The effects of methanol and amino acid glycine betaine on qualitative characteristics and yield of sugar beet (Beta vulgaris L.) cultivars

Mehdi  KHOSHKHARAM; Mohamad H. SHAHRAJABIAN; Mehrdad  ESFANDIARY

doi:10.15835/nsb13210949

Authors

Mehdi KHOSHKHARAM Islamic Azad University, Department of Agronomy and Plant Breeding, Isfahan (Khorasgan) Branch, Isfahan (IR)
Mohamad H. SHAHRAJABIAN Chinese Academy of Agricultural Sciences, Biotechnology Research Institute, Beijing 100081 (CN)
Mehrdad ESFANDIARY Islamic Azad University, Department of Agronomy and Plant Breeding, Karaj Branch, Alborz (IR)

DOI:

https://doi.org/10.15835/nsb13210949

Keywords:

catalase, malondialdehyde, nitrate reductase, rubisco, superoxide dismutase

Abstract

In order to investigate the effects of methanol and glycine betaine application on quality traits and yield of different fodder beet cultivars, the experiment was performed as a combined split-factorial design based on randomized complete block design with 3 replications in two years in Isfahan, Iran. The concentrations of methanol in 3 levels (control, 15 and 30% v/v methanol) were considered as the first treatment, the concentrations of glycine betaine in 2 levels (control and 4 g per liter) as the second treatment and different cultivars (‘Sentinel’, ‘Drafter’, ‘Rivolta’, ‘Elanta’, ‘Rasta’, and ‘Qualita’) were considered as the factorial. Foliar spraying was performed at three intervals every two weeks. The results showed that the methanol spraying affected on root yield, sugar, potassium and sodium content, catalase enzyme activity, superoxide dismutase, rubisco, and malondialdehyde significantly. Glycine betaine foliar application showed significant differences in root yield, sugar, potassium and sodium content, enzyme catalase activity, superoxide dismutase, rubisco, and malondialdehyde. Based on the results, the utilization of methanol and glycine betaine caused quality improvement of the sugar beet under similar conditions of the present experiment.

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References

Achkor H, Díaz M, Fernández MR, Biosca JA, Parés X, Martínez MC (2003). Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis. Plant Physiology 132:2248-2255. https://doi.org/10.1104/pp.103.022277

Aebi H (1984). Catalase in vitro. Methods in Enzymology 105:121-126. https://doi.org/10.1016/s0076-6879(84)05016-3

Albayrak S, Camas N (2007). Effects of temperature and light intensity on growth of fodder beet (Beta vulgaris L. var. crassa Mansf.). Bangladesh Journal of Botany 36:1-12. https://doi.org/10.3329/bjb.v36i1.1542

Amin GA, Badr EA, Afifi M (2013). Root yield and quality of sugar beet (Beta vulgaris L.) in response to biofertilizer and foliar application with micronutrients. World Applied Sciences Journal 27:1385-1389.

Ashraf M, Foolad MR (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59:206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006

Ashraf M, Nawaz K, Raza S (2008). Growth enhancement in two potential cereal crops, maize and wheat, by exogenous applicationation of glycinebetaine. In: Biosaline Agriculture and High Salinity Tolerance. Springer, pp 21-35. https://doi.org/10.1007/978-3-7643-8554-5_2

Behrouzyar EK, Yarnia M (2016). Effects of methanol foliar application on nutrient content and RWC of sugar beet under water deficit stress. Bangladesh Journal of Botany 45:1069-1074.

Blokhina O, Virolainen E, Fagerstedt KV (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany 91:179-194. https://doi.org/10.1093/aob/mcf118

Bose A, Ghosh B (1995). Effect of heat stress on ribulose 1, 5-bisphosphate carboxylase in rice. Phytochemistry 38:1115-1118.

Cha-um S, Rai V, Takabe T (2019). Proline, glycinebetaine, and trehalose uptake and inter-organ transport in plants under stress. In: Osmoprotectant-Mediated Abiotic Stress Tolerance in Plants. Springer, pp 201-223. https://doi.org/10.1007/978-3-030-27423-8_9

Chen Q, Zhang M, Shen S (2011). Effect of salt on malondialdehyde and antioxidant enzymes in seedling roots of Jerusalem artichoke (Helianthus tuberosus L.). Acta Physiologiae Plantarum 33:273-278. https://doi.org/10.1007/s11738-010-0543-5

Chen THH, Murata N (2008). Glycinebetaine: an effective protectant against abiotic stress in plants. Trends in Plant Science 13:499-505. https://doi.org/10.1016/j.tplants.2008.06.007

Crafts-Brandner S, Law R (2000). Effect of heat stress on the inhibition and recovery of the ribulose-1, 5-bisphosphate carboxylase/oxygenase activation state. Planta 212:67-74.

Cruz F, Castro GLS, Júnior DS, Festucci-Buselli RA, Pinheiro HA (2013). Exogenous glycine betaine modulates ascorbate peroxidase and catalase activities and prevent lipid peroxidation in mild water-stressed Carapa guianensis plants. Photosynthetica 51:102-108.

Cuin TA, Shabala S (2007). Compatible solutes reduce ROS‐induced potassium efflux in Arabidopsis roots. Plant, Cell & Environment 30:875-885.

Darwesh OM, Sultan YY, Seif MM, Marrez DA (2018). Bio-evaluation of crustacean and fungal nano-chitosan for applying as food ingredient. Toxicology Reports 5:348-356. https://doi.org/10.1016/j.toxrep.2018.03.002

Demmers-Derks H, Mitchell RAC, Mitchell VJ, Driscoll SP, Gibbard C, Lawlor DW (1996). Sugar beet under climatic change: photosynthesis and production. Aspects of Applied Biology 45:163-170.

Dorokhov YL, Sheshukova EV, Komarova TV (2018). Methanol in plant life. Frontiers in Plant Science 9:1-16. https://doi.org/10.3389/fpls.2018.01623

Fall R, Benson AA (1996). Leaf methanol-the simplest natural product from plants. Trends in Plant Science 1:296-301. https://doi.org/10.1016/s1360-1385(96)88175-0

Felix JD, Roebuck JA, Mead RN, Willey JD, Avery Jr GB, Kieber RJ (2019). Methanol and ethanol concentrations in a Greenland ice core. Atmospheric Environment 217:116948. https://doi.org/10.1016/j.atmosenv.2019.116948

Fu J, Huang B (2001). Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany 45:105-114.

Garrow TA (2015). Betaine-homocysteine S-methyltransferase. In: Betaine: Chemistry, Analysis, Function and Effects. The Royal Society of Chemistry, Chapter 7, pp 90-101. http://dx.doi.org/10.1039/9781782628446-00090

Grotto D, Maria LS, Valentini J, Paniz C, Schmitt G, Garcia SC, ... Farina M (2009). Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quimica Nova 32:169-174. https://doi.org/10.1590/s0100-40422009000100032

Hadir S, Gaiser T, Hüging H, Athmann M, Pfarr D, Kemper R, ... Seidel S (2021). Sugar beet shoot and root phenotypic plasticity to nitrogen, phosphorus, potassium and lime omission. Agriculture 11:1-1. https://doi.org/10.3390/agriculture11010021

Haslam R, Rust S, Pallett K, Cole D, Coleman J (2002). Cloning and characterisation of S-formylglutathione hydrolase from Arabidopsis thaliana: a pathway for formaldehyde detoxification. Plant Physiology Biochemistry 40:281-288. https://doi.org/10.1016/s0981-9428(02)01378-5

Hasunuma T, Fukusaki E-i, Kobayashi A (2004). Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration in cell wall metabolism and a dwarf phenotype. Journal of Biotechnology 111:241-251. https://doi.org/10.1016/j.jbiotec.2004.04.015

Hoffmann C (2014). Adaptive responses of Beta vulgaris L. and Cichorium intybus L. root and leaf forms to drought stress. Journal of Agronomy and Crop Science 200:108-118.

Hoffmann CM, Leijdekkers M, Ekelöf J, Vancutsem F (2018). Patterns for improved storability of sugar beet–importance of marc content and damage susceptibility of varieties in different environments. European Journal of Agronomy 101:30-37.

Hsiao TC (2000). Leaf and root growth in relation to water status. HortScience 35:1051-1057.

Iqbal N, Ashraf M, Ashraf M (2008). Glycine betaine, an osmolyte of interest to improve water stress tolerance in sunflower (Helianthus annuus L.): water relations and yield. South African Journal of Botany 74:274-281. https://doi.org/10.1016/j.sajb.2007.11.016

Joshi R, Wani SH, Singh B, Bohra A, Dar ZA, Lone AA, ... Singla-Pareek SL (2016). Transcription factors and plants response to drought stress: current understanding and future directions. Frontiers in Plant Science 7:1029. https://doi.org/10.3389/fpls.2016.01029

Kadkhodaei H, Sodaeizadeh H, Arany AM, Hakim Zadeh MA (2016). The role of glycine betaine in increasing drought resistance of Sorghum halopens under field condition. Environmental Stresses in Crop Sciences 9:139-147.

Keles Y, Öncel I (2004). Growth and solute composition in two wheat species experiencing combined influence of stress conditions. Russian Journal of Plant Physiology 51:203-209.

Khan SU, Khan A, Naveed S (2015). Effect of exogenously applied kinetin and glycine betaine on metabolic and yield attributes of rice (Oryza sativa L.) under drought stress. Emirates Journal of Food and Agriculture 75-81.

Kordic S, Cummins I, Edwards R (2002). Cloning and characterization of an S-formylglutathione hydrolase from Arabidopsis thaliana. Archives of Biochemistry 399:232-238. https://doi.org/10.1006/abbi.2002.2772

Koukourikou-Petridou M, Koukounaras A (2002). The effect of methanol on the growth and chlorophyll content of tomato and pepper. International Society for Horticultural Science (ISHS), Leuven, Belgium. pp 271-274. https://doi.org/10.17660/ActaHortic.2002.579.45

Kumar RR, Dubey K, Goswami S, Hasija S, Pandey R, Singh PK, ... Praveen S (2020). Heterologous expression and characterization of novel manganese superoxide dismutase (Mn-SOD) – A potential biochemical marker for heat stress-tolerance in wheat (Triticum aestivum). International Journal of Biological Macromolecules 161:1029-1039. https://doi.org/10.1016/j.ijbiomac.2020.06.026

Kurepin LV, Ivanov AG, Zaman M, Pharis RP, Allakhverdiev SI, Hurry V, Hüner NP (2015). Stress-related hormones and glycinebetaine interplay in protection of photosynthesis under abiotic stress conditions. Photosynthesis Research 126:221-235. https://doi.org/10.1007/s11120-015-0125-x

Ma XL, Wang YJ, Xie SL, Wang C, Wang W (2007). Glycinebetaine application ameliorates negative effects of drought stress in tobacco. Russian Journal of Plant Physiology 54:472-479. https://doi.org/10.1134/s1021443707040061

Mäck G, Hoffmann CM (2006). Organ-specific adaptation to low precipitation in solute concentration of sugar beet (Beta vulgaris L.). European Journal of Agronomy 25:270-279. https://doi.org/10.1016/j.eja.2006.06.004

Mäkelä P (2004). Agro-industrial uses of glycinebetaine. Sugar Tech 6:207-212. https://doi.org/10.1007/bf02942500

Mäkelä P, Kärkkäinen J, Somersalo S (2000). Effect of glycinebetaine on chloroplast ultrastructure, chlorophyll and protein content, and RuBPCO activities in tomato grown under drought or salinity. Biologia Plantarum 43:471-475. https://doi.org/10.1023/a:1026712426180

Mäkelä P, Peltonen-Sainio P, Jokinen K et al. (1996). Uptake and translocation of foliar-applied glycinebetaine in crop plants. Plant Science 121:221-230. https://doi.org/10.1016/s0168-9452(96)04527-x

McGiffen ME, Manthey JA (1996). The role of methanol in promoting plant growth: A current evaluation. HortScience 31:1092-1096.

Nadali I, Paknejad F, Moradi F, Vazan S, Tookalo M, Jami Al-Ahmadi M, Pazoki A (2010). Effects of methanol on sugar beet (Beta vulgaris). Australian Journal of Crop Science 4:398-401.

Nawaz M, Wang Z (2020). Abscisic acid and glycine betaine mediated tolerance mechanisms under drought stress and recovery in Axonopus compressus: A new insight. Scientific Reports 10:6942. https://doi.org/10.1038/s41598-020-63447-0

Nonomura A, Benson A (1992). The path of carbon in photosynthesis: improved crop yields with methanol. Proceedings of the National Academy of Sciences 89:9794-9798. https://doi.org/10.1073/pnas.89.20.9794

Ohnishi N, Murata N (2006). Glycinebetaine counteracts the inhibitory effects of salt stress on the degradation and synthesis of D1 protein during photoinhibition in Synechococcus sp. PCC 7942. Plant Physiology 141:758-765. https://doi.org/10.1104/pp.106.076976

Park E-J, Jeknic Z, Chen TH (2006). Exogenous application of glycinebetaine increases chilling tolerance in tomato plants. Plant and cell physiology 47:706-714.

Pi Z, Stevanato P, Sun F, Yang Y, Sun X, Zhao H, ... Yu L (2016). Proteomic changes induced by potassium deficiency and potassium substitution by sodium in sugar beet. Journal of Plant Research 129:527-538. https://doi.org/10.1007/s10265-016-0800-9

Prajapat P, Singh D, Tripathi S, Patel K, Abbas H, Patel A (2018). Effect of water stress on antioxidative enzymes and glycine betaine content in drought tolerant and drought susceptible cotton (Gossypium hirsutum L.) genotypes. Indian Journal of Biochemistry and Biophysics (IJBB) 3:198-204.

Rehman A, Khalil SK (2018). Effect of exogenous application of salicylic acid, potassium nitrate and methanol on canola growth and phenology under different moisture regimes. Sarhad Journal of Agriculture 34:781-789. https://doi.org/10.17582/journal.sja/2018/34.4.781.789

Reinhold L, Kaplan A (1984). Membrane transport of sugars and amino acids. Annual Review of Plant Physiology 35:45-83. https://doi.org/10.1146/annurev.pp.35.060184.000401

Romandini P, Bonotto C, Bertoloni G, Beltramini M, Salvato B (1994). Superoxide dismutase, catalase and cell dimorphism in Candida albicans cells exposed to methanol and different temperatures. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology 108:53-57. https://doi.org/10.1016/1367-8280(94)90089-2

Saneienejad A, Tohidi M, Khaniani BH, Sadeghi M (2019). Effect of Foliar application of methanol on changes of antioxidant enzymes of Vigna unguiculata L. in water-deficit stress. Agricultural Science Digest 39:296-300. https://doi.org/10.18805/ag.d-169

Sheshukova EV, Komarova TV, Pozdyshev DV, Ershova NM, Shindyapina AV, Tashlitsky VN, ... Dorokhov YL (2017). The intergenic interplay between aldose 1-epimerase-like protein and pectin methylesterase in abiotic and biotic stress control. Frontiers in Plant Science 8:1-17. https://doi.org/10.3389/fpls.2017.01646

Stepanov SS, Zolotareva EK, Belyavskaya NA (2020). The role of catalase in assimilation of exogenous methanol by Chlamydomonas reinhardtii cells. Journal of Applied Phycology 1-10.

Stewart RR, Bewley JD (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology 65:245-248. https://doi.org/10.1104/pp.65.2.245

Branch K (2009). Effect of super absorbent application on antioxidant enzyme activities in canola (Brassica napus L.) cultivars under water stress conditions. American Journal of Agricultural and Biological Sciences 4:215-223. https://doi.org10.3844/ajabssp.2009.215.223

Valizadeh-Kamran R, Vojodi Mehrabani L, Pessarakli M (2019). Effects of foliar application of methanol on some physiological characteristics of Lavandula stoechas L. under NaCl salinity conditions. Journal of Plant Nutrition 42:261-268. https://doi.org/10.1080/01904167.2018.1554677

Wiśniewska A, Andryka-Dudek P, Czerwiński M, Choulj D (2019). Fodder beet is a reservoir of drought tolerance alleles for sugar beet breeding. Plant Physiology and Biochemistry 145:120-131. https://doi.org/10.1016/j.plaphy.2019.10.031

Yang C, Zhou Y, Fan J, Fu Y, Shen L, Yao Y, ... Guo J (2015). SpBADH of the halophyte Sesuvium portulacastrum strongly confers drought tolerance through ROS scavenging in transgenic Arabidopsis. Plant Physiology Biochemistry 96:377-387. https://doi.org/10.1016/j.plaphy.2015.08.010

Zbieć I, Karczmarczyk S, Podsiadło C (2003). Response of some cultivated plants to methanol as compared to supplemental irrigation. Electronic Journal of Polish Agricultural Universities 6:1-7.