Genetic diversity of Origanum syriacum L. (Lamiaceae) species through Touch-Up Direct Amplification of Minisatellite-region DNA (TU-DAMD) marker
DOI:
https://doi.org/10.55779/nsb14211174Keywords:
genotyping, molecular marker, polymorphism, polymorphic information content (PIC), Syrian oreganoAbstract
Origanum syriacum L. (Lamiaceae) is a perennial species with an important role in medicinal and pharmaceutical applications. However, the attention regarding its genetic diversity is negligible worldwide. Touch-up direct amplification of minisatellite-region DNA (TU-DAMD) marker has been employed to investigate genetic diversity of natural Origanum syriacum L. populations, where 37 wild O. syriacum genotypes were collected from coastal, central and southern regions of Syria. TU-DAMD assay resulted in 188 total bands, of which 152 (80.85%) were polymorphic. The total bands number ranged between 7 and 22 with an average of 11.75 bands/primer. Polymorphic bands number ranged between 4 and 19 with an average of 9.5 polymorphic bands/primer. Moreover, polymorphic information content (PIC) value ranged between 0.051 and 0.322 with an average of 0.225. As for marker index (MI), this value ranged between 0.308 and 5.092 with an average of 2.284. Overall, the present study suggests that the O. syriacum samples collected from coastal region (Lattakia, Jableh, Banyas and Tartous) are genetically distinct from the ones collected from central and southern regions (Homs, Hama, Damascus, Swedah and Qunetra) based on the estimated percent disagreement values (PDV) average of 0.20. Thereby, TU-DAMD test successfully discriminate O. syriacum genotypes showing high genetic diversity within the studied species. In conclusion, TU-DAMD test could be considered as a new tool for studying genetic diversity of other plant species.
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