Evaluation of reproduction biology of Prunus cerasoides

Suluh NORMASIWI; Siti S. HAFIZHAH; Ida ADVIANY; Muhammad I. SURYA

doi:10.55779/nsb15311601

Authors

Suluh NORMASIWI Research Center for Plant Conservation, Botanic Gardens and Forestry, National Research and Innovation Agency (BRIN), Cibinong 16911, West Java (ID)
Siti S. HAFIZHAH Universitas Islam Nusantara, Agriculture Faculty, Agrotechnology Department, Program of Agronomy, 40286 Bandung, West Java (ID)
Ida ADVIANY University of Islam Nusantara, Agriculture Faculty, Agrotechnology Department, 40286 Bandung, West Java (ID)
Muhammad I. SURYA Research Center for Plant Conservation, Botanic Gardens and Forestry, National Research and Innovation Agency (BRIN), Cibinong 16911, West Java (ID) https://orcid.org/0000-0003-3900-7376

DOI:

https://doi.org/10.55779/nsb15311601

Keywords:

anthesis, pollen, pollination, Prunus cerasoides, stigma, SEM

Abstract

Prunus cerasoides have a high value in phytochemistry and pharmacology. It was classified as the Least Concern globally based on the IUCN red list due to its widespread distribution in eastern Asia. This research aims to evaluate the reproductive biology of P. cerasoides through the study of pollen morphology, pollen viability, stigma receptivity, and pollination in the Cibodas Botanical Garden, located in the Cibodas subdistrict of West Java, Indonesia. The pollen morphology was observed using SEM. Moreover, the pollen viability test was followed by the staining method (aceto-orcein 2%, I₂KI 1%, TTC 1%) and in vitro pollen germination with thirteen treatments (aquadest [control]; 5-30% sucrose; and 5-30% sucrose + 5 ppm boric acid). Stigma receptivity was observed daily, from 7 days before anthesis until the anthesis stage. Furthermore, several types of pollination were evaluated, including open pollination, autogamy, geitonogamy, and allogamy. The results showed that the best staining method on P. cerasoides was aceto-orcein 2%, with pollen viability at 87.87%. The sucrose concentration of 25% at 72 hours of observation gave the highest pollen viability results, 52.48%. Stigma receptivity was optimal in the two days before anthesis until anthesis. The highest pollination efficiency was cross-pollination at 53.33%, with an average percentage of the total fruit set of 24.17%.

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