Characterization of siderophore produced by Pseudomonas sp. MT and its antagonist activity against Fusarium oxysporum f. sp. cubense and F. oxysporium f. sp. ciceris

Authors

  • Mrugesh AMIN Charotar University of Science & Technology (CHARUSAT), PD Patel Institute of Applied Sciences, Changa - 388 421, Gujarat
  • Trupti K. VYAS Navsari Agricultural University, Food Quality Testing Laboratory, NM College of Agriculture, Navsari – 396450, Gujarat

DOI:

https://doi.org/10.55779/nsb14411298

Keywords:

biocontrol, Fusarium oxysporum, Pseudomonas, siderophore, PGPR

Abstract

Siderophores are low molecular weight iron scavengers produced by bacteria to combat iron stress and also suppress deleterious rhizobacteria. In the present study, microbes were isolated from wheat and tobacco farm in Changa village, Anand district, India, and were screened for their siderophore production. Out of 11 isolates, 6 were siderophore producers as they produced orange halos on CAS agar. Isolated bacteria were examined for their hydroxamate, catechol, and carboxylate type of siderophore, and it revealed that all produced hydroxamate siderophore. Among all the isolates, a potential bacterium was selected for further studies and identified by the biochemical test as Pseudomonas sp. MT. Temporal effect on growth and siderophore production revealed that both were higher at 24 hrs of incubation and remained active up to 8 days and then after the decline. Siderophore was partially purified and chemically characterized by FTIR. A particle size analyzer measured the particle size of the siderophore and showed 91.36 nm in size. The siderophore producer and non-producer were examined for their ability to uptake iron by providing external siderophores, which gave positive results. The isolated bacterium was tested for its antagonistic activity against Fusarium oxysporum f. sp. cubense and F. oxysporium f. sp. ciceris, resulting in inhibition of both the species. Hence, Pseudomonas sp. MT can be effectively used to control Fusarium spp.

References

Arnow LE (1937). Colorimetric determination of the components of 3, 4-dihydroxyphenylalanine–tyrosine mixtures. Journal of Biological Chemistry 118:531-537. https://doi.org/10.1016/S0021-9258(18)74509-2

Buysens S, Heungens K, Poppe J, Höfte M (1996). Involvement of pyochelin and pyoverdin in suppression of Pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7NSK2. Applied and Environmental Microbiology 62:865-871. https://doi.org/10.1128/AEM.62.3.865-871.1996

Champomier-Vergès MC, Stintzi A, Meyer JM (1996). Acquisition of iron by the non-siderophore-producing Pseudomonas fragi. Microbiology 142(5):1191-1199. https://doi.org/10.1099/13500872-142-5-1191

Chiriani L, Tobacchioni S, Bevivino A (1993). Interactions between rhizosphere microorganisms under iron limitation. Archives of Microbiology 160:68-73.

Cohen JI, Falconi C, Komen J (1998). Strategic decisions for agricultural biotechnology. Synthesis of four policy seminars. 38:1-11.

Fekete FA, Chandhoke V, Jellison J (1989). Iron-binding compounds produced by wood-decaying basidiomycetes. Applied and Environmental Microbiology 55:2720-2722. https://doi.org/10.1128/aem.55.10.2720-2722.1989

Ghazy N, El-Nahrawy S (2021). Siderophore production by Bacillus subtilis MF497446 and Pseudomonas koreensis MG209738 and their efficacy in controlling Cephalosporium maydis in maize plant. Archives of Microbiology 203:1195-1209 https://doi.org/10.1007/s00203-020-02113-5

Jadhav RS, Desai AJ (1992). Isolation and characterization of siderophore from cowpea Rhizobium (peanut isolate). Current Microbiology 24:137-141 https://doi.org/10.1007/BF01568978

Joshi G, Khan A, Akolkar A, Pandya A, Archana G, Desai A (2006). Differential cross-utilization of heterologous siderophores by nodule bacteria of Cajanus Cajan and its possible role in growth under iron-limited conditions. Applied Soil Ecology 34:19-26. https://doi.org/10.1016/j.apsoil.2005.12.001

Lurthy T, Cantat C, Jeudy C, Declerck P, Gallardo K, Barraud C, Leroy F, Ourry A, Lemanceau P, Salon C, Mazurier S (2020). Impact of bacterial siderophores on iron status and ionome in pea. Frontiers in Plant Science 11:730. https://doi.org/10.3389/fpls.2020.00730

Molina AB, Valmayor RV (1999). Banana production systems in South East Asia. In: Pica C, Foure E, Frison EA (Eds). Bananas and Food Security. INIBAP, Montpellier, France, pp 423-436.

Neilands JB (1981). Microbial iron transport compounds (siderophores) as chelating agents. In: Martell AE, Anderson WF, Badman DG (Eds). Development of Iron Chelators for Clinical Use. Elsevier, Amsterdam pp 13-31.

NHB (2020). National Horticulture Board.

Parveen SR, Latha D (2019) Characterization of siderophore producing Pseudomonas sp for its plant growth promoting properties. Bioscience Biotechnology Research Communications 12(4). http://dx.doi.org/10.21786/bbrc/12.4/26

Pérez-Miranda S, Cabirol N, George-Téllez R, Zamudio-Rivera LS, Fernández FJ (2007). O-CAS, a fast and universal method for siderophore detection. Journal of Microbiological Methods 70(1):127-31. http://dx.doi.org/10.1016/j.mimet.2007.03.023

Ploetz RC (2015). Fusarium wilt of banana. Phytopathology 105:1512-1521. https://doi.org/10.1094/PHYTO-04-15-0101-RVW

Ploetz RC, Pegg KG (1997). Fusarium wilt of banana and Wallace’s line: Was the disease originally restricted to his Indo-Malayan region? Australasian Plant Pathology 26:239-249. https://doi.org/10.1071/AP97039

Schiessl KT, Janssen EM-L, Kraemer SM, McNeill K, Ackermann M (2017). Magnitude and mechanism of siderophore-mediated competition at low iron solubility in the Pseudomonas aeruginosa pyochelin system. Frontiers in Microbiology 8:1964. https://doi.org/10.3389/fmicb.2017.01964

Schwyn B, Neilands JB (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160:47- 56. https://doi.org/10.1016/0003-2697(87)90612-9

Shenker M, Oliver I, Helmann M, Hadar Y, Chen Y (1992). Utilization by tomatoes of iron mediated by a siderophore produced by Rhizopus arrhizus. Applied and Environmental Microbiology 15:2173-2182. https://doi.org/10.1080/01904169209364466

Shin SH, Lim Y, Lee SE, Yang NW, Rhee JH (2001). CAS agar diffusion assay for the measurement of siderophores in biological fluids. Journal of Microbiological Methods 44:89-95. https://doi.org/10.1016/S0167-7012(00)00229-3

Snow GA (1954). Mycobactin. A growth factor for Mycobacterium johnei. Part II. Degradation, and identification of fragments. Journal of the Chemical Society 49:2588-2596 https://doi.org/10.1039/JR9540004080

Stover RH (1962). Fusarial wilt (Panama Disease) of bananas and other Musa species. Commonwealth Mycological Institute, Kew, England.

Trivier D, Dravril M, Houndret N, Courcol RJ (1995). Influence of iron depletion on growth kinetics, siderophore production and protein expression of Staphylococcus aureus. FEMS Microbiology Letters 127:195-199. https://doi.org/10.1111/j.1574-6968.1995.tb07473.x

Vadnerker PS, Vyas TK, Kapadia C, Gandhi A (2018) Multifaceted plant growth promoting potentials of Pseudomonas aeruginosa AP isolated from Dandi, Gujarat, India. International Journal of Chemical Studies 6(6):1146-1151.

Verma C, Dixit A, Kumar R (2019). Production, optimization, and characterization of siderophore by Pseudomonas aeruginosa (C3) isolated from rhizospheric Soil. In: Kundu R, Narula R (Eds). Advances in Plant & Microbial Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-13-6321-4_5

Wardlaw CW (1961). Banana Diseases: Including Plantains and Abaca. Longmans, London.

Published

2022-11-28

How to Cite

AMIN, M., & VYAS, T. K. (2022). Characterization of siderophore produced by Pseudomonas sp. MT and its antagonist activity against Fusarium oxysporum f. sp. cubense and F. oxysporium f. sp. ciceris. Notulae Scientia Biologicae, 14(4), 11298. https://doi.org/10.55779/nsb14411298

Issue

Section

Research articles