Methods for Development of Microsatellite Markers: An Overview

Authors

  • Siju SENAN Indian Institute of Spices Research
  • Dhanya KIZHAKAYIL indian institute of spices research
  • Bhaskaran SASIKUMAR indian institute of spices research
  • Thotten Elampilay SHEEJA indian institute of spices research

DOI:

https://doi.org/10.15835/nsb619199

Keywords:

Chloroplast SSR; EST-SSR; Genetic diversity; Genomic SSR; Microsatellite isolation strategies; Simple Sequence Repeats

Abstract

Microsatellite or Simple Sequence Repeat (SSR) markers have evolved to the status of a most versatile and popular genetic marker in a ubiquity of plant systems. Due to their co-dominant, hyper-variable and multiallelic nature, they are the prominent markers of choice for fingerprinting, conservation genetics, plant breeding and phylogenetic studies. Despite its development of a new set of SSR markers for a species remained time consuming and expensive for many years. However, with the recent advancement in genomics, new strategies/protocols are now available for the generation of SSR markers. This review presents an overview on microsatellite markers with a special emphasis on the various strategies used for the development of microsatellite markers.

Downloads

Published

2014-03-12

How to Cite

SENAN, S., KIZHAKAYIL, D., SASIKUMAR, B., & SHEEJA, T. E. (2014). Methods for Development of Microsatellite Markers: An Overview. Notulae Scientia Biologicae, 6(1), 1–13. https://doi.org/10.15835/nsb619199

Issue

Section

Review articles