In vitro Antibacterial Activity Analysis of Leaves of Limonia acidissima

  • Gandi Kishore NAIDU Department of Botany, Andhra University, Visakhapatnam (A.P.) 530 003 (IN)
  • B. SUJATHA Department of Botany, Andhra University, Visakhapatnam (A.P.) 530 003 (IN)
  • K. Chandra Sekhar NAIDU Department of Botany, Andhra University, Visakhapatnam (A.P.) 530 003 (IN)
Keywords: Antibacterial activity; Limonia acidissima; minimum inhibition concentration; methanol extract

Abstract

The present study was carried out the antibacterial activity and phytochemical screening of the hexane, chloroform and methanol extracts of leaves of Limonia acidissima. The antibacterial activity was evaluated against four Gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris) and five Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Staphylococcus aureus, Streptococcus pneumoniae) by agar well diffusion method. Methanol extract showed good antibacterial activity with the high inhibition zones while chloroform extract exhibited mild to moderate activity and hexane extract was found to be less active. Phytochemical screening revealed the presence of various secondary metabolites like steroids, alkaloids, phenols, flavonoids, coumarins, saponins, tannins and triterpenoids. The results of the present study suggest that leaves of Limonia acidissima can be used to treating infectious diseases caused by E. coli, P. vulgarisand S. pneumoniae.

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Published
2014-06-10
How to Cite
NAIDU, G. K., SUJATHA, B., & NAIDU, K. C. S. (2014). In vitro Antibacterial Activity Analysis of Leaves of Limonia acidissima. Notulae Scientia Biologicae, 6(2), 155-157. https://doi.org/10.15835/nsb629270
Section
Research articles
CITATION
DOI: 10.15835/nsb629270