A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum

  • Shasha WEI Shanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, Shanghai (CN)
  • Zhirui DENG Shanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, Shanghai (CN)
  • Liping YIN Shanghai Entry-Exit Quarantine and Inspection Bureau, 200135, Shanghai (CN)
  • Jianping YI Shanghai Entry-Exit Quarantine and Inspection Bureau, 200135, Shanghai (CN)
  • Renqi WU Shanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, Shanghai (CN)
  • Qin CHEN Shanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, Shanghai (CN)

Abstract

This work developed a novel nested polymerase chain reaction (n-PCR) assay to identify Sorghum nitidum (S. nitidum). It has been designed a set of specific n-PCR inner primers Snit5/Snit2 and outer primers Nout1/Nout2 based on a conserved nucleotide sequence of adh1-like gene of S. nitidum. Fourteen samples of sorghum were used to investigate the specificity of the primers and the n-PCR assay. The result showed that 9 samples of S. nitidum displayed a positive strong, specific amplified band at ~873 bp in gel spectra, while other relatives, including Sorghum halepense, Sorghum almum, Sorghum bicolor, Sorghum propinum and Sorghum sudanse exhibited negative amplifications. This assay was able to specifically identify S. nitidum fast and effectively, which could be applied widely in field inspection, agriculture production and plant protection.

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Published
2011-11-17
How to Cite
WEI, S., DENG, Z., YIN, L., YI, J., WU, R., & CHEN, Q. (2011). A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum. Notulae Scientia Biologicae, 3(4), 143-146. https://doi.org/10.15835/nsb346329
Section
Research articles
CITATION
DOI: 10.15835/nsb346329