Acute oral toxicity, antioxidant activity and total phenolic and flavonoid contents of Coronilla juncea
DOI:
https://doi.org/10.55779/nsb16411922Keywords:
antioxidants, Coronilla juncea, extracts, in vivo toxicity, polyphenolsAbstract
The aim of this work is the investigation of the in vivo toxicity, antioxidant activity, total phenolic and flavonoid contents of different parts of Coronilla juncea L. extracts (seeds, flowers, stems and roots). In the acute toxicity, the seeds extracts ethyl acetate (EtOAc) extract, median lethal dose (LD50) = 49.01 mg/kg was found to be highly toxic after oral administration in Swiss mice. The median lethal dose of other extracts petroleum ether (PE), n-butanol (n-BuOH) and residual aqueous ranged from 71.66 to 763.48 mg/kg and it was considered as moderately toxic. The antioxidant activity was evaluated using three different assays. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and phosphomolybdate assays, flowers exhibited a remarkable inhibitory effect (EtOAc extract half maximal inhibitory concentration (IC50) = 62.25 ± 0.35 and n-BuOH extract 80.23 ± 3.73 µg/mL) followed by stems extracts (n-BuOH extract IC50 = 71.03 ± 4.20 µg/mL) in DPPH assay and by seeds (n-BuOH extract IC50 = 88.03 ± 3.70 µg/mL) in phosphomolybdate assay. Compared with other parts of C. juncea, seeds extracts showed also the best generation of superoxide anion (EtOAc extract IC50 = 100.25 ± 1.55 µg/mL) followed by flowers (n-BuOH extract IC50 = 113.73 ± 3.89 µg/mL).To the best of our knowledge, this investigation reported for the first time the quantification of phenolic and flavonoid contents and assessed the antioxidant activity and in vivo toxic properties of four different parts of Coronilla juncea, indicating its potential application in therapeutic purposes.
Metrics
References
Ali-Snafi AE (2016). The pharmacological and toxicological effects of Coronillavaria and Coronillascorpiodes: A review. The Pharmaceutical and Chemical Journal 3(2):105-114. http://tpcj.org/download/vol-3-iss-3-2016/TPCJ2016-03-03-105-114.pdf
Alves CQ, David JM, David JP, Bahia MV, Aguiar RM (2010). Methods for determination of in vivo antioxidant activity for extracts and organic compounds. Quimica Nova 33(10):2202-2210. https://doi.org/10.1590/S0100-40422010001000033
Apak R, Capanoglu E, Shahidi F (2018). Measurement of antioxidant activity and capacity: recent trends and applications. John Wiley & Sons Ltd; first edition.
Bajaja YPS (1996). Biotechnology in agriculture and forestry: medicinal and aromatic plants IX. Springer-Verlage Berlin Heidelberg; first edition. https://link.springer.com/book/10.1007/978-3-642-59609-4
Beauchamp C, Fridovich I (1971). Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1):276-277. https://doi.org/10.1016/0003-2697(71)90370-8
Chanda S, Dudhatra S, Kaneria M (2010). Antioxidative and antibacterial effects of seeds and fruit rind of nutraceutical plants belonging to the Fabaceae family. Food & Fonction 1(3):308-315. https://doi.org/10.1039/c0fo00028k
Ferrante C, Angelini P, Venanzoni R, Flores GA, Tirillini B, Recinella L … Orlando G (2020). Antimicrobial, antioxidant, and antiproliferative effects of Coronilla minima: an unexplored botanical species. Antibiotics 9(9):611-626. https://doi.org/10.3390/antibiotics9090611
Hutchinson J (1964). The genera of flowering plants, Dicotyledons. Clarendon Press; Vol 1.
Innocenti G, Piovan A, Caniato R, Filippini R, Cappelletti EM (1996). Within-plant distribution of coumarins in Coronilla and Securigera species. International Journal of Pharmacognosy 34(2):114-118. https://doi.org/10.1076/phbi.34.2.114.13188
Khalil U, Mahmood T, Fatima I, Kanwal S (2022). Relative efficacy and toxicity studies on three wild medicinal plants of Fabaceae: a pharmaceutical perspective. Pakistan Journal of Botany 54(4):1567-1573. http://dx.doi.org/10.30848/PJB2022-4(34)
Khan RA, Khan MR, Sahreen S, Ahmed M (2012). Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chemistry Central Journal 6(1):43. https://doi.org/10.1186/1752-153X-6-43
Komissarenko NF, Zoz IG, Beletskii JN, Sokolow WS (1996). Zur chemotawonomischen charakterisierung von Coronilla scorpioides and Coronilla repanda. Planta Medica 17(2):170-117. https://doi.org/10.1055/s-0028-1099842
Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD (2007). Screening seeds of some Scottish plants for free-radical scavenging activity. Phytotherapy Research 21(7):615-621. https://doi.org/10.1002/ptr.2129
Lakache Z, Hacib H, Aliboudhar H, Toumi M, Mahdid M, Lamrani N, Tounsi H, Kameli A (2022). Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicity activities of the essential oil of Fortunella margarita peels. Journal of Biological Research 95(2):10641. https://doi.org/10.4081/jbr.2022.10641
Litchfield JT, Wilcoxon F (1949). A simplified method of evaluating dose-effect experiments. Journal of Pharmacology and Experimental Therapeutics 96(2):99-113.
Ganesan A (2008). The impact of natural products upon modern drug discovery. Current Opinion in Chemical Biology 12:306-317. https://doi.org/10.1016/j.cbpa.2008.03.016
Meyer AS, Isaksen A (1995). Application of enzymes as food antioxidants. Trends in Food Science & Technology 6(9):300-304. https://doi.org/10.1016/S0924-2244(00)89140-2
Uwakwa, Iwuanyanwu, Iheagwam (2014). Oral acute toxicity (LD50) study of methanol extract of Annona senegalensis leaf in albino mice. Sky Journal of Biochemistry Research 3:46-48. https://api.semanticscholar.org/CorpusID:39537012
Piovan A, Filippini R, Innocenti G (2014). Coumarin compounds in Coronilla scorpioides callus cultures. Natural Product Communications 9(4):489-492. http://dx.doi.org/10.1177/1934578X1400900415
Roblesw AB, Allegrettiz L, Passera CB (2002). Coronilla juncea is both a nutritive fodder shrub and useful in the rehabilitation of abandoned Mediterranean marginal farmland. Journal of Arid Environments 50(3):381-392. https://doi.org/10.1006/jare.2001.0913
Sato M, Ramarathnam N, Suzuki Y, Ohkubo T, Takeuchi M, Ochi H (1996). Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different sources. Journal of Agricultural and Food Chemistry 44(1):37-41. https://doi.org/10.1021/jf950190a
Sharififfar F, Dehghn-Nudeh G, Mirtajaldini M (2009). Major flavonids with antioxidant activity from Teucrium polium L. Food Chemistry 112:885-888. http://dx.doi.org/10.1016/j.foodchem.2008.06.064
Shraim AM, Ahmed TA, Rahman MDM, Hijji YM (2021). Determination of total flavonoid content by aluminium chloride assay: A critical evaluation. Food Science and Technology 150:111932-111943. https://doi.org/10.1016/j.lwt.2021.111932
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology 299:152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
Srinivasan S, Wankhar W, Rathinasamy S, Rajan R (2015). Free radical scavenging potential and HPTLC analysis of Indigofera tinctoria linn (Fabaceae). Journal of Pharmaceutical Analysis 6(2):125-131. https://doi.org/10.1016/j.jpha.2015.04.003
Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K (1994). A simple screening method for antioxidant and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Bioscience, Biotechnology, and Biochemistry 58(10):1780-1783. https://doi.org/10.1271/bbb.58.1780
Thomas G (2007). Medicinal chemistry. John Wiley & Sons; Second edition.
Umamaheswari M, Chatterjee TK (2007). In vitro antioxidant activities of the fraction of Coccinnia grandis L. leaf extract. African Journal of Traditional, Complementary and Alternative Medicines 5(1):61-73. https://pubmed.ncbi.nlm.nih.gov/20162057/
Vergun OM, Shymanska O, Valentyna F, Rakhmetov D (2020). DPPH free radical scavenging activity of some Fabaceae Lindl. species.
Agrobiodiversity for Improving Nutrition Health and Life Quality 4:124-133. https://doi.org/10.15414/agrobiodiversity.2020.2585-8246.124-133
Watson RR, Preedy VP (2019). Bioactive food as dietary interventions for diabetes. Elsevier Inc; second edition.
Zhishen J, Mengcheng T, Jianming M (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4):555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Hayat KHERKHACHE, Zina ALLAOUA, Mourad HANFER, Imane BENABDELAZIZ, Hamada HABA

This work is licensed under a Creative Commons Attribution 4.0 International License.
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
License:
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.