Hypoglycemic and in vitro antioxidant activities of Stereospermum kunthianum stem bark hydromethanol extract

  • Julius O. ODE University of Abuja, Department of Veterinary Pharmacology & Toxicology, PMB 117 Abuja (NG)
  • Florence NWINYI University of Abuja, Department of Veterinary Pharmacology & Toxicology, PMB 117 Abuja (NG)
  • Maxwell O. EGUA University of Abuja, Department of Pharmacology and Therapeutics, PMB 117 Abuja (NG)
  • Monday M. ONAKPA University of Abuja, Department of Veterinary Pharmacology & Toxicology, PMB 117 Abuja (NG)
  • Ganiyat M. AKANDE University of Abuja, Department of Veterinary Pharmacology & Toxicology, PMB 117 Abuja (NG)
  • Samuel O. ONOJA Michael Okpara University of Agriculture, Department of Veterinary Physiology and Pharmacology, Umudike, PMB 7267, Abia State (NG)
  • Hudu G. MIKAIL University of Abuja, Department of Veterinary Pharmacology & Toxicology, PMB 117 Abuja (NG)
  • David D. AKUMKA University of Abuja, Department of Veterinary Pharmacology & Toxicology, PMB 117 Abuja (NG)
  • Mohammed ADAMU University of Abuja, Department of Veterinary Pharmacology & Toxicology, PMB 117 Abuja (NG)
Keywords: Diabetic indices, extraction, glibenclamide, hyperglycemia, oxygen radicals

Abstract

This study investigated Stereospermum kunthianum stem bark hydromethanol extract for hypoglycemic and in vitro antioxidant activities. Phytochemical analyses were performed to determine the components of the extract.  In vitro antioxidant activities of the extract were assessed with 1, 1-diphenyl-2-picrylhydrazyl radical scavenging and ferric reducing /antioxidant power assays. An oral acute toxicity study to determine the safe dose of the extract was conducted in albino Wistar rats. Evaluation of the extract's hypoglycemic activities on blood glucose levels of normal, glucose loaded and streptozotocin-induced diabetic rats were performed. The extract contained various constituents, including saponins, terpenes, tannins, flavonoids, carbohydrates, alkaloids, and steroids.  1, 1-diphenyl-2-picrylhydrazyl radical scavenging assay proved more sensitive, showing a concentration-dependent increase in antioxidant capacity of the extract, which peaked at 71.8% relative to ascorbic acid (92.9%) at the highest test concentration (400 μg mL-1). The rats tolerated Stereospermum kunthianum extracts with the oral LD50 of more than 5000 mg kg-1. Interestingly, various doses (100-400 mg kg-1) of the extract were effective in causing significant (p≤0.05) reductions in the blood glucose levels of normoglycemic, glucose challenged and streptozotocin-induced diabetic rats when compared to their controls. The results suggest that the stem bark extract of S. kunthianum was a relatively safe herbal extract, rich in phytochemicals, possessed good antioxidant activity, with potential anti-diabetic effects.

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References

Abdu Z, Dimas K, Sunday OA, Sunday D, Elisha BB (2018). In vivo acute toxicity studies and phytochemical screening of stem bark extracts of Detarium microcarpum Guill and per (Caesalpinioideae) and Myosortisscorpioides L. (Boraginaceae). Current Trends in Biomedical Engineering & Biosciences 14(4):555891. https://doi.org/10.19080/CTBEB.2018.14.555891

Albeti KG, Zimmet PZ (1998). Definition, diagnosis and classification of diabetes mellitus and its complications. In: Part 1, diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabetic Medicine 15(7):539-53. http://dx.doi.org/10.1002/(SICI)1096-9136(199807)15:7<539:AID-DIA668>3.0.CO;2-S

Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Analytical Biochemistry 239(1):70-76. https://doi.org/10.1006/abio.1996.0292

Bhattacharjee S, Waqar A, Barua K, Das A, Bhowmik S, Rani S (2018). Phytochemical and pharmacological evaluation of methanolic extract of Lathyrussativus L. seeds. Clinical Phytoscience 4:20. https://doi.org/10.1186/s40816-018-0081-z

Chaudhury A, Duvoor C, Dendi VSR, Kraleti S, Chada A, Ravilla R, … Mirza W (2017) Clinical review of anti-diabetic drugs: Implications for type 2 diabetes mellitus management. Frontiers in Endocrinology (Lausanne) 8:6. https://doi.org/10.3389/fendo.2017.00006

Chin FP, Omogbai EKI, Ozolua RI, Okpo SO (2008). Antidiarrhoeal activities of the aqueous extract of Stereospermum kunthianum (Cham, Sandrine Petit) stem bark in rodents. African Journal of Biotechnology 7(9):1220-1225. https://academicjournals.org›AJB

Ching FP, Omogbai E, Otokiti I (2009). Extract of Stereospermum kunthianum (Cham, Sandrine Petit) protects against generalised seizures in pentylenetetrazole and Electro-convulsive model in Rodents. African Journal of Traditional, Complementary and Alternative Medicine 6(4):544-548. https://doi.org/10.4314/ajtcam.v6i4.57197

Cunha WR, Arantes GM, Ferreira DS, Lucarini R, Silva MLA, Furtado NAJC, … Araujo ARB (2008). Hypoglycemic effect of Leandra lacunose in normal and alloxan- induced diabetic rats. Fitoterapia 79(5):356-60. https://doi.org/10.1016/j.fitote.2008.04.002

Diehl K-H, Hull R, Morton D, Pfister R, Smith YRD, Vidal J-M, …van De Vorstenbosch C (2001). A good practice guide to the administration of substances and removal of blood, including routes and volumes. Journal of Applied Toxicology: An International Journal 21(1):15-23. https://doi.org/10.1002/jat.727

Evans WC (2005). Trease and Evans Pharmacognosy. 15th ed, Saunders Company Ltd, London, pp 223-308.

GBD 2015 Risk Factors Collaborators (2016). Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet (London, England) 388(10053):1659. https://doi.org/10.1016/S0140-6736(16)31679-8

Gill LS (1992). Ethnomedicinal uses of plants in Nigeria. University of Benin Press, Nigeria.

Igoli JO, Ogaji OG, Tor-Anyiin TA, Igoli NP (2005). Traditional medicine practice amongst the Igede people of Nigeria. Part II. African Journal of Traditional, Complementary and Alternative Medicines 2(2):134-152. https://athmsi.org/journalsindex.php/ajtcam/article/view/47/49

Jing L, Ma H, Fan P, Gao R, Jia L (2015). Antioxidant potential, total phenolic and total flavonoid contents of Rhododendron anthoponoides and its protective effect on hypoxia-induced injury in PC12 cells. BMC Complementary and Alternative Medicine 15(1). https://doi.org/10.1186/s12906-015-0820-3

Keay RWJ (1989). Trees of Nigeria. Clarendon Press, Oxford.

Lorke D (1983). A new approach to practical acute toxicity testing. Archives of Toxicology 54:275-28. https://doi.org/10.1007/BF01234480 .

Lin X, Xu Y, Pan X, Xu J, Ding Y, Sun X, … Shan P-F (2020). Global, regional, and national burden and trend of diabetes in 195 countries and territories: an analysis from 1990 to 2025. Scientific Reports 10:14790. https://doi.org/10.1038/s41598-020-71908-9

Mensor LI, Menezes FS, Leitao GG, Reis AO, Dos S, Coube CS, … Leitao SG (2001) Screening of Brazillian plants extract for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research 15:127-130. https://doi.org/10.1002/ptr.687

Mohd IY, Archana S, Arumugam G, Mahmoud A, Kuldeep D (2017) Promising antidiabetic drugs, medicinal plants and herbs: An update. International Journal of Pharmacology 13:732-745. https://doi.org/10.3923/ijp.2017.732.745

National Research Council (2010). Guide for the care and use of laboratory animals. National Academic Press. https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf

OECD (2001). Guideline for testing of chemicals. Acute oral toxicity – Fixed-dose procedure. https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl420.pdf

Okpo SO, Ching FP (2013). Sub-acute toxicity studies on the aqueous extract of Stereospermum kunthianum stem bark (Cham, Sandrine Petit). Journal of Pharmacy & Bioresources 10(2):38-44. https://doi.org/10.4314/jpb.v10i2.1

Papatheodorou K, Banach M, Bekiari E, Rizzo M, Edmonds M (2018). Complications of diabetes 2017. Journal of Diabetes Research 3086167:1-4. https://doi.org/10.1155/2018/3086167

Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Aitavilla D, Bitto A (2017) Oxidative stress: harms and benefits for human health. Oxidative Medicine Cellular Longevity 2017:8416763. https://doi.org/10.1155/2017/8416763

Tropical Plants Database (2014). Retrieved 2021 March 31 from: http://ecocrop.fao.org/ecocrop/srv/en/home Ken Fern. tropical.theferns.info.

Yu JG, Krusznska YT, Mulford MI, Olefsky JM (1999). A comparison of troglitazone and metformin on insulin requirements in euglycemic intensively insulin-treated type 2 diabetic patients. Diabetes 48:2414-2421. https://doi.org/10.2337/diabetes.48.12.2414

World Health Organisation (1985). Diabetes mellitus: Report of a WHO Study Group Geneva. Retrieved 2021 March 20 from https://apps.who.int/iris/handle/10665/39592

Published
2022-09-23
How to Cite
ODE, J. O., NWINYI, F., EGUA, M. O., ONAKPA, M. M., AKANDE, G. M., ONOJA, S. O., MIKAIL, H. G., AKUMKA, D. D., & ADAMU, M. (2022). Hypoglycemic and in vitro antioxidant activities of Stereospermum kunthianum stem bark hydromethanol extract. Notulae Scientia Biologicae, 14(3), 11223. https://doi.org/10.55779/nsb14311223
Section
Research articles
CITATION
DOI: 10.55779/nsb14311223

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