Enhanced fig tree production through endomycorrhizal fungi inoculation: A nursery study on tree cuttings derived from the Ifrane Region, Morocco

Authors

  • Mouad BALLAOUI Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA)
  • Soumaya EL GABARDI Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA)
  • Manal ADNANI Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA) https://orcid.org/0000-0002-8988-5241
  • Karima SELMAOUI Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA)
  • Najia SAIDI Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA)
  • Soukaina MSAIRI Research Center of Plant and Microbial Biotechnologies, Biodiversity and Envirenment, Laboratory of Botany and Valorisation of Plant and Fungal Resources, Department of Biology, Faculty of Scinences, Mohammed V University in Rabat (MA)
  • Najoua MOUDEN Université Mohammed 1er Oujda, Faculté Pluridisciplinaire de Nador, Laboratoire de Chimie Moléculaire et Molécules de l’Environnement, FPN 300 Selouane, Nador (MA)
  • Moulay Abdelaziz EL ALAOUI Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA)
  • Amina OUAZZANI TOUHAMI Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA)
  • Allal DOUIRA Université Ibn Tofail, Faculté des Sciences, Département de Biologie, Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de Botanique, Biotechnologie et Protection des Plantes, Campus Universitaire, BP 242, Kenitra (MA)

DOI:

https://doi.org/10.55779/nsb16311972

Keywords:

arbuscular mycorrhizal fungi;, fig tree, inoculation, mycorrhizal association, Morocco

Abstract

The mycorrhizal association represents a crucial symbiotic bond necessary for the vitality of the majority of plants. This report aims to investigate the impact of endomycorrhizal fungi on the production of fig trees from cuttings. The research took place in a nursery environment by inoculating fig cuttings sourced from three sites in Morocco with a composite inoculum of arbuscular mycorrhizal fungi (AMF). The mean root and vegetative masses of the inoculated plants after 140 days were 33.1 g and 35 g compared with 1.8 g and 6.4 g in the control. The mean shoot length and number of leaves developed from treated cutting were 98.5 cm and 17, respectively 29 cm and 4 leaves in control. Thus, the newly formed roots showed a well-established mycorrhizal colonization and contained different structures characteristic of arbuscular endomycorrhizae: arbuscules, vesicles, endophytes, hyphae and spores. The frequency and intensity of root mycorrhization were approximately 80% and 15.5%, respectively. The contents of arbuscules and vesicles were 2.5% and 5.5%. The study of formed spores showed a combination of 52 spores per 100 g of soil, belonging to 11 species and 3 genera: Glomus, Acaulospora and Rhizophagus with dominance of the genus Glomus (85%). Glomus macrocarpum and Glomus deserticola were the most abundant species, appearing at 30.77% and 19.23%, respectively. The results demonstrate a pronounced stimulating effect on both root formation and vegetative shoot development in the inoculated cuttings. Specifically, the early inoculation of fig cutting with the AMF inoculum significantly enhanced rhizogenesis end root system development. Consequently, the gradual establishment of mycorrhizal symbiosis at the root level of inoculated plants led to good development of the root mass and the vegetative mass. The inoculated plants showed good root and vegetative mass development due to the installation and the progressive development of mycorrhizal symbiosis at their roots.

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2024-09-24

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BALLAOUI, M., EL GABARDI, S., ADNANI, M., SELMAOUI, K., SAIDI, N., MSAIRI, S., MOUDEN, N., EL ALAOUI, M. A., OUAZZANI TOUHAMI, A., & DOUIRA, A. (2024). Enhanced fig tree production through endomycorrhizal fungi inoculation: A nursery study on tree cuttings derived from the Ifrane Region, Morocco. Notulae Scientia Biologicae, 16(3), 11972. https://doi.org/10.55779/nsb16311972

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DOI: 10.55779/nsb16311972

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