Genetic diversity of Origanum syriacum L. (Lamiaceae) species through Touch-Up Direct Amplification of Minisatellite-region DNA (TU-DAMD) marker


  • Basel SALEH Atomic Energy Commission of Syria (AECS), Damascus, 6091 (SY)



genotyping, molecular marker, polymorphism, polymorphic information content (PIC), Syrian oregano


Origanum syriacum L. (Lamiaceae) is a perennial species with an important role in medicinal and pharmaceutical applications. However, the attention regarding its genetic diversity is negligible worldwide. Touch-up direct amplification of minisatellite-region DNA (TU-DAMD) marker has been employed to investigate genetic diversity of natural Origanum syriacum L. populations, where 37 wild O. syriacum genotypes were collected from coastal, central and southern regions of Syria. TU-DAMD assay resulted in 188 total bands, of which 152 (80.85%) were polymorphic. The total bands number ranged between 7 and 22 with an average of 11.75 bands/primer. Polymorphic bands number ranged between 4 and 19 with an average of 9.5 polymorphic bands/primer. Moreover, polymorphic information content (PIC) value ranged between 0.051 and 0.322 with an average of 0.225. As for marker index (MI), this value ranged between 0.308 and 5.092 with an average of 2.284. Overall, the present study suggests that the O. syriacum samples collected from coastal region (Lattakia, Jableh, Banyas and Tartous) are genetically distinct from the ones collected from central and southern regions (Homs, Hama, Damascus, Swedah and Qunetra) based on the estimated percent disagreement values (PDV) average of 0.20. Thereby, TU-DAMD test successfully discriminate O. syriacum genotypes showing high genetic diversity within the studied species. In conclusion, TU-DAMD test could be considered as a new tool for studying genetic diversity of other plant species.


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Aboukhalid K, Machon N, Lambourdière J, Abdelkrim J, Bakha M, Douaik A, … Al Faiz C (2017). Analysis of genetic diversity and population structure of the endangered Origanum compactum from Morocco, using SSR markers: implication for conservation. Biological Conservation 212:172-182.

Akeel RW, Rawashdeh IM, Haddad NI, Haddadin M (2009). Genetic variation between and among Origanum syriacum L. and Origanum majorana L. populations collected from different locations in Jordan using RAPD markers. Crop Research (Hisar) 38(1/3):245-257.

Azizi A, Wagner C, Honermeier B, Friedt W (2009) Intraspecific diversity and relationship between subspecies of Origanum vulgare revealed by comparative AFLP and SAMPL marker analysis. Plant Systematics and Evolution 281(1):151-160.

Azizi A, Hadian J, Gholami M, Friedt W, Honermeier B (2012). Correlations between genetic, morphological, and chemical diversities in a germplasm collection of the medicinal plant Origanum vulgare L. Chemistry and Biodiversity 9(12): 2784-801.

Azizi A, Ardalani H, Honermeier B (2016). Statistical analysis of the associations between phenolic monoterpenes and molecular markers, AFLPs and SAMPLs in the spice plant Oregano. Herba Polonica 62(2):42-56.

Botstein D, White RL, Skolnick M, Davis RW (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32(3):314-331.

Chishti S, Kaloo ZA, Sultan P (2013). Medicinal importance of genus Origanum: A review. Journal of Pharmacognosy and Phytotherapy 5(10):170-177.

Degerli S, Tepe B, Celiksoz A, Berk S, Malatyali E (2012). In vitro amoebicidal activity of Origanum syriacum and Origanum laevigatum on Acanthamoeba castellanii cysts and trophozoites. Experimental Parasitology 131:20-24.

De Leonardis W, De Santis C, Fichera G, Fiumara P, Ferrauto G, Noun J, Padulosi S (2007). Carpological and palynological characterization of germplasm of Oregano (Origanum syriacum L.) in Lebanon. Journal of Plant Genetic Resources 20(2):104-113.

Doyle JJ, Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19 (1):11-15.

El-Demerdash ESS, Elsherbeny EA, Salama YAM, Ahmed MZ (2019). Genetic diversity analysis of some Egyptian Origanum and Thymus species using AFLP markers. Journal of Genetic Engineering and Biotechnology 17:13.

El-Gendy AEN, Leonardi M, Mugnaini L, Bertelloni F, Valentina VE, Nardoni S, … Pistelli L (2015). Chemical composition and antimicrobial activity of essential oil of wild and cultivated Origanum syriacum plants grown in Sinai, Egypt. Industrial Crops and Products 67:201- 207.

García-Beltrán JM, Esteban MA (2016). Properties and applications of plants of Origanum Sp. genus. SM Journal of Biology 2(1):1006-1014.

Hadian J, Bigdeloo M, Nazeri V, Khadivi-Khub A (2014). Assessment of genetic and chemical variability in Thymus caramanicus. Molecular Biology Reports 41:3201-3210.

Ibrahim L, Bassal A, El Ezzi A, El Ajouz N, Ismail A, Karaky L, … Ibrahim SK (2012). Characterization and identification of Origanum Spp. from Lebanon using morphological descriptors. World Research Journal of Agricultural Biotechnology 1(1):4-9.

Ietswaart JH (1980). A taxonomic revision of the genus Origanum (Labiatae) 4. Leiden University Press, The Hague, PhD Thesis. pp 153.

Jaccard P (1908). Nouvelles recherches sur la distribution flora. Bulletin de la Societé Vaudoise des Sciences Naturelles 44:223-270.

Karaca M, Ince AG, Aydin A, Elmasulu SY, Turgut K (2015). Microsatellites for genetic and taxonomic research on thyme (Thymus L.). Turkish Journal of Biology 39:147-159.

Karagoz H, Cakmakci R, Hosseinpour A, Ozkan G, Haliloglu K (2020). Analysis of genetic variation and population structure among of oregano (Origanum acutidens L.) accessions revealed by agro-morphological traits, oil constituents and retrotransposon-based inter-primer binding sites (iPBS) markers. Genetic Resources and Crop Evolution 67:1367-1384.

Katsiotis A, Nikoloudakis N, Linos A, Drossou A, Constantinidis T (2009). Phylogenetic relationships in Origanum spp. based on rDNA sequences and intra-genetic variation of Greek O. vulgare subsp. hirtum revealed by RAPD. Scientia Horticulturae 121(1):103-108.

Kintzios SE (2002). Profile of thyme multifaceted prince of hers. In: Kintzios SE (Ed). Oregano: The genera Origanum and Lippia. Medicinal and aromatic plants--industrial profiles. Taylor & Francis, London, pp 3-8.

Loizzo MR, Menichini F, Conforti F, Tundis R, Bonesi M, Saab A, … Frega NG (2009). Chemical analysis, antioxidant, anti-inflammatory and anticholinesterase activities of Origanum ehrenbergii Boiss and Origanum syriacum L. essential oils. Food Chemistry 117:174-180.

Lotti C, Ricciardi L, Rainaldi G, Ruta C, Tarraf W, De Mastro G (2019). Morphological, biochemical, and molecular analysis of Origanum vulgare L. The Open Agriculture Journal 13:116-124.

Lu FA, Ergül A, Arslan M (2006). Assessment of genetic diversity in Turkish oregano (Origanum onites L.) germplasm by AFLP analysis. The Journal of Horticultural Science and Biotechnology 81(1):45-50.

Mouterde PSJ (1983). Nouvelle Flore du Liban et de la Syria. Part 3. Pp 183-188.

Mutu A (2014). Genetic variability within different populations of O. vulgare. Studia Universitatis Moldavi AE 1(71):76-81.

Papaioannou C, Zeliou K, Trigas P, Papasotiropoulos V (2020). High resolution melting (HRM) genotyping in the genus Origanum: Molecular identification and discrimination for authentication purposes. Biochemical Genetics 58:725-737.

Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalsky A (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 2(3):225-238.

Saleh B (2019). Genetic diversity of Salvia tomentosa Miller (Lamiaceae) species using touch-down directed amplification of minisatellite DNA (Td-DAMD) molecular markers. Acta Biologica Szegediensis 63(2):135-141.

Saleh B (2021). TU-DAMD employment for molecular characterization of Salvia judaica and Salvia palaestina species. Acta Biologica Szegediensis 65(1):11-16.

Statsoft (2003). Statistica (Data analysis software system). version 6. Statsoft Inc. 2003.

Tag H, Henedak MM, Abouelhassan EM, Hassan EA, Zayed RA (2020). Antioxidative and hepatoprotective effects of Artemisia santonica and Origanum syriacum against Schistosoma mansoni infection. Journal of Applied Biological Sciences 14(1):86-97.

Taşcıoğlu T, Sadıkoğlu N, Doğanlar S, Frary A (2018). Molecular genetic diversity in the Origanum genus: EST-SSR and SRAP marker analyses of the 22 species in eight sections that naturally occur in Turkey. Industrial Crops and Products 123:746-761.

Tonk FA, Yüce S, Bayram E, Giachino RRA, Sonmez C, Telci I, Furan MA (2010). Chemical and genetic variability of selected Turkish oregano (Origanum onites L.) clones. Plant Systematics and Evolution 288(3):157-165.

Yousefi V, Najaphy A, Zebarjadi A, Safari H (2015). Molecular characterization of Thymus species using ISSR markers. The Journal of Animal and Plant Sciences 25(4):1087-1094.

Zaghloul MS, Poschlod P, Reisch C (2014). Genetic variation in Sinai’s range-restricted plant taxa Hypericum sinaicum and Origanum syriacum subsp. sinaicum and its conservational implications. Plant Ecology and Evolution 147(2):187-201.



How to Cite

SALEH, B. (2022). Genetic diversity of Origanum syriacum L. (Lamiaceae) species through Touch-Up Direct Amplification of Minisatellite-region DNA (TU-DAMD) marker. Notulae Scientia Biologicae, 14(2), 11174.



Research articles
DOI: 10.55779/nsb14211174