Elemental impact on antibacterial study of hydroalcoholic leaves extract of Belosynapsis vivipara

Authors

  • Kuntal DAS Mallige College of Pharmacy, Department of Pharmacognosy, #71, Silvepura, Chikkabanavara Post, Bangalore-560090 (IN)
  • Sravani SINGIRIKONDA Krupanidhi College of Pharmacy, Department of Pharmacognosy, #12/1, Chikkabelandur, Carmelaram Post, Varthur, Hobli, Bangalore-560035 (IN)

DOI:

https://doi.org/10.55779/nsb15111409

Keywords:

antibacterial studies, elemental analysis, Belosynapsis vivipara, MIC, MBC, microorganisms

Abstract

Belosynapsis vivipara (Dalzell) C.E.C. Fisch. (F: Commelinaceae) is one of the rare plant species located throughout Western Ghats regions including Karnataka. Though the plant was described earlier but traditional uses and scientific evidences are still lacking. The aim of the present study was to identify the elemental content and to determine antibacterial potentiality of Belosynapsis vivipara (Bv) ethanol leaves extract. Shade dried powdered material of BV leaves was estimated for elemental content using Atomic Absorption Spectrophotometer (AAS) followed by extraction by ethanol solvent (80%) in Soxhlet apparatus for 13 hrs at 45 °C. Extract was further used for anti-bacterial screening. In vitro antibacterial studies on the leaf extracts (25, 50 and 75 µg/ml) were carried out on medically important micro-organisms of Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa (Gram negative) and Staphylococcus aureus, Bacillus subtilis and Streptococcus pyogenes (Gram positive) against standard Ampicillin (25 µg/ml). The powdered drug showed the presence of zinc, and Copper in high amount less content of iron, whereas very less content of Nickel and Cobalt and absence of lead, mercury, arsenic and cadmium. The preliminary phytochemical screening revealed the presence of chemical constituents like alkaloids, flavonoids, phyto sterols, saponins and phenolics. Furthermore, ethanol leaves extract of BV showed broad spectrum antibacterial efficacy against both Gram positive and Gram-negative microorganism along with the dose dependency effects.  Antibacterial activity was correlated with the elements and showed positive correlation. Finally, Belosynapsis vivipara (Dalzell) C.E.C. Fisch. leaves were established as an effective source against strong bacterial infection.

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References

Abarca-Vargas R, Malacara CFP, Petricevich VL (2016). Characterization of chemical compounds with antioxidant and cytotoxic activities in bougainvillea x buttianaholttum and standl, (Var. rose) extracts. Antioxidants 5:45. https://doi.org/10.3390/antiox5040045

Aziz S, Saha K, Sultana N, Nur HP, Ahsan MA, Ahmed S, Hossain MK (2016). Comparative studies of elemental composition in leaves and flowers of Catharanthus roseus growing in Bangladesh. Asian Pacific Journal of Tropical Biomedicine 6(1):50-54. https://doi.org/10.1016/j.apjtb.2015.10.003

Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F, Omar AKM (2013). Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering 117(4):426-436. https://doi.org/10.1016/j.jfoodeng.2013.01.014

Betts JW, Wareham DW. (2014). In vitro activity of curcumin in combination with epigallocatechin gallate (EGCG) versus multidrug-resistant Acinetobacter baumannii. BMC Microbiology 14:172. https://doi.org/10.1186/1471-2180-14-172

Brown D (2015). Antibiotic resistance breakers: can repurposed drugs fill the antibiotic discovery void? Nature Reviews Drug Discovery 14(12):821-832. https://doi.org/10.1038/nrd4675

Das K, Tribedi S (2015). Effect of Zn, Fe and Cu content on phytochemical investigations and antimicrobial potential of Alternanthera brasiliana (L.) O. Kuntze leaf extracts procured from two different states of India. Turkish Journal of Pharmacological Sciences 12(3):345-356. https://doi.org/10.5505/tjps.2015.25733

Das K, Dang R, Sivaraman G, Ellath RP (2018). Phytochemical screening for various secondary metabolites, antioxidant, and anthelmintic activity of Coscinium fenestratum fruit pulp: a new biosource for novel drug discovery. Turkish Journal of Pharmacological Sciences 15(2):156-165. https://doi.org/10.4274/tjps.54376

Das K (2014). Phytochemical evaluation and comparative antibiocide efficacy of Aqueous, Ethanolic and equal mixture of aqueous and ethanolic (1:1) bark extract of Lannea coromandelica L. procured from Eastern region of India. International Letters of Natural Sciences 26:21-31. https://doi.org/10.56431/p-1eu719

Das K, Gupta NK, Sekeroglu N (2011). Studies on comparative antimicrobial potential of cultivated Patchouli oil and marketed Eucalyptus oil. International Journal of Natural and Engineering Sciences 5(3):1-7.

Devi KR, Subramani V, Nakulan VR, Annamalai P (2014). Qualitative and quantitative phytochemical analysis in four pteridophytes. International Journal Pharmaceutical Sciences 27(2):408-412.

El-Mesery HS, Mao H, El-Fatah Abomohra A (2019). Applications of non-destructive technologies for agricultural and food products quality inspection. Sensors 19:846. https://doi.org/10.3390/s19040846.

Fazly Bazzaz BS, Iranshahi M, Naderinasab M, Hajian S, Sabeti Z, Masumi E (2010). Evaluation of the effects of galbanic acid from Ferula szowitsiana and conferol from F. badrakema, as modulators of multi-drug resistance in clinical isolates of Escherichia coli and Staphylococcus aureus. Research in Pharmaceutical Sciences 5(1):21-28.

Fazly Bazzaz BS, Khameneh B, ZahedianOstad MR, Hosseinzadeh H (2018). In vitro evaluation of antibacterial activity of verbascoside, lemon verbena extract and caffeine in combination with gentamicin against drug-resistant Staphylococcus aureus and Escherichia coli clinical isolates. Avicenna Journal of Phytomedicine 8(3):246-253.

Hussein KN, Molnar TI, Pinter R, Toth A, Ayari E, Friedrich L, Dalmadi I, Kisko G (2020). In vitro antimicrobial activity of plant active components against Pseudomonas lundensis and Listeria monocytogenes. Progress in Agricultural Engineering Sciences 16:163-172. https://doi.org/10.1556/446.2020.20018

Jayaraman R (2009). Antibiotic resistance: an overview of mechanisms and a paradigm shift. Current Science India 96(11):1475-1484.

Kaur R, Jaiswal ML, Jain V (2013). Protective effect of Lannea coromandelica Houtt. Merill. against three common pathogens. Journal of Ayurveda and Integrative Medicine 4:224-228. https://doi.org/10.4103/0975-9476.123706

Kavade S, Deokule S, Lakshminarasimhan P, Diwakar P, Punekar S (2012). Belosynapsis vivipara (Dalzell) C.E.C. Fisch. (Commelinaceae), a vulnerable spiderwort, rediscovered after sixteen decades from Maharashtra, India. Journal of Threatened Taxa 4(6):2660-2663. https://doi.org/10.11609/JoTT.o2444.2660-3

Kheyrkhah M, Janmohammadi M, Abbasi A, Sabaghnia N (2018). The effects of micronutrients (Fe and Zn) and beneficial nano-scaled elements (Si and Ti) on some morphophysiological characteristics of oilseed rape hybrids. Agriculture (Poľnohospodárstvo) 64(3):116-127. https://doi.org/10.2478/agri-2018-0012

Knetsch MLW, Koole LH (2011). New strategies in the development of antimicrobial coatings: the example of increasing usage of silver and silver nanoparticles. Polymers Basel 3:340-366. https://doi.org/10.3390/polym3010340

Kowalska-Krochmal B, Dudek-Wicher R (2021). The minimum inhibitory concentration of antibiotics: methods, interpretation, clinical relevance. Pathogens 10(2):165. https://doi.org/10.3390/pathogens10020165

Kuete V, Wabo GF, Ngameni B, Mbaveng AT, Metuno R, Etoa FX, … Sokovic M (2008). Antioxidant and antimicrobial activity of Cynara cardunculus extracts. Food Chemistry 10:861-868. https://doi.org/10.1016/j.foodchem.2007.09.005

Liang J, Huang X, Ma G (2022). Antimicrobial activities and mechanisms of extract and components of herbs in East Asia. RSC Advances 12:29197-29213. https://doi.org/10.1039/D2RA02389J

Lo Piccolo E, Ceccanti C, Guidi L, Landi M (2021). Role of beneficial elements in plants: implications for the photosynthetic process. Photosynthetica 59(2):349-360. https://doi.org/10.32615/ps.2021.032

Meyer JJM, Lall N (2007). Antimicrobial activity of the methanolic extracts fractions and compounds from the stem bark of Irvingia gabonensis (Ixonanthaceae). Journal of Ethnopharmacology 114:54-60. https://doi.org/10.1016/j.jep.2007.07.025

Parham S, Kharazi AZ, Bakhsheshi-Rad HR, Nur H, Ismail AF, Sharif S, Ramakrishna S, Berto F (2020). Antioxidant, antimicrobial and antiviral properties of herbal materials. Antioxidants (Basel) 9(12):1309. https://doi.org/10.3390/antiox9121309

Poole K (2002). Mechanisms of bacterial biocide and antibiotic resistance. Journal of Applied Microbiology 92(suppl):55-64.

Sasane NA, Gaikar PS, Gaikwad SL, Valvi AK, Wadhawa GC (2021). Phytochemical analysis, anti-oxidant and anti-inflammatory of Belosynapsis vivipara leaves and roots. International Journal of Botany Studies 6(4):138-142.

Scheepmaker JW, Busschers M, Sundh I, Eilenberg J, Butt TM. (2019). Sense and nonsense of the secondary metabolites data requirements in the EU for beneficial microbial control agents. Biology Control 136:104005. https://doi.org/10.1016/j.biocontrol.2019.104005

Shakeri A, Sharifi MJ, Fazly Bazzaz BS, Emami A, Soheili V, Sahebkar A, Asili J (2018). Bioautography detection of antimicrobial compounds from the essential oil of salvia Pachystachys. Current Bioactive Compounds 14(1):80-85. https://doi.org/10.2174/1573407212666161014132503

Singh SK, Wu X, Shao C, Zhang H (2022). Microbial enhancement of plant nutrient acquisition. Stress Biology 2(3):1-14. https://doi.org/10.1007/s44154-021-00027-w

Tsuji BT, Yang JC, Forrest A, Kelchlin PA, Smith PF (2008). In vitro pharmacodynamics of novel rifamycin ABI-0043 against Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 62(1):156-160. https://doi.org/10.1093/jac/dkn133

Vandevelde NM, Tulkens PM, Van Bambeke F (2016). Modulating antibiotic activity towards respiratory bacterial pathogens by co-medications: a multi-target approach. Drug Discovery Today 21(7):1114-1129. https://doi.org/10.1016/j.drudis.2016.04.001

Published

2023-03-16

How to Cite

DAS, K., & SINGIRIKONDA, S. (2023). Elemental impact on antibacterial study of hydroalcoholic leaves extract of Belosynapsis vivipara . Notulae Scientia Biologicae, 15(1), 11409. https://doi.org/10.55779/nsb15111409

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Section

Research articles
CITATION
DOI: 10.55779/nsb15111409