Parquetina nigrescens leaf infusion: a food-based approach for the management of diet-induced iron deficiency in weanling rats

Keywords: anaemia, infusion, iron deficiency, micronutrient, Parquetina nigrescens

Abstract

Micronutrients are vitamins and minerals needed by the body in minute amount and whose impact on the body’s health is vital. Hence, deficiency in any one of them could cause severe and even life-threatening conditions. Deficiency in iron is the most common micronutrient deficiency and is prevalent in children and pregnant women. The infused extracts of Parquetina nigrescens (PN) leaf were used in the management of iron deficiency in weanling rats to provide a food-based approach strategy that is readily accessible, affordable, and acceptable to the low-income population who are more susceptible to micronutrient deficiencies. Diet-induced iron-deficient weanling rats (55.0 ± 10.0) g was administered infused extracts (PN, PN+ ginger PN+ lemongrass, PN+ milk) of PN at 1000 mg/kg body weight for 28 days. The haemoglobin concentration of rats administered PN extract was significantly (p < 0.05) increased compared to the control. Serum protein assay indicated administration of PN extract reduced significantly (p < 0.05) concentrations of Divalent Metal Transporter 1, transferrin, ferroportin, and increased hepcidin and ferritin concentrations. Higher concentrations of flavonoids in the PN extract are proposed to support its haematopoietic activity alongside its ascorbic acid content. The infused extract of PN showed better haematopoietic activity when taken alone than when additives were added.

Metrics

Metrics Loading ...

References

Abbaspour N, Hurrell R, Kelishadi R (2014). Review on iron and its importance for human health. Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences 19(2):164-174.

Adeyemi SB, Abidakun O, Azeez RO, Chijindu PCI, Oyebanji OO (2018). Phytochemical and nutritional composition of commonly used medicinal plants for the treatment of anaemia in Kwara State, Nigeria. Annals of West University of Timişoara 21(2):165-174.

Agbor AG, Odetola AA (2001). Hematological studies of Parquetina nigrescens on haemorrhagic anaemic rats. African Journal of Medicine and Medical Sciences 30(1-2):105-109.

Agbor GA, Odetola AA (2005). Effect of Parquetina nigrescense on erythrocyte indices and serum electrolyte of rats following acute blood loss. Pakistan Journal of Biological Science 8(4):527. https://doi.org/10.3923/pjbs.2005.527.531

Awoniyi AJ (2019). Anti-anaemic activity of Parquetina nigrescens leaf supplementation in iron deficient weanling and pregnant rats. Ph.D. Thesis, University of Ilorin, Ilorin, Nigeria.

Barisani D, Parafioriti A, Bardella MT, Zoller H, Conte D, Armiraglio E, ... Weiss G (2004). Adaptive changes of duodenal iron transport proteins in celiac disease. Physiological Genomics 17:316-325. https://doi.org/10.1152/physiolgenomics.00211.2003

Clark SF (2008). Iron deficiency anaemia. Nutrition in Clinical Practice 23(2):128-141. https://doi.org/10.1177/0884533608314536

Cook JD, Reddy MB (2001). Effect of ascorbic acid intake on non-haem iron absorption from a complete diet. American Journal of Clinical Nutrition 73:93-98. https://doi.org/10.1093/ajcn/73.1.93

Delaby C, Pilard N, Gonalves AS, Beaumont C, Canonne-Hergaux F (2005). Presence of the iron exporter ferroportin at the plasma membrane of macrophages is enhanced by iron loading and down-regulated by hepcidin. Blood 106(12):3979-3984. https://doi.org/10.1182/blood-2005-06-2398

Fermandes MIM, Galvao LC, Bortolozzi MF, Oliveira WP, Zucoloto S, Bianchi MLP (1997). Disaccharidase levels in normal epithelium of the small intestine of rats with iron-deficient anaemia. Brazilian Journal of Medical Biological Research 30:849-854. https://doi.org/10.1590/s0100-879x1997000700005

FAO (Food and Agricultural Organization of the United Nations). (2021). The state of food security and nutrition in the world. Transforming food systems for food security, improved nutrition and affordable healthy diet for all. https://doi.org/10.4060/cb4474en

FAO (Food and Agriculture Organization of the United Nations). (2013). The state of food and agriculture. Rome. http://bit.ly/KAn84P

Frazer DM, Anderson GJ (2014). The regulation of iron transport. BioFactors 40(2):206-214. https://doi.org/10.1002/biof.1148

Ganz T (2005). Hepcidin-a regulator of intestinal iron absorption and iron recycling by macrophages. Best Practice and Research Clinical Haematology 18:171-182. https://doi.org/10.1016/j.beha.2004.08.020

Harborne JB (1998). Phytochemical methods: A guide to modern techniques of plant analysis. Chapman &Hall, London, pp 279.

Iyare EE, Obaji NN (2014). Effects of aqueous leaf extract of Azadirachta indica on some haematologicalparameters and blood glucose level in female rats. Nigerian Journal of Experimental and Clinical Biosciences 2(1):54-58.

Iyare EE, Ezeh OC, Nubila T, Orizu AI (2015). Effects of Hibiscus sabdariffal consumption during pregnancy on biochemical parameters and litter birth weight in rats. African Journal of Traditional Complementary and Alternative Medicine 12(5):79-83.

Jagadish LK, Krishnan VV, Shenbhagaraman R, Kaviyarasan V (2009). Comparative study on the antioxidant, anticancer and antimicrobial property of Agaricusbisporus imbach before and after boiling. African Journal of Biotechnology 8:654-661.

Kayode OT, Yakubu MT (2017). Parquetina nigrescens leaves: chemical profile and influence on the physical and biochemical indices of sexual activity of male Wistar rats. Journal of Integrative Medicine 15(1):64-76. https://doi.org/10.1016/S2095-4964(17)60318-2

Kelly AU, McSorley ST, Patel P, Talwar D (2017). Interpreting Iron Studies. British Medical Journal 357:1-6. https://doi.org/10.1136/bmj.j2513

Kumari S, Gupta A (2016). Nutritional composition of dehydrated Ashwagandha, shatavari and ginger root powder. International Journal of Home Science 2(3):68-70.

Kyaw A (1978) A simple colorimetric method for ascorbic acid determination in blood plasma. Clinica et Chimica Acta 86:153-157. https://doi.org/10.1016/0009-8981(78)90128-6

Lanzkowsky G, Karayalcin F, Miller F (1982). Disaccharidase levels in iron deficient rats at birth and during nursing and post weaning periods: response to iron treatment. Pediatric Research 16(4):318-323. https://doi.org/10.1203/00006450-198204000-00016

Makanjuola OY, Dada OE, Akharaiyi FC (2010). Antibacterial potentials of Parquetina nigrescens extracts on some selected pathogenic bacteria. Journal of Natural Products 3:124-129.

Modupe O, Oladiji TA (2016). Optimizing dose of aqueous extract of Magnifera indica Lstem bark for treating anaemia and its effect on some disaccharidases activity in iron deficient wealning rats. Journal of Nutrition and Intermediary Metabolism 3(2016):18-22. https://doi.org/10.1016/j.jnim.2015.12.001

Nagababu E, Gulyani S, Christopher J, Earley CJ, Cutler RY, ... Rifkind JM (2008). Iron-deficiency anaemia enhances red blood cell oxidative stress. Free Radical Research 42(9):824-829. https://doi.org/10.1080/10715760802459879

Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM (2004). Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306:2090-2093. https://doi.org/10.1126/science.1104742

Obadoni BO, Ochuko PO (2001). Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Sciences 8:203-208. https://doi.org/10.4314/gjpas.v8i2.16033

Odetola AA, Oluwole FS, Adeniyi BA, Olatiregun AM, Ikupolowo OR, Labode O (2006). Antimicrobial and gastrointestinal protective properties of Parquetina nigrescens (Afzel.) Bullock. Journal of Biological Science 6(4). https://doi.org/10.3923/jbs.2006.701.705

Oladiji, AT, Jacob, TO, Yakubu, MT (2007). Anti-anaemic potentials of aqueous Sorghum bicolor (L.) moench stem bark in rats. Journal of Ethnopharmacology 111(3):651-656. https://doi.org/10.1016/j.jep.2007.01.013

Olajire A, Azeez L (2011). Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. African Journal of Food Science and Technology 2(2):022-029.

Owoyele BV, Oyelowo OT, Biliaminu SA, Alaran ON, Alimi SA, Saliu RS (2011). Hematological and biochemical studies on Parquetina nigrescens root extract in albino rats. Journal of Applied Pharmaceutical Science 1(10):176-179.

Raja SB, Murali MR, Kumar NK, Devaraj SN (2011). Isolation and partial characterisation of a novel lectin from Aegle marmelos fruit and its effect on adherence and invasion of shigella to HT29 cells. PloS One 6(1):1-9. https://doi.org/10.1371/journal.pone.0016231

Saha U, Dharwadkar PS, Sur S, VishahariniV, Malleshappa M (2018). Plant extracts as an astounding remedy to anemia-A review. Annals of Plant Sciences 7(4):2166-2171. https://doi.org/10.21746/aps.2018.7.4.16.

Sharp PA (2010). Intestinal iron absorption: Regulation by dietary and systemic factors. International Journal for Vitamin and Nutrition Research 80:231-242. https://doi.org/10.1024/0300-9831/a000029

Shawki A, Anthony SR, Nose Y, Engevik MA, Niespodzany EJ, Barrientos T, … Mackenzie B (2015). Intestinal DMT1 is critical for iron absorption in the mouse but is not required for the absorption of copper or manganese. American Journal of Physiology, Gastrointestinal and Liver Physiology 309(8):G635-G647. https://doi.org/10.1152/ajpgi.00160.2015

Sopeyin AO, Ajayi GO (2016). Pharmacognostic study of Parquetina nigrescens (Afz el.) Bullock (Periplocaceae). International Journal of Phamacognosy and Phytochemical Research 8(2):321-326.

Teucherl B, Olivares M, Cori H (2004). Enhancers of iron absorption: ascorbic acid and other organic acids. International Journal for Vitamin and Nutrition Research 74(6):403-419. https://doi.org/10.1024/03009831.74.6.403

Trease GE, Evans WC (1989). A Textbook of Pharmacognosy. 13th ed. Bailliere-Tindall Ltd., London.

Vlachodimitropoulos E, Naftalin RJ, Sharp PA (2010). Quercertin is a substrate for the transmembrane oxidoreductase Dcytb. Free Radical Biology and Medicine 48(10):1366-1369. https://doi.org/10.1016/j.freeradbiomed.2010.02.021

WHO (World Health Organization) (2008). World Prevalence of Anaemia 1993-2005 WHO Global Database on Anaemia. Geneva: World Health Organization.

WHO (World Health Organization) (2011). Serum ferritin concentrations for the assessment of iron status and iron deficiency in populations. https://apps.who.int/iris/handle/10665/85843

Yakubu MT, Akanji MA, Oladiji AT (2005). Aphrodisiac potentials of aqueous extract of Fadogia agrestis (Schweinf. Ex Heirn) stem in male albino rats. Asian Journal of Andrology 7:399-404. https://doi.org/10.1111/j.1745-7262.2005.00052.x

Published
2022-09-23
How to Cite
AWONIYI, A. J., ABIDAKUN, O. M., & OLADIJI, A. T. (2022). Parquetina nigrescens leaf infusion: a food-based approach for the management of diet-induced iron deficiency in weanling rats. Notulae Scientia Biologicae, 14(3), 11275. https://doi.org/10.55779/nsb14311275
Section
Research articles
CITATION
DOI: 10.55779/nsb14311275