In Vitro Clonal Propagation of a Fast Growing Legume Tree-Acacia mangium Willd. Employing Cotyledonary Node Explants
An efficient protocol for in vitro clonal propagation of A. mangium was developed using seedling derived explants. Out of three different explants tested for shoot proliferation, cotyledonary node showed best performance than leaf node and shoot tip explants. MS (Murashige and Skoog’s) medium was found best for shoot proliferation and cotyledonary nodes were subsequently cultured on MS medium supplemented with BA and Kn alone or in combination with NAA, IBA and GA3 at different concentrations. Maximum number of shoots was formed on MS medium containing 4.0 μM BA. For adventitious rooting, in vitro proliferated shoots were transferred to full strength MS medium fortified with IBA and NAA singly at different concentrations (0-8.0 μM). Best rooting responses were observed in the medium containing 8.0 μM IBA. Plantlets having well developed root system were transferred to soil and successfully acclimatized with 65% survival rate under ex vitro condition.
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