Methods for Development of Microsatellite Markers: An Overview

  • Siju SENAN Indian Institute of Spices Research (IN)
  • Dhanya KIZHAKAYIL indian institute of spices research (IN)
  • Bhaskaran SASIKUMAR indian institute of spices research (IN)
  • Thotten Elampilay SHEEJA indian institute of spices research (IN)
Keywords: Chloroplast SSR; EST-SSR; Genetic diversity; Genomic SSR; Microsatellite isolation strategies; Simple Sequence Repeats


Microsatellite or Simple Sequence Repeat (SSR) markers have evolved to the status of a most versatile and popular genetic marker in a ubiquity of plant systems. Due to their co-dominant, hyper-variable and multiallelic nature, they are the prominent markers of choice for fingerprinting, conservation genetics, plant breeding and phylogenetic studies. Despite its development of a new set of SSR markers for a species remained time consuming and expensive for many years. However, with the recent advancement in genomics, new strategies/protocols are now available for the generation of SSR markers. This review presents an overview on microsatellite markers with a special emphasis on the various strategies used for the development of microsatellite markers.


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How to Cite
SENAN, S., KIZHAKAYIL, D., SASIKUMAR, B., & SHEEJA, T. E. (2014). Methods for Development of Microsatellite Markers: An Overview. Notulae Scientia Biologicae, 6(1), 1-13.
Review articles
DOI: 10.15835/nsb619199