Insecticidal potential of Streptomyces sp. dichloromethane extracts against the cactus cochineal Dactylopius opuntiae (Cockerell)


  • Said RAMMALI Hassan First University of Settat, Faculty of Sciences and Techniques, Laboratory of Agro-Alimentary and Health, B.P. 539, Settat 26000 (MA)
  • Mohamed EL AALAOUI National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat principal, 10090 Rabat (MA)
  • Mohamed SBAGHI National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat principal, 10090 Rabat (MA)
  • Khadija DARI Hassan First University of Settat, Faculty of Sciences and Techniques, Laboratory of Agro-Alimentary and Health, B.P. 539, Settat 26000 (MA)
  • Bouchaib BENCHARKI Hassan First University of Settat, Faculty of Sciences and Techniques, Laboratory of Agro-Alimentary and Health, B.P. 539, Settat 26000 (MA)
  • Abdelhamid AZEROUAL Hassan First University of Settat, The Higher School of Education and Training of Berrechid, Laboratory of Agro-Alimentary and Health, B.P. 382, Poste Centrale, 26000 Settat (MA)
  • Abdelkrim KHATTABI Hassan First University of Settat, Faculty of Sciences and Techniques, Laboratory of Agro-Alimentary and Health, B.P. 539, Settat 26000 (MA)



Dactylopius opuntiae, dichloromethane extract, EP bacteria, IPM, Streptomyces sp.


Destructive damages caused by Dactylopius opuntiae (Cockerell) to cactus worldwide require an ecofriendly IPM approach. Streptomyces sp. produce wide range of biologically active secondary metabolites that can be an interesting alternative to chemical insecticides for pest control, as they are less toxic and easily biodegradable. The efficacy of dichloromethane extracts of four Moroccan Streptomyces sp. strains: Streptomyces bellus- E23-2, Streptomyces galilaeus- E23-9, Streptomyces africanus- E23-3, and Streptomyces bellus- E25-12 (applied at 11, 13, 15, 17 and 20 mg mL-1) against D. opuntiae nymphs and adult females was evaluated under laboratory and greenhouse conditions. Results showed that Streptomyces bellus- E23-2 and Streptomyces galilaeus- E23-9 dichloromethane extracts applied at 20 mg mL-1 were more effective, causing higher mortality against nymphs (92% and 91%, respectively) and adult females (90% and 95%, respectively) after 8 days of exposure, resulting in an LT50 value of 3.0 days (nymph), and 3.0 and 6.0 days (adult female), respectively. Streptomyces bellus- E25-12 extract had the lowest mortalities [88% (nymph) and 68% (adult female)]. In greenhouse experiment, the highest first instar nymph mortality was achieved by Streptomyces bellus- E23-2 (55.5%) and Streptomyces galilaeus- E23-9 (50.5%) dichloromethane extracts at 20 mg mL-1. The metabolites found in dichloromethane extracts of Streptomyces bellus- E23-2 and Streptomyces galilaeus- E23-9 show considerable potential to be used in the development of new biopesticide formulations for use in integrated pest management programs against D. opuntiae.


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How to Cite

RAMMALI, S., EL AALAOUI, M., SBAGHI, M., DARI, K., BENCHARKI, B., AZEROUAL, A., & KHATTABI, A. (2023). Insecticidal potential of Streptomyces sp. dichloromethane extracts against the cactus cochineal Dactylopius opuntiae (Cockerell). Notulae Scientia Biologicae, 15(4), 11574.



Research articles
DOI: 10.55779/nsb15411574