Protective effect of neem leaf extract and dexamethasone in cigarette-smoke induced liver and kidney injury in Swiss mice: A comparative analysis

Anushree VERMA; SUBHASHINI; Vishal SRIVASHTAV; Abhinav SINGH

doi:10.55779/nsb16111657

Authors

Anushree VERMA Banaras Hindu University, Plant Biotechnology, Rajiv Gandhi South Campus, Barkachha, Mirzapur, U.P. (IN)
SUBHASHINI Banaras Hindu University, Institute of Sciences, Department of Zoology, Varanasi, U.P. (IN)
Vishal SRIVASHTAV Banaras Hindu University, Plant Biotechnology, Rajiv Gandhi South Campus, Barkachha, Mirzapur, U.P. (IN)
Abhinav SINGH Banaras Hindu University, Institute of Agricultural Sciences, Department of Agricultural Statistics, Varanasi, U.P (IN)

DOI:

https://doi.org/10.55779/nsb16111657

Keywords:

Azadirachta indica, cigarette smoke, dexamethasone, kidney, liver, mice

Abstract

Cigarette smoke (CS) is one of the leading causes of lung injury where co-morbidity in different organs including liver and kidney can be observed. CS contributes as one of the causes of mortality across the globe. Dexamethasone, a corticosteroid is widely used as an anti-inflammatory medication despite being associated with several side effects with long-term usage. Therefore, the present study focused on the comparison between dexamethasone and Azadirachta indica leaf extract (AILE) for treating hepatic and renal injury caused by CS in mice. The phytochemical screening of AILE revealed the abundance of phytocompounds as sterols, proteins, alkaloids, flavonoids and diterpenes that might have antioxidant and anti-inflammatory activity. In this context, Swiss mice were treated with CS, dexamethasone and AILE. CS-induced mice expressed elevated inflammatory cells, Reactive Oxygen Species (ROS) with higher levels of both liver and kidney function markers: Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and creatinine respectively which was reversed by AILE. No mortality was observed in CS or AILE-treated group while dexamethasone-treated mice exclusively resulted in 20% mortality. Moreover, CS-induced mice also exhibited declined SOD levels in the liver and kidney and higher Eosinophil peroxidase (EPO) which was modulated by AILE treatment. This is the first report demonstrating in vivo the effects of A. indica ethanolic leaf extract in treating CS-induced hepatic and renal chronic inflammation in an animal model which gave better results when compared to dexamethasone.

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References

Adamko DJ, Wu Y, Gleich GJ, Lacy P, Moqbel R (2004). The induction of eosinophil peroxidase release: improved methods of measurement and stimulation. Journal of Immunological methods 291(1-2):101-108. https://doi.org/10.1016/j.jim.2004.05.003

Alsalhen KS, Abdalsalam AR (2014). Effect of cigarette smoking on liver functions: a comparative study conducted among smokers and non-smokers male in El-beida City, Libya. International Journal of Current Pharmaceutical Research 3(7):291-295. https://doi.org/10.3329/ICPJ.V3I7.19077

Balunas MJ, Kinghorn AD (2005). Drug discovery from medicinal plants. Life Sciences 78:431-441. https://doi.org/10.1016/j.lfs.2005.09.012

Benisheikh AAG, Muhammad FM, Kelluri H, Aliyu ZM, Mallam UB, Jibrin MW (2019). Phytochemical extraction and antimicrobial studies on crude leaf extract of Azadirachta indica (Neem) in semi-arid region of Borno state, Nigeria. International Journal of Current Research and Review 6(12):516-522. https://doi.org/10.15446/rcciquifa.v50n1.95447

Biswas K, Chattopadhyay I, Banerjee R, Bandhopadhyay U (2002). Biological activities and medicinal properties of neem (Azadirachta indica). Current Sciences 82(11):1336-1345.

Bojan N, Hemalatha D, Rangasamy B, Maharajan K, Ramesh M (2017). Hepatic oxidative stress, genotoxicity and histopathological alteration in fresh water fish Labeorohita exposed to organophosphorus pesticide profenofos. Biocatalysis and Agricultural Biotechnology 12:185-190. https://doi.org/10.1016/j.bcab.2017.09.006

Cichoż-Lach H, Michalak A (2014). Oxidative stress as a crucial factor in liver diseases. World Journal of Gastroenterology 20(25):8082-8091. https://doi.org/10.3748/wjg.v20.i25.8082

Das K, Samanta, Chainy GBN (2000). A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radicals. Indian Journal of Biochemistry and Biophysics 37:201-204. https://doi.org/10.4018/978-1-7998-3594-3.ch001

De S, Dey YN, Ghosh AK (2010). Phytochemical investigation and chromatographic evaluation of the different extracts of tuber of Amorphaphallus paeoniifolius (Araceae). International Journal of Biological & Pharmaceutical Research 1(5):150-157.

Demir S (2020). The process of acute and chronic inflammation: biomarkers and their relationship with diseases. Role of nutrition in providing pro-/anti-inflammatory balance: emerging research and opportunities. IGI Global, pp 1-23.

Eid A, Jaradat N, Elmarzugi N (2017). A review of chemical constituents and traditional usage of Neem plant (Azadirachta indica). Palestinian Medical & Pharmaceutical Journal 2:75-81.

El-Zayadi AR (2006). Heavy smoking and liver. World Journal of Gastroenterology 12(38):6098-6101. https://doi.org/10.3748/wjg.v12.i38.6098

Goorden SM, Buffart TE, Bakker A, Buijs MM (2013). Liver disorders in adults: ALT and AST. Nederlandse tijdschrift voor geneeskunde 157(43):A6443.

Gutteridge JMC, Halliwell B (1989). Iron toxicity and oxygen radicals. Bailliere's Clinical Haematology 2(2):195-256. https://doi.org/10.1016/s0950-3536(89)80017-4

Ijoma KI, Ajiwe VIE (2017). Phytochemical screening of Dialium indum leaf extract (Velvet tarmarind). International Journal of Phytopharmacy 7(1):06-13.

Kanagasanthosh K, Shanmugapriyan S, Kaviranjan V (2015). Evaluation of acute toxicity, anti-inflammatory activity and phytochemical screening of ethanolic extract of Azadirachta indica leaves. International Journal of Research and Development in Pharmacy & Life Sciences 4(5):1737-1742.

Karimi-Khouzani O, Heidarian E, Amini SA (2017). Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats. Pharmacological Reports 69(4):830-835. https://doi.org/10.1016/j.pharep.2017.03.011

Kumar RK (2015). Preliminary test of phytochemical screening of crude ethanolic and aqueous extract of Moringa pterygosperma Gaertn. Journal of Pharmacognosy and Phytochemistry 4(1):7-9.

Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y (2015). The role of oxidative stress and antioxidants in liver diseases. International Journal of Molecular Sciences 16(11):26087-26124. https://doi.org/10.3390/ijms161125942

Malkawi AK, Alzoubi KH, Jacob M, Matic G, Ali A, Al Faraj A, Abdel Rahman A M (2018). Metabolomics based profiling of dexamethasone side effects in rats. Frontiers in Pharmacology 9:46. https://doi.org/10.3389/fphar.2018.00046

Mohammed IMI (2019). Isolation, characterization of flavonoids from Terminalia brownie, Ziziphus abyssinica, Cassia sieberiana roots and evaluation of antimicrobial activity. Doctoral Dissertation, Sudan University of Science and Technology.

Nadhiya R, Sengottuvel T, Gopalasatheeskumar K, Ariharasivakumar G (2019). Phytochemical analysis and antioxidant activity of hydroalcoholic fruit extract of Cucumis dipsaceus. European Journal of Biomedical Research 6(12):281-286.

Ponist S, Zloh M, Bauerova K (2019). Impact of oxidative stress on inflammation in rheumatoid and adjuvant arthritis: damage to lipids, proteins, and enzymatic antioxidant defense in plasma and different tissues. Animal Models in Medicine and Biology, IntechOpen. https://doi.org/10.5772/intechopen.89480

Rady MY, Johnson DJ, Patel B, Larson J, Helmers R (2006) Corticosteroids influence the mortality and morbidity of acute critical illness. Critical Care 10(4):1-9. https://doi.org/10.1186/cc4971

Ragavan B, Krishnakumari S (2006). Effect of T. arjuna stem bark extract on histopathology of liver, kidney, and pancreas of alloxan-induced diabetic rats. African Journal of Biomedical Research 9:189-197. https://doi.org/10.4314/ajbr.v9i3.48904

Raja MMM, Raja A, Imran MM, Santha AMI, Devasena K (2011). Enzymes application in diagnostic prospects. Biotechnology 10(1):51-59. https://doi.org/10.3923/biotech.2011.51.59

Saidani C, Béchohra L, Laraba-Djebari F, Hammoudi-Triki D (2019). Kidney inflammation and tissue injury induced by scorpion venom: comparison with a nephrotoxic model. Toxin Reviews 38(3):240-247. https://doi.org/10.1080/15569543.2018.1446028

Saklani S, Mishra AP, Sati B, Sati H (2012). Pharmacognostic, phytochemical and antimicrobial screening of Aphanamixis polystachya, an endangered medicinal tree. International Journal of Pharmacy and Pharmaceutical Sciences 4(3):235-240.

Sati SC, Kumar P (2015). Assessment of Himalayan juniper, Juniperus squamata buch–hamex d. don for phytochemical screening and antimicrobial potential against some infection causing pathogens. World Journal of Pharmaceutical Research 4:998-1011.

Sen S, Chakraborty R (2017). Revival, modernization and integration of Indian traditional herbal medicine in clinical practice: Importance, challenges and future. Journal of Traditional and Complementary Medicine 7(2):234-244. https://doi.org/10.1016/j.jtcme.2016.05.006

Shahverdi MA, Omidi H, Tabatabaei SJ (2019). Stevia (Stevia rebaudiana Bertoni) responses to NaCl stress: Growth, photosynthetic pigments, diterpene glycosides and ion content in root and shoot. Journal of the Saudi Society of Agricultural Sciences 18(4):355-360. https://doi.org/10.1016/j.jssas.2017.12.001

Shin IS, Ahn KS, Shin NR, Lee HJ, Ryu HW, Kim JW, Oh SR (2016). Protective effect of EC-18, a synthetic monoacetyldiglyceride on lung inflammation in a murine model induced by cigarette smoke and lipopolysaccharide. International Immunopharmacology 30:62-68. https://doi.org/10.1016/j.intimp.2015.11.025

Sivakumar T, Gajalakshmi D (2013). Invitro antioxidant and chemical constituents from the leaves of Ormocarpum cochinchinensee lumbotti. American Journal of Plant Physiology 8(3):114-122. https://doi.org/10.3923/ajpp.2013.114.122

Sultana B, Anwar F, Przybylski R (2007). Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. Trees. Food Chemistry 104(3):1106-1114. https://doi.org/10.1016/j.foodchem.2007.01.019

Vinoth B, Manivasagaperumal R, Rajaravindran M (2012). Phytochemical analysis and antibacterial activity of Azadirachta indica A Juss. International Research Journal of Plant Science 2(3):50-55.

Waterborg JH, Matthews HR (1994). The Lowry method for protein quantitation. Methods Molecular Biology 32:1-4. https://doi.org/10.1385/0-89603-268-X:1

Yong SH, Leem AY, Kim YS, Park MS, Chang J, Kim SU, Jung JY (2020). Hepatic fibrosis assessed using fibrosis-4 index is predictive of all-cause mortality in patients with chronic obstructive pulmonary disease. International Journal of Chronic Obstructive Pulmonary Disease 15:831-839. https://doi.org/10.2147/COPD.S242863