Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers

  • Beáta ÁBRAHÁM Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.
  • Ildikó MIKLÓSSY Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.
  • Erika KOVÁCS
  • Éva TAMÁS
  • Ildikó MÉSZÁROS Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.
  • Szabolcs SZILVESZTER
  • Aurelia BREZEANU Institute of Biology Romanian Academy, Bucuresti, Spl. Independentei 296
  • Szabolcs LÁNYI Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.

Abstract

The purpose of the present study was to determine the level of genetic diversity within and among Ciuc basin, Romania (populations from Mohos and Luci raised bogs in Harghita Mountain and Sumuleu in Ciuc Mountain) Pinus sylvestris populations using molecular markers. Two of populations (Mohos and Luci) seems to be the descendants that survived the continental glaciation. Genetic diversity was analyzed by RAPD (Random Amplified Polymorphic DNA). Nine primers were selected for analysis, which generated reproducible bands. On base of presence or absence of homologues bands Nei’s gene diversity, the percentage of polymorphic loci and Nei’s unbiased genetic distance were calculated. The level of genetic variation among populations was found to be low. For both populations the variation values among populations were higher than within populations. The fossil records and geological historical data explain the extremely low genetic diversity of this species. Pinus sylvestris experienced strong bottlenecks during its evolutionary history, which caused the loss of genetic variation. Genetic drift and breeding in post-bottlenecked small populations may be the major forces that contribute to low genetic diversity and genetic differentiation of populations. Human activities may have accelerated the loss of genetic diversity in Pinus sylvestris.

 

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Author Biographies

Beáta ÁBRAHÁM, Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.
Ildikó MIKLÓSSY, Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.
Erika KOVÁCS
Éva TAMÁS
Ildikó MÉSZÁROS, Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.
Szabolcs SZILVESZTER
Aurelia BREZEANU, Institute of Biology Romanian Academy, Bucuresti, Spl. Independentei 296
Szabolcs LÁNYI, Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.
Published
2010-03-09
How to Cite
ÁBRAHÁM, B., MIKLÓSSY, I., KOVÁCS, E., TAMÁS, Éva, MÉSZÁROS, I., SZILVESZTER, S., BREZEANU, A., & LÁNYI, S. (2010). Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers. Notulae Scientia Biologicae, 2(1), 129-132. https://doi.org/10.15835/nsb213611
Section
Research articles