An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel

Abstract

The present study has an objective to optimize the extraction of Polyphenol Oxidase (PPO) from potato (Solanum tuberosum L.) peel. Response surface methodology (RSM) was used to design experiments and study the effect of six influential extraction parameters: extraction buffer concentration (100-500 mM), pH of extraction buffer (4.5-8.5), time (1-12 hours), temperature (4-40°C), concentration of PMSF (1-5 mM) and volume of extraction buffer (200-1000 ml) on the extraction of PPO. The dependent variable was considered as response function which was specific activity (SA) of extracted PPO. ANOVA was performed to obtain the regression equation that could predict the responses within given range. From RSM generated model, the optimum conditions for the maximum extraction of PPO were phosphate buffer concentration of 100 mm, buffer pH of 4.5, extraction time of 1 hour, 40°C temperature, PMSF concentration of 5 mM and buffer volume of 200 ml. Finally, this study illustrates a cost effective and less time consuming method to maximize the extraction of PPO from a vegetable waste.