Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis

Authors

  • Wei-Seng HO Universiti Malaysia Sarawak, Forest Genomics and Informatics Laboratory (fGiLab), Faculty of Resource Science and Technology, 94300 Kota Samarahan, Sarawak (MY)
  • Wee-Hiang ENG Universiti Malaysia Sarawak, Forest Genomics and Informatics Laboratory (fGiLab), Faculty of Resource Science and Technology, 94300 Kota Samarahan, Sarawak (MY)
  • Kwong-Hung LING Sarawak Timber Association, Wisma STA, 26, Jalan Datuk Abang Abdul Rahim, 93450 Kuching, Sarawak (MY)

DOI:

https://doi.org/10.15835/nsb13310995

Keywords:

ImageJ, mitotic chromosomes, polyploid, Rubiaceae, single and double staining

Abstract

Chromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes obliterated. In the present study, we encountered challenges in obtaining a clear micrograph for the chromosome count of active mitotic cells of Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) root tips. Several obstacles were determined through micrograph observation, such as existing unwanted particles in cells, poor chromosome staining and chromosome clumping. To overcome these, root tip types, staining methodologies, squashing methods were among the factors assessed to obtain clear micrographs. The chromosome counts of N. cadamba under optimized procedure showed 2n = 44 chromosomes. We also apply digital technology in chromosome counts, such as online databases and graphic software that are open source and freely accessible to the public. Only basic laboratory equipment and chemicals were used throughout the study, thus making this study economical and applicable in a basic laboratory. The availability of online digital software and databases provide open-source platforms that will ease the efforts in chromosome count.

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Published

2021-08-18

How to Cite

HO, W.-S., ENG, W.-H., & LING, K.-H. (2021). Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis. Notulae Scientia Biologicae, 13(3), 10995. https://doi.org/10.15835/nsb13310995

Issue

Vol. 13 No. 3 (2021)

Section

Research articles
CITATION
DOI: 10.15835/nsb13310995

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