeria (NG)
  • Olusayo B. DADA Federal University of Agriculture, Department of Crop Protection, PMB 2240, Alabata, Abeokuta, Ogun State, Nigeria (NG)
  • Kolade O. ABIOLA Federal University of Agriculture, Department of Crop Protection, PMB 2240, Alabata, Abeokuta, Ogun State, Nigeria (NG)
  • John Oladeji OLADOKUN Federal University of Agriculture, Department of Crop Protection, PMB 2240, Alabata, Abeokuta, Ogun State, Nigeria (NG)
Keywords: Cucurbits; ELISA; pumpkin; survey; virus detection; watermelon

Abstract

Cucurbits are economic crops in Nigeria which serve as additional nutritional supplements and also good sources of income for farmers. Viral diseases are a worldwide problem of cucurbits and a major limiting factor for cucurbit production. A survey of farmer’s fields where cucurbit crops were grown was carried out to assess the incidence and severity of virus symptoms and viruses associated with the crops and weeds in selected locations in Ogun and Osun, in southwest Nigeria, in June, 2012. In all, 38 leaf samples were collected in Ogun state and 52 in Osun state from cucurbit crops and weeds. Leaf samples were tested against  Cucumber mosaic virus (CMV), Melon necrotic spot virus (MNSV), Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV),Zucchini yellow mosaic virus (ZYMV) and Cucumber green mottle mosaic virus (CGMMV) using Double Antibody Sandwich (DAS) enzyme-linked immunosorbent assay (ELISA). All the fields surveyed had virus symptom incidences of 100% except for melon fields in Osun state with incidences of between 10 and 30%. In Ogun state, the occurrence of CMV was 5/31 (16.1%) while MNSV was detected in Lagenaria siceraria and T. occidentalis and it occurred in 6.5% of the leaf samples. In Osun state, CMV was detected in watermelon, melon and weeds found in all locations surveyed. The occurrence of CMV was 9/38 (23.7%) in the cucurbit crops and in 78.6% (11/14) of the weeds. PRSV and WMV also occurred in mixed infection with CMV in 7.1% respectively. CMV was the most widespread and prevalent virus infecting cucurbit crops and weeds.Cucurbits are economic crops in Nigeria which serve as additional nutritional supplements and also good sources of income for farmers. Viral diseases are a worldwide problem of cucurbits and a major limiting factor for cucurbit production. A survey of farmer’s fields where cucurbit crops were grown was carried out to assess the incidence and severity of virus symptoms and viruses associated with the crops and weeds in selected locations in Ogun and Osun, in southwest Nigeria, in June, 2012. In all, 38 leaf samples were collected in Ogun state and 52 in Osun state from cucurbit crops and weeds. Leaf samples were tested against  Cucumber mosaic virus (CMV), Melon necrotic spot virus (MNSV), Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV) and Cucumber green mottle mosaic virus (CGMMV) using Double Antibody Sandwich (DAS) enzyme-linked immunosorbent assay (ELISA). All the fields surveyed had virus symptom incidences of 100% except for melon fields in Osun state with incidences of between 10 and 30%. In Ogun state, the occurrence of CMV was 5/31 (16.1%) while MNSV was detected in Lagenaria siceraria and T. occidentalis and it occurred in 6.5% of the leaf samples. In Osun state, CMV was detected in watermelon, melon and weeds found in all locations surveyed. The occurrence of CMV was 9/38 (23.7%) in the cucurbit crops and in 78.6% (11/14) of the weeds. PRSV and WMV also occurred in mixed infection with CMV in 7.1% respectively. CMV was the most widespread and prevalent virus infecting cucurbit crops and weeds.

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Published
2014-09-22
How to Cite
AYO-JOHN, E. I., OLORUNMAIYE, P. M., ODEDARA, O. O., DADA, O. B., ABIOLA, K. O., & OLADOKUN, J. O. (2014). Assessment of Field-Grown Cucurbit Crops and Weeds within Farms in South-West Nigeria for Viral Diseases. Notulae Scientia Biologicae, 6(3), 321-325. https://doi.org/10.15835/nsb639315
Section
Research articles
CITATION
DOI: 10.15835/nsb639315