In Vitro Clonal Propagation of a Fast Growing Legume Tree-Acacia mangium Willd. Employing Cotyledonary Node Explants
An efficient protocol for in vitro clonal propagation of A. mangium was developed using seedling derived explants. Out of three different explants tested for shoot proliferation, cotyledonary node showed best performance than leaf node and shoot tip explants. MS (Murashige and Skoog’s) medium was found best for shoot proliferation and cotyledonary nodes were subsequently cultured on MS medium supplemented with BA and Kn alone or in combination with NAA, IBA and GA3 at different concentrations. Maximum number of shoots was formed on MS medium containing 4.0 μM BA. For adventitious rooting, in vitro proliferated shoots were transferred to full strength MS medium fortified with IBA and NAA singly at different concentrations (0-8.0 μM). Best rooting responses were observed in the medium containing 8.0 μM IBA. Plantlets having well developed root system were transferred to soil and successfully acclimatized with 65% survival rate under ex vitro condition.
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.