Integrated characterization and in vitro biocontrol of Fusarium sp. using Trichoderma asperellum

Authors

  • Sandra PÉREZ-ÁLVAREZ Universidad Autónoma de Chihuahua, Facultad de Ciencias Agrícolas y Forestales, Km 2.5 carretera a Rosales, Campus Delicias, C. P. 33000, Delicias, Chihuahua (MX)
  • César M. ESCOBEDO-BONILLA Instituto Politécnico Nacional-CIIDIR Unidad Sinaloa, México, Juan de Dios Bátiz Paredes No. 250, C.P. 81101 Guasave, Sinaloa (MX) https://orcid.org/0000-0001-8115-2476
  • Joel RASCÓN-SOLANO Universidad Autónoma de Chihuahua, Facultad de Ciencias Agrícolas y Forestales, Km 2.5 carretera a Rosales, Campus Delicias, C. P. 33000, Delicias, Chihuahua (MX) https://orcid.org/0000-0002-2541-4176
  • Samuel A. GARCÍA-GARCÍA Universidad Autónoma de Chihuahua, Facultad de Ciencias Agrícolas y Forestales, Km 2.5 carretera a Rosales, Campus Delicias, C. P. 33000, Delicias, Chihuahua (MX) https://orcid.org/0000-0001-5818-1391
  • Esteban SÁNCHEZ-CHÁVEZ Centro de Investigación en Alimentación y Desarrollo AC, Unidad Delicias, Cd. Delicias, Chihuahua C.P. 33089 (MX) https://orcid.org/0000-0002-6845-4290
  • Marco A. MAGALLANES-TAPIA Instituto Politécnico Nacional-CIIDIR Unidad Sinaloa, México, Juan de Dios Bátiz Paredes No. 250, C.P. 81101 Guasave, Sinaloa (MX) https://orcid.org/0000-0001-6340-1133
  • Héctor A. LEYVA HERNÁNDEZ Universidad Autónoma de Occidente, Departamento Académico de Ingeniería y Tecnología, Unidad Regional Los Mochis, Blvd. Macario Gaxiola, Los Mochis, Sinaloa C.P. 81217 (MX) https://orcid.org/0000-0002-9429-3478

DOI:

https://doi.org/10.55779/nsb16311997

Keywords:

antagonistic, biocontrol, Capsicum annuum L., fungal disease, Trichoderma

Abstract

Pepper is a crop of great economic importance in Mexico. However, it is affected by multiple diseases. The aim of this research was to identify through morphological and molecular methods the causal agent of both foliar and root wilting in jalapeño pepper plants (Capsicum annuum L.), and the ability of Trichoderma asperellum to control these diseases in vitro. The causal agent of the disease was isolated from soil and foliar samples of jalapeño pepper fields in Delicias, Chihuahua, Mexico. The fungus was cultured on solid PDA medium to study its morphology. The CTAB method was used to extract DNA from Fusarium sp. The ITS1 and ITS4 primer pair was used to amplify a 850 bp DNA fragment by PCR. The sequence was deposited in Genbank (acc. no. OR801070) and used for phylogenetic analysis using Bayesian inference. The analysis revealed five clades and the isolate was placed in clade 4, which showed high genetic similarity with F. oxysporum (99.28% identity). Based on these molecular, morphological and phylogenetic data, the isolate was identified as Fusarium oxysporum Schltdl. The biocontrol potential of T. asperellum (accession No. MN95047) against F. oxysporum was tested in vitro and it showed 40% to 87.5% inhibition of the pathogen. These findings are important for managing this pathogen and reducing the use of chemical fungicides.

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2024-09-24

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PÉREZ-ÁLVAREZ, S., ESCOBEDO-BONILLA, C. M., RASCÓN-SOLANO, J., GARCÍA-GARCÍA, S. A., SÁNCHEZ-CHÁVEZ, E., MAGALLANES-TAPIA, M. A., & LEYVA HERNÁNDEZ, H. A. (2024). Integrated characterization and in vitro biocontrol of Fusarium sp. using Trichoderma asperellum. Notulae Scientia Biologicae, 16(3), 11997. https://doi.org/10.55779/nsb16311997

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DOI: 10.55779/nsb16311997

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