ROS Accumulation and TTC Reduction in Growing Embryo of Crithmum maritimum L. Isolated from Water or Salt Imbibed Seeds

  • Abdallah ATIA Laboratory of Extremophile Plants (LPE), Borj Cedria Centre of Biotechnology, Hammam-Lif, 2050 (TN)
  • Chedly ABDELLY Laboratory of Extremophile Plants (LPE), Borj Cedria Centre of Biotechnology, Hammam-Lif, 2050 (TN)
  • Abderrazak SMAOUI Laboratory of Extremophile Plants (LPE), Borj Cedria Centre of Biotechnology, Hammam-Lif, 2050 (TN)

Abstract

The salinity induced inhibition of seeds germination remains not clear at physiological levels. The aims of this study is to investigate the effect of salt on germination, embryo growth, superoxide anion radical (O2•–) and the respiratory activity (TTC reduction) in Crithmum maritimum L. seeds. Thus the embryo growth, in situ localization of respiratory activity and superoxide anion radical (O2•–) localization, were investigated. Chlorure 2, 3, 5-triphényltétrazolium (TTC) reduction test and superoxide anion radical (O2•–) localization with Nitroblue Tetrazolium Chloride (NBT) were performed in embryo isolated from seeds of the halophyte Crithmum maritimum L either sown in distilled water or in 200 mM NaCl. The key results show that germination was maximal (90 %) in distilled water, but was fully inhibited following seed exposure to NaCl. The completion of the embryo growth (ca. 2 mm length) leading to the radicle emergence took 6 d in H2O, but was markedly delayed by salt. NaCl reduced the elongation zone in the embryo axis, hence indicating that the cell division and/or cell elongation were disturbed by salinity. The respiratory activity (TTC reduction) and O2•– production in the cotyledon were significantly lowered by salinity.

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Author Biographies

Abdallah ATIA, Laboratory of Extremophile Plants (LPE), Borj Cedria Centre of Biotechnology, Hammam-Lif, 2050
Chedly ABDELLY, Laboratory of Extremophile Plants (LPE), Borj Cedria Centre of Biotechnology, Hammam-Lif, 2050
Abderrazak SMAOUI, Laboratory of Extremophile Plants (LPE), Borj Cedria Centre of Biotechnology, Hammam-Lif, 2050
Published
2011-05-16
How to Cite
ATIA, A., ABDELLY, C., & SMAOUI, A. (2011). ROS Accumulation and TTC Reduction in Growing Embryo of Crithmum maritimum L. Isolated from Water or Salt Imbibed Seeds. Notulae Scientia Biologicae, 3(2), 98-103. https://doi.org/10.15835/nsb325884
Section
Research articles
CITATION
DOI: 10.15835/nsb325884