The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

  • Naimeh SHARIFMOGHADAM Department of Agriculture Biotechnology, Science and Research Branch, Islamic Azad University, Tehran
  • Abbas SAFARNEJAD Razavi Khorasan Agricultural and Natural Resources Research Center. PO. Box. 91735-1148. Mashhad
  • Sayed Mohammad TABATABAEI Razavi Khorasan Agricultural and Natural Resources Research Center. PO. Box. 91735-1148. Mashhad

Abstract

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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Author Biographies

Naimeh SHARIFMOGHADAM, Department of Agriculture Biotechnology, Science and Research Branch, Islamic Azad University, Tehran
MSc of Agriculture Biotechnology
Abbas SAFARNEJAD, Razavi Khorasan Agricultural and Natural Resources Research Center. PO. Box. 91735-1148. Mashhad
Dept. of Natural Resources, Associate Prof.
Published
2011-08-25
How to Cite
SHARIFMOGHADAM, N., SAFARNEJAD, A., & TABATABAEI, S. M. (2011). The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis. Notulae Scientia Biologicae, 3(3), 97-100. https://doi.org/10.15835/nsb335931
Section
Plant Sciences; Microbiology; Research Articles

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