Molecular Identification and Phylogenetic Relationships of Threadfin Breams (Family: Nemipteridae) Using mtDNA Marker

  • Vaithilingam RAVITCHANDIRANE Kanchi Mamunivar Centre for Post-Graduate Studies, Department of Zoology, 605008 Pondicherry (IN)
  • Vaithianathan GEETHA Kanchi Mamunivar Centre for Post-Graduate Studies, Department of Zoology, 605008 Pondicherry (IN)
  • Vijayan RAMYA Kanchi Mamunivar Centre for Post-Graduate Studies, Department of Zoology, 605008 Pondicherry (IN)
  • Bilavendiran JANIFER Kanchi Mamunivar Centre for Post-Graduate Studies, Department of Zoology, 605008 Pondicherry (IN)
  • Muthusamy THANGARAJ Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamilnadu (IN)
  • Jayachandran SUBBURAJ Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamilnadu (IN)
  • Vellaichamy RAMANADEVI Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamilnadu (IN)
  • Takshnamurthy GANESAN Tagore Arts College, Department of Plant Science, 605008 Pondicherry (IN)

Abstract

Cytochrome c oxidase-1 gene sequences of mitochondrial genome were analyzed for species identification and phylogenetic relationship among the commercially important Nemipterus species. Sequence analysis of COI gene clearly indicated that all the nine fish species fell into distinct clads, which are genetically distant from each other and exhibited identical phylogenetic reservation. All the COI gene sequences provide sufficient phylogenetic information and evolutionary relationship to distinguish the nine Nemipterus species unambiguously. As per the neighbour-joining (NJ) and maximum likelihood (ML) trees, all the nine species are genetically distant from each other and exhibited identical phylogenetic reservation. Based on the NJ and ML phylogenetic trees N. mesoprion, N. zysron, N. hexodon, N. nematophorus, N. virgatus and N. bipunctatus were closely related with high bootstrap value (97). The overall mean Kimura two parameter (K2P) distances between the nine species was 0.109. The intra species K2P distance was high in N. japonicus (0.069) followed by N. peronii (0.050) and N. mesoprion (0.002). This study proves the use of mtDNA COI gene sequence based approach is an alternative tool for identifying fish species at a faster pace.

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Published
2012-05-10
How to Cite
RAVITCHANDIRANE, V., GEETHA, V., RAMYA, V., JANIFER, B., THANGARAJ, M., SUBBURAJ, J., RAMANADEVI, V., & GANESAN, T. (2012). Molecular Identification and Phylogenetic Relationships of Threadfin Breams (Family: Nemipteridae) Using mtDNA Marker. Notulae Scientia Biologicae, 4(2), 13-18. https://doi.org/10.15835/nsb427602
Section
Research articles
CITATION
DOI: 10.15835/nsb427602